Alteration of Gene Expression Associated with Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells

Relationship between Proline and Abscisic Acid in the Induction of Chilling Tolerance in Maize Suspension-Cultured Cells

“…5, A and C). We have reported that ABA increased the net synthesis of 11 proteins at 28°C (Xin and Li, 1993). None of these ABA-regulated proteins showed any detectable changes in Pro-treated cells (Fig.…”

“…Figure 6 shows that no specific alterations in protein synthesis were found in Pro-treated cells such as were observed in the ABA-treated cells during the chilling tolerance induction (Xin and Li, 1993).…”

“…We reported that the abundance of several proteins is increased by the ABA treatment, and this increase is associated with the increase in chilling tolerance (Xin and Li, 1993). Here, we compared the protein synthesis in cells treated with 100 m~ Pro and the protein synthesis in ABA-treated cells.…”

“…Protein synthesis after Pro treatment was analyzed by twodimensional PAGE according to Xin and Li (1993). At 10 h after Pro (100 mM) treatment, 2 mL of culture was transferred to a sterile 5-mL plastic culture tube containing 100 juCi of L-[ 35 S]Met (>1000 Ci mmol ~').…”

“…At 10 h after Pro (100 mM) treatment, 2 mL of culture was transferred to a sterile 5-mL plastic culture tube containing 100 juCi of L-[ 35 S]Met (>1000 Ci mmol ~'). After 2 h of incubation at 28°C, cells were collected for protein extraction and analysis on two-dimensional PAGE (Xin and Li, 1993).…”