Quantifying hyperoxia-mediated damage to mammalian respiratory cilia-driven fluid flow using particle tracking velocimetry optical coherence tomography

Gamm, Ute A.; Huang, Brendan K.; Syed, Mansoor Ali; Zhang, Xuchen; Bhandari, Vineet; Choma, Michael A.

doi:10.1117/1.jbo.20.8.080505

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…Previous studies in nontransplant situations have demonstrated that prolonged exposure to positive pressure ventilation significantly reduces tracheal CBF and overall MCC 20,21 . Similarly, exposures to high normobaric oxygen concentration lead to decreases in CBF and overall impairment in fluid flow and cellular damage to mammalian tracheal epithelium 22,23 …”

Section: Discussionmentioning

confidence: 97%

Airway cilia recovery post lung transplantation

Suryadinata

Levin

Holsworth

et al. 2021

Immunity Inflam & Disease

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Background Normally functioning airway cilia is essential for efficient mucociliary clearance to protect the airway from various insults. Impaired clearance may lead to increased risk of infections and progressive lung damage. Significant morbidity in the immediate post lung transplantation period is associated with airway infection, which we hypothesize may be caused by impaired cilia function. Methods Airway cilia beating pattern (CBP) and frequency (CBF) were studied on brushing samples taken from above and below the transplant anastomosis of adult lung transplant recipients (n = 20) during routine bronchoscopies at 6, 12, and 26 weeks posttransplant. Bronchoaveolar Lavage (BAL) samples were also collected at each time points. Results At 6 weeks posttransplant (n = 16), CBP from the donated lung showed reduced beating amplitude with the overall CBF 2.28 Hz slower than the patients' native upper airway cilia (median ± SIQR: 5.36 ± 0.93 Hz vs. 7.64 ± 0.92 Hz, p value < .001). At 12 weeks (n = 16), donor lungs CBP showed recovery with the difference in CBF reduced to 0.74 Hz (6.36 ± 1.46 Hz vs. 7.10 ± 0.86 Hz, p value < .05). Impaired cilia function was not associated with positive BAL cultures. Conclusion Reduced cilia function is evident in the first 12 weeks post lung transplant, with both CBP and CBF returning to levels of function indistinguishable to the patients' upper airway cilia beyond this time.

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Section: Discussionmentioning

confidence: 97%

Airway cilia recovery post lung transplantation

Suryadinata

Levin

Holsworth

et al. 2021

Immunity Inflam & Disease

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“…Jonas et al proposed an OCT based particle tracking velocimetry (PTV) to characterize the cilia driven flow on the ciliated epithelium of Xenopus tropicalis embryos . Follow up studies by the same group further expanded the measurement to three dimensions .…”

Section: Introductionmentioning

confidence: 99%

Ex vivo visualization of human ciliated epithelium and quantitative analysis of induced flow dynamics by using optical coherence tomography

et al. 2017

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Background and Objective Cilia-driven mucociliary clearance is an important self-defense mechanism of great clinical importance in pulmonary research. Conventional light microscopy possesses the capability to visualize individual cilia and its beating pattern but lacks the throughput to assess the global ciliary activities and flow dynamics. Optical coherence tomography (OCT), which provides depth-resolved cross-sectional images, was recently introduced to this area. Materials and Methods Fourteen de-identified human tracheobronchial tissues are directly imaged by two OCT systems: one system centered at 1300 nm with 6.5 μm axial resolution and 15 μm lateral resolution, and the other centered at 800 nm with 2.72 μm axial resolution and 5.52 μm lateral resolution. Speckle variance images are obtained in both cross-sectional and volumetric modes. After imaging, sample blocks are sliced along the registered OCT imaging plane and processed with hematoxylin and eosin (H&E) stain for comparison. Quantitative flow analysis is performed by tracking the path-lines of microspheres in a fixed cross-section. Both the flow rate and flow direction are characterized. Results The speckle variance images successfully segment the ciliated epithelial tissue from its cilia-denuded counterpart, and the results are validated by corresponding H&E stained sections. A further temporal frequency analysis is performed to extract the ciliary beat frequency (CBF) at cilia cites. By adding polyester microspheres as contrast agents, we demonstrate ex vivo imaging of the flow induced by cilia activities of human tracheobronchial samples. Conclusion This manuscript presents an ex vivo study on human tracheobronchial ciliated epithelium and its induced mucous flow by using OCT. Within OCT images, intact ciliated epithelium is effectively distinguished from cilia-denuded counterpart, which serves as a negative control, by examining the speckle variance images. The cilia beat frequency is extracted by temporal frequency analysis. The flow rate, flow direction and particle throughput are obtained through particle tracking. The availability of these quantitative parameters provides us with a powerful tool that will be useful for studying the physiology, pathophysiology and the effectiveness of therapies on epithelial cilia function, as well as serve as a diagnostic tool for diseases associated with ciliary dysmotility.

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