QUANTIFYING VESICULAR‐ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION <sup>*</sup>

Biermann, Brenda J.; Linderman, R. G.

doi:10.1111/j.1469-8137.1981.tb01690.x

Cited by 380 publications

(152 citation statements)

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“…The cooled root samples were washed and cut into 0.5 to 1.0-cm-long segments and stained with 0.5% (w/v) acid fuchsin (Biermann and Linderman 1981). Thirty root fragments (approximately 1 cm long) were mounted on slides in a polyvinyl alcohol-lactic acid-glycerol solution (Koske and Tessier 1983) and examined at 100-400× magnification under an Olympus BX50 microscope with an automatic photomicrographic system for the presence of AM fungal structures.…”

Section: Assessment Of Am Colonizationmentioning

confidence: 99%

Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan island, China

et al. 2005

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Species richness, spore density, frequency of occurrence, and relative abundance of AM fungi were determined in rhizosphere soil samples from nine tropical rainforest sites on Hainan island, south China, and the arbuscular mycorrhizal (AM) status of members of the Meliaceae was examined. All 28 plant taxa investigated (25 species including two varieties of 1 species and three varieties of another) were colonized by AM fungi. The mean proportion of root length colonized was 56% (range 10-95%). Vesicles were observed in 27 and hyphal coils in 26 of the 28 plant taxa. Mycorrhizas were of the Paristype or intermediate-type, with no Arum-type mycorrhizas observed. Species richness of AM fungi varied from 3 to 15 and spore density from 46 to 1,499 per 100 g rhizosphere soil. Of 33 AM fungal taxa in five genera isolated and identified, 18 belonged to Glomus, 9 to Acaulospora, 1 to Entrophospora, 2 to Gigaspora, and 3 to Scutellospora. Acaulospora and Glomus were the dominant genera identified. Glomus claroideum was the taxon most commonly isolated, with a frequency of occurrence of 56.5% and relative abundance of 10.4%. A positive correlation was found between percentage of root length colonization and species richness. However, there was no correlation between spore density and percentage of root length colonized by AM fungi.

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Section: Assessment Of Am Colonizationmentioning

confidence: 99%

Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan island, China

et al. 2005

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“…After 4 weeks, roots were carefully washed away from soil particles under tap water and stained according to Koske & Gemma (1989). Roots were cut in 1-cm segments and inoculum potential was estimated by calculating the percentage of 120 root segments bearing arbuscules, internal hyphae or vesicles, according to the method proposed by Biermann & Linderman (1981).…”

Section: Methodsmentioning

confidence: 99%

Occurrence of arbuscular mycorrhizal fungi in soils of early stages of a secondary succession of Atlantic Forest in South Brazil

et al. 2006

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RESUMO -(Ocorrência de fungos micorrízicos arbusculares em solos de estádios iniciais de uma sucessão secundária da Floresta Atlântica no Sul do Brasil). A diversidade de fungos micorrízicos arbusculares (FMAs) e o potencial de inóculo micorrízico foram determinados em estádios de sucessão secundária da Floresta Atlântica. Dentro de cada estádio -pioneiro, capoeirinha e capoeirão -quatro transectos foram estabelecidos e três amostras de solo foram obtidas por transecto. A comunidade vegetal foi dominada por Pteridium aquilinium no estádio pioneiro e Dodonaea viscosa e P. aquilinium foram co-dominantes na capoeirinha. No capoeirão, Miconia cinnamomifolia foi dominante seguida por Euterpe edulis. O número total de esporos foi significativamente maior na capoeirinha do que nos outros estádios, embora o número de esporos viáveis permaneceu constante entre os estádios sucessionais. Acaulosporaceae e Glomeraceae foram as famílias predominantes e perfizeram 83% do total de esporos recuperados. Dos 18 morfotipos de esporos recuperados, 10 foram alocados para espécies conhecidas, com uma espécie de Acaulospora sp. e uma de Glomus sp. sendo os esporuladores predominantes e presentes em todas as amostras. O indíce de diversidade de Simpson e a eqüitabilidade para as espécies de FMAs não diferiram significativamente entre os estádios sucessionais e a riqueza específica de FMAs foi negativamente correlacionada com a riqueza específica vegetal. O solo da Capoeirinha apresentou o maior potencial de inóculo (37%). A dominância da comunidade micorrízica por poucos esporuladores e a relação entre diversidade fúngica e vegetal são discutidos.Palavras-chave: Glomerales, fungos micorrízicos arbusculares, Floresta Atlântica, sucessão vegetal secundária, diversidade de espécies ABSTRACT -(Occurrence of arbuscular mycorrhizal fungi in soils of early stages of a secondary succession of Atlantic Forest in South Brazil). Arbuscular mycorrhizal fungi (AMF) species diversity and mycorrhizal inoculum potential were assessed in areas representative of stages of secondary succession in the Brazilian Atlantic Rain Forest. Within each stage -pioneer, 'capoeirinha' and 'capoeirão'-four transects were established and three soil samples were taken along each transect. The plant community was dominated by Pteridium aquilinium in the pioneer stage, while Dodonaea viscosa and P. aquilinium were co-dominants in the 'capoeirinha' stage. In capoeirão, Miconia cinnamomifolia was dominant followed by Euterpe edulis. Total spore number per 100 g soil was significantly larger in the 'capoeirinha' stage than in the other stages, although the number of viable spores was similar among stages. Acaulosporaceae and Glomeraceae were the predominant families accounting for 83% of the total spores recovered. Of the 18 spore morphotypes, 10 were allocated to known species, with Acaulospora sp. and Glomus sp. being the dominants recovered in all samples. Simpson's index of diversity and evenness for AMF species were not significantly different among the successional st...

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“…Dry (60°C) weight of tubers was recorded after the second growing cycle (2003). All roots were removed from tubers in all treatments prior to curing or drying, cut into 1-cm sections, cleared, and stained, and quantified for percent root length colonized by AMF as in Biermann and Linderman (1980). Chemical analysis of randomly selected tubers (n=8) was conducted at the end of the first growing cycle (2002).…”

Section: Measurementsmentioning

confidence: 99%

Phosphorus Supply Alters Tuber Composition, Flower Production, and Mycorrhizal Responsiveness of Container-grown Hybrid Zantedeschia

Schreiner

2006

Plant Soil

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One-year old tubers of two hybrid calla lily (calla) cultivars (Zantedeschia 'Pot of Gold' and 'Majestic Red') were inoculated with the arbuscular mycorrhizal fungus (AMF), Glomus intraradices, or not, and grown at three different rates of phosphorus (P) supply to asses the effects of AMF-inoculation on plant development (time of shoot emergence and flowering), flowering (number, length and rate of flowering), and tuber biomass and composition over two growing cycles (2002, 2003). Tubers and flowers of calla responded differently to AMF inoculation. Differences in mycorrhizal responsiveness between cultivars was related to differences in P requirements for flower and tuber production, and the influence of P supply on resource allocation to different reproductive strategies. Inoculation increased shoot production and promoted early flowering, particularly in 2003. Inoculated plants also produced larger tubers than non-inoculated plants, but only increased the number of flowers per plant in 2003. High P supply also increased tuber biomass, but decreased the number of flowers per plant in 2002. Plants grown at a moderate P-rate, produced the most flowers in 2003. For 'Majestic Red', benefits from AMF were primarily in terms of tuber yield and composition, and AMF effects on marketable flower production could potentially have negative impact on production strategies for growers. Inoculation of 'Pot of Gold' primarily influenced flower production and aspects of tuber quality that caused detectable enhancement of tuber yield and flowering in the second growing cycle following inoculation (2003). The results of this study show that the responses of calla to AMF are partially a function of how nutrient supply alters resource allocation to sexual and vegetative reproduction. Whether AMF-induced changes in resource allocation to flowering and tubers significantly alters commercial productivity and quality of calla depends on the crop production goals (e.g. tubers, cut flowers or potted plants).

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QUANTIFYING VESICULAR‐ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION ^*

Cited by 380 publications

References 10 publications

Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan island, China

Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan island, China

Occurrence of arbuscular mycorrhizal fungi in soils of early stages of a secondary succession of Atlantic Forest in South Brazil

Phosphorus Supply Alters Tuber Composition, Flower Production, and Mycorrhizal Responsiveness of Container-grown Hybrid Zantedeschia

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QUANTIFYING VESICULAR‐ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION *

Cited by 380 publications

References 10 publications

Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan island, China

Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan island, China

Occurrence of arbuscular mycorrhizal fungi in soils of early stages of a secondary succession of Atlantic Forest in South Brazil

Phosphorus Supply Alters Tuber Composition, Flower Production, and Mycorrhizal Responsiveness of Container-grown Hybrid Zantedeschia

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QUANTIFYING VESICULAR‐ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION ^*