Quantitation by1H-NMR of dolichol, cholesterol and choline-containing lipids in extracts of normal and phathological thyroid tissue

Yoshioka, Yoshichika; Sasaki, Jun; Yamamoto, Masahide; Saitoh, Kazuyoshi; Nakaya, Shigeyuki; Kubokawa, Masaru

doi:10.1002/1099-1492(200011)13:7<377::aid-nbm658>3.0.co;2-e

Cited by 47 publications

(37 citation statements)

References 34 publications

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“…As PAF is believed to be involved in tissue injury and cancer, it was of interest to investigate its putative involvement in both normal and diseased thyroid tissue from patients with a hyperplastic goitre, to patients with a benign adenoma and finally patients with a thyroid cancer. Previous authors reported alterations in phospholipids levels in blood and tumour tissue of patients with thyroid carcinoma (5,6). Furthermore, as we recently observed, alterations are found in the PAF production/degradation pathways in the tumour tissue of patients with lung and colorectal carcinoma (21,23,24).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, several lipid molecules (such as phosphatidic acid, cyclic lysophosphatidic acid, ceramide and sphingosine 1-phosphate) that act as both intracellular messengers and extracellular mediators are reported to affect tumour development and neoplasia (2 -4). Alterations in phospholipid concentrations are reported in plasma and thyroid tissue of patients with thyroid carcinoma (5,6), and lysophosphatidic acid is a potent growth factor for human thyroid cells (7). The lipidic molecule platelet-activating factor (PAF) sparks a wide range of immunoregulatory actions (8).…”

Section: Introductionmentioning

confidence: 99%

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Platelet-activating factor and human thyroid cancer

Denizot¹,

Chianea²,

Labrousse³

et al. 2005

eur j endocrinol

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Objective: Platelet-activating factor (PAF) is a pro-inflammatory and angiogenic lipid mediator involved in several types of cancer in humans. The levels of PAF, lyso-PAF (the PAF precursor), phospholipase A 2 activity (PLA 2 , the enzymatic activity implicated in lyso-PAF formation) and acetylhydrolase activity (AHA, the PAF-degrading enzyme) were investigated in various diseased thyroid tissues. Subjects: Control and diseased tissue of patients with a hyperplastic goitre (n ¼ 14), a benign adenoma (n ¼ 12) and a papillary thyroid carcinoma (n ¼ 15) were investigated. Results: PAF receptor transcripts were found in the human thyroid tissue. PAF, lyso-PAF, PLA 2 and AHA were present in control thyroid tissues, their levels being significantly correlated with each other, suggesting tiny regulations of the PAF metabolic pathways inside the thyroid gland. PAF, lyso-PAF, PLA 2 and AHA levels remained unchanged in diseased tissues of patients with a hyperplastic goitre, a benign adenoma and a papillary thyroid carcinoma. No difference was found between PAF, lyso-PAF, PLA 2 and AHA levels with respect to the TNM tumour status and the histological subtype of papillary thyroid carcinoma. No correlation was found between tissue PAF levels and those of vascular endothelial growth factor and basic fibroblast growth factor, two angiogenic growth factors involved in thyroid cancer and that mediate their effect through PAF release in breast and colorectal cancer. Conclusion: PAF, PAF receptor transcripts and the enzymatic activities implicated in PAF production and degradation are present in the thyroid gland. While the physiological role of PAF is presently unknown in thyroid physiology, this study highlights no evidence for a potentially important role of PAF during human thyroid cancer, a result that markedly differs from breast and colorectal ones.European Journal of Endocrinology 153 31-40

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Platelet-activating factor and human thyroid cancer

Denizot¹,

Chianea²,

Labrousse³

et al. 2005

eur j endocrinol

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“…3). These resonances have previously been used for quantification of cholesterol and cholesteryl esters (Pollesello et al, 1992;Tosi et al, 2003;Yoshioka et al, 2000) in human biological materials. In the present study, the ratios between cholesterol and cholesteryl esters obtained from the spectra of milt were 1.0 before storage and 0.3 after storage.…”

Section: Changes In Cholesterol During Storagementioning

confidence: 99%

“…The 13 C chemical shifts of polar head groups of different phospholipids, have previously been assigned (Everts and Davies, 2000;Jimeno et al, 2002;Falch et al, 2006). 1 H NMR has been shown to provide quantitative data of n-3 fatty acids and DHA (Igarashi et al, 2000), cholesterol (Pollesello et al, 1992;Tosi et al, 2003;Yoshioka et al, 2000), and semi-quantitative data of reaction products from lipid oxidation (Falch et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

High resolution NMR for studying lipid hydrolysis and esterification in cod (Gadus morhua) gonads

Falch

Størseth

Aursand

2007

Chemistry and Physics of Lipids

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“…Several other authors have used slight modification of these two methods in their studies (Bates et al 1989;Fan et al 1986;J.E. et al 2002;Yoshichika et al 2000;Johnson and Shoolery 1962;Sijens et al 1996;Moyec et al 2000).…”

Section: Discussionmentioning

confidence: 99%

An Improved, Single Step Standardized Method of Lipid Extraction from Human Skeletal Muscle Tissue

et al. 2006

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A new method of lipid extraction from human skeletal muscle is presented. The quantitative extraction of lipid metabolites such as phospholipids, triglycerides and cholesterol, simultaneously, in a single experiment is evaluated by 1 H-NMR spectroscopy. Thirty-seven normal cryopreserved muscle samples were pulverized at 297 liquid nitrogen temperature and treated with methanol and chloroform at different ratios (i.e., 1 : 2, 1 : 3, and 1 : 4, by vol). The methanol-chloroform ratio, at which maximum extraction occurred (1 : 3, by vol), was further used to study the efficiency of lipid extraction by simultaneous and sequential treatment of the solvents; phospholipids and cholesterol were extracted better when the tissue was treated first with methanol and subsequently, with chloroform. Further, the quantities of the lipid extracts obtained from muscle tissue by treatment of methanol and chloroform sequentially (1 : 3 ratio, by vol) with and without addition of a known quantity of standard lipids, were compared with those obtained from commonly used methods (Folch's and Bligh and Dyer's). The present method of sequential use of methanol and chloroform in 1 : 3 ratio provided 44.9%, 41.9%, and 22.8% higher yields of triglyceride, phospholipids, and cholesterol, respectively, compared to Folch's method and 73.5%, 54.1%, and 46.9% higher yields compared to Bligh and Dyer's method in spite of adding yield of re-extraction in the latter two methods. In the recovery experiment after addition of 1 and 2 mg of these individual lipid components, average recovery was 84.5% with the present method compared to 70.7% with Folch's method and 62.2% with the Bligh and Dyer's method. The results show that the present method is superior in terms of extraction of lipid components such as phospholipids, triglycerides, and cholesterol compared to commonly used methods. The major advantage of this method is that the total lipids are extracted in a single step.

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Quantitation by1H-NMR of dolichol, cholesterol and choline-containing lipids in extracts of normal and phathological thyroid tissue

Cited by 47 publications

References 34 publications

Platelet-activating factor and human thyroid cancer

Platelet-activating factor and human thyroid cancer

High resolution NMR for studying lipid hydrolysis and esterification in cod (Gadus morhua) gonads

An Improved, Single Step Standardized Method of Lipid Extraction from Human Skeletal Muscle Tissue

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