Quantitation of Chill-Induced Release of a Tubulin-Like Factor and Its Prevention by Abscisic Acid in <i>Gossypium hirsutum</i> L.

Rikin, Arnon; Atsmon, Dan; Gitler, Carlos

doi:10.1104/pp.71.4.747

Cited by 46 publications

(21 citation statements)

References 20 publications

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“…Although induction of chilling tolerance with ABA in maize plants has not been demonstrated (29), ABA has been reported to increase chilling tolerance of eggplant, soybean, and cucumber seedlings (5,15,21), cotton cotyledonary discs (19,20), and maize callus culture (4). The inability of ABA to induce chilling tolerance in maize plants could result from lack of uptake or biodegradation of applied exogenous ABA.…”

Section: Discussionmentioning

confidence: 99%

“…However, cold acclimation results in injury, including death of chillingsensitive plants such as maize. ABA induces chilling tolerance, and the induction occurs only in a warm temperature regimen (5,20,21). It is not known whether the enhancement of chilling tolerance by ABA in maize cultured cells is also associated with the expression of ABA-induced genes.…”

mentioning

confidence: 99%

“…Zhang et al (29) proposed that increases in endogenous ABA before chilling may be an essential step in inducing chilling tolerance. Although direct application of ABA to maize plants was unsuccessful in reducing chilling injury (29), ABA has been shown to reduce chilling injury in a number ofother chilling-sensitive plants (5,10,(19)(20)(21) and in maize callus culture (4). (17).…”

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confidence: 99%

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Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

Xin¹,

Li²

1992

Plant Physiol.

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The induction of chilling tolerance by abscisic acid (ABA) in maize (Zea mays L. cv Black Mexican Sweet) suspension cultured cells was examined. Cell viability during exposure to chilling was estimated by triphenyl tetrazolium chloride reduction immediately after chilling and a filter paper growth assay. Both methods yielded comparable results. Chilling tolerance was induced by transferring 5-day-old cultures (late log phase) to a fresh medium containing ABA (10 to 100 micromolar). The greatest chilling tolerance was achieved with ABA at 100 micromolar. Growth of cells was inhibited at this concentration. After a 7-day exposure to 40C in the dark, the survival of ABA-treated cells (100 micromolar ABA, 280C for 24 h in the dark) was sevenfold greater than untreated cells. Effective induction of chilling tolerance was first observed when cells were held at 280C for 6 hours after adding ABA. No tolerance was induced if the culture was chilled at the inception of ABA treatment. Induction of chilling tolerance was inhibited by cycloheximide. These results indicate that ABA is capable of inducing chilling tolerance when ABA-treated cells are incubated at a warm temperature before exposure to chilling, and this induction requires de novo synthesis of proteins.

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Section: Discussionmentioning

confidence: 99%

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Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

Xin¹,

Li²

1992

Plant Physiol.

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“…Several chemical compounds, ions, and environmental conditions shift the equilibrium toward depolymerization (8 Freezing promotes microtubule depolymerization in several plants including onion (4), rye (10), spinach (3), and winter rape (Boyang Chu, personal communication). Low temperature also promotes microtubule depolymerization in a variety ofplant species (3,4,10,14). The implications of microtubule depolymerization induced by low temperature and freezing, however, are not well known.…”

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confidence: 99%

Effect of Microtubule Stabilization on the Freezing Tolerance of Mesophyll Cells of Spinach

Bartolo¹,

Carter²

1991

Plant Physiol.

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Freezing, dehydration, and supercooling cause microtubules in mesophyll cells of spinach (Spinacia oleracea L. cv Bloomsdale) to depolymerize (ME Bartolo, JV Carter, Plant Physiol [1991] 97: 175-181). The objective of this study was to determine whether the LT50 (lethal temperature: the freezing temperature at which 50% of the tissue is killed) of spinach leaf tissue can be changed by diminishing the extent of microtubule depolymerization in response to freezing. Also examined was how tolerance to the components of extracellular freezing, low temperature and dehydration, is affected by microtubule stabilization. Leaf sections of nonacclimated and cold-acclimated spinach were treated with 20 micromolar taxol, a microtubule-stabilizing compound, prior to freezing, supercooling, or dehydration. Taxol stabilized microtubules against depolymerization in cells subjected to these stresses. When pretreated with taxol both nonacclimated and cold-acclimated cells exhibited increased injury during freezing and dehydration. In contrast, supercooling did not injure cells with taxol-stabilized microtubules. Electrolyte leakage, visual appearance of the cells, or a microtubule repolymerization assay were used to assess injury. As leaves were cold-acclimated beyond the normal period of 2 weeks taxol had less of an effect on cell survival during freezing. In leaves acclimated for up to 2 weeks, stabilizing microtubules with taxol resulted in death at a higher freezing temperature. At certain stages of cold acclimation, it appears that if microtubule depolymerization does not occur during a freeze-thaw cycle the plant cell will be killed at a higher temperature than if microtubule depolymerization proceeds normally. An alternative explanation of these results is that taxol may generate abnormal microtubules, and connections between microtubules and the plasma membrane, such that normal cellular responses to freeze-induced dehydration and subsequent rehydration are blocked, with resultant enhanced freezing injury.Microtubules are dynamic structures, existing in an equilibrium between soluble tubulin subunits and the polymerized filament. Several chemical compounds, ions, and environmental conditions shift the equilibrium toward depolymerization (8). Included in the list of destabilizing agents are freezing and low temperature.

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“…, and (d) stabilizing microtubule cytoskeleton networks to strengthen cell organization (Rikin et al, 1979(Rikin et al, , 1983. Xin and Li (1992) characterized the induction of chilling tolerance by ABA in maize suspension-cultured cells.…”

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confidence: 99%

Alteration of Gene Expression Associated with Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

Xin

1993

Plant Physiol.

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Quantitation of Chill-Induced Release of a Tubulin-Like Factor and Its Prevention by Abscisic Acid in Gossypium hirsutum L.

Cited by 46 publications

References 20 publications

Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

Effect of Microtubule Stabilization on the Freezing Tolerance of Mesophyll Cells of Spinach

Alteration of Gene Expression Associated with Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

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