Quantitation of Aryltetralin Lignans in Plant Parts and among Different Populations of <i>Podophyllum </i><i>peltatum</i> by Reversed-Phase High-Performance Liquid Chromatography

Bastos, Jairo K.; Burandt, Charles L.; Nanayakkara, N. P. Dhammika; Bryant, Laura; McChesney, James D.

doi:10.1021/np960155d

Cited by 35 publications

(23 citation statements)

References 9 publications

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“…CE quantification experiments of different Podophyllure peltatum samples showed similar results compared to those obtained by HPLC [7] ( Table I). The peaks were assigned by their UV-spectra, was well as by spiking the samples with the pure compounds.…”

Section: Figuresupporting

confidence: 73%

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Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

1999

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SummarySeparation of the main Podophyllum lignans by MECC has been achieved using a phosphate-borate buffer system with the pH of 7.25, containing 25 mM SDS and 15 % methanol. Optimum temperature and voltage were found to be 35 ~ and 20 kV, respectively, UVdetection was performed at 220 nm. The method was successfully used to analyse different samples of Podophyllum peltatum collected in the southern USA, and showed great qualitative and quantitative variations among these populations. The quantitative MECC results for podophyllotoxin were in good agreement to those obtained by HPLC.

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Section: Figuresupporting

confidence: 73%

“…Analytical methods described in literature are based on TLC and HPLC [5][6][7]. In one report [8] the root extract of P. emodi has been analyzed by means of CE.…”

Section: Introductionmentioning

confidence: 99%

Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

1999

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“…The rhizome contains podophyllotoxin (PTOX) possessing anti-cancer properties (Jackson et al 1984a;Bastos et al 1996;Moraes et al 2002). The semi-synthetic derivatives of this compound like etoposide, etophos, and teniposide are used clinically as chemotherapeutic agents for a variety of tumors, including small cell lung carcinoma, testicular cancer, and malignant lymphoma (Stähelin and von Wartburg 1991;Damayanthi and Lown 1998).…”

Section: Introductionmentioning

confidence: 99%

Podophyllotoxin production via cell and adventitious root cultures of Podophyllum peltatum

Anbazhagan

Ahn

Harada

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Podophyllum peltatum is an important medicinal plant that produces podophyllotoxin (PTOX) with anticancer properties. We established the embryogenic cell and adventitious root culture systems in P. peltatum and analyzed PTOX production. For the growth of embryogenic cell clumps in shake flask culture, the most efficient concentration of 2,4-dichloroacetic acid (2,4-D) was 6.78 μM, and the growth of embryogenic cell clumps was 15.9-fold increased in Murashige and Skoog MS liquid medium with 6.78 μM 2,4-D after 3 wk of culture. To induce adventitious roots, half-strength MS medium showed the best results for adventitious root induction compared to full strength MS medium and MS medium lacking NH 4 NO 3 . Optimal indole-3-butyric acid concentration for adventitious root formation was 14.78 μM. In liquid medium, the frequency of adventitious root formation from root segments was 87.7% and the number of laterally formed adventitious roots was 22.3 per segment. PTOX production in embryogenic cells and adventitious roots was confirmed by liquid chromatography and electrospray ionization-tandem mass spectrometry analysis. Highperformance liquid chromatography analysis revealed that adventitious roots contained higher PTOX than embryogenic cell clumps. Elicitor treatment (20 μM methyl jasmonate) strongly enhanced the production of PTOX in both embryogenic cell clumps and adventitious roots. This observation suggests that both embryogenic cell and adventitious root culture can be adopted to produce PTOX.

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“…Podophyllotoxin production is not restricted to the roots as low amounts of podophyllotoxin (0.003-0.229% d.w.) are also found in P. hexandrum leaves (Pandey et al 2013). Furthermore, several researchers reported podophyllotoxin production in the leaves of P. peltatum (Bastos et al 1996;Moraes et al 2000Moraes et al , 2002Cushman et al 2006;Zheljazkov et al 2011). The most recent population study was by Zheljazkov and coworkers who found up to 2.53% (d.w.) podophyllotoxin (Zheljazkov et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

et al. 2017

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Background and aim The endangered Podophyllum hexandrum is an important industrial source of podophyllotoxin, which is a precursor for the anticancer drugs etoposide and teniposide. Attempts to obtain podophyllotoxin through cell cultures or chemical synthesis have still a long way to go before being economical feasible. The objective of this study was to increase the root formation and podophyllotoxin production of P. hexandrum cultivated in a glasshouse. Methods Root formation and podophyllotoxin production of P. hexandrum in sand or peat-perlite soil at 15°C or 25°C was determined. Furthermore, the influence of methyl jasmonate on the podophyllotoxin production was determined. Results More root formation was observed in peat-perlite soil than in sand soil. Furthermore, root formation was higher at 15°C than at 25°C. This resulted in the highest podophyllotoxin production per plant in peat-perlite at 15°C (160 ± 22 mg/plant d.w.). Furthermore, methyl jasmonate treatment of the leaves increased the podophyllotoxin production in the roots by 21%. Conclusion We were able to cultivate P. hexandrum in a glasshouse in the Netherlands and improve the root formation and podophyllotoxin production. This paves the way for large-scale cultivation of P. hexandrum in the temperate latitudes for the production of the pharmaceutical interesting podophyllotoxin.

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Quantitation of Aryltetralin Lignans in Plant Parts and among Different Populations of Podophyllum peltatum by Reversed-Phase High-Performance Liquid Chromatography

Cited by 35 publications

References 9 publications

Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

Podophyllotoxin production via cell and adventitious root cultures of Podophyllum peltatum

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

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