Quantification of αS1-casein in breast milk using a targeted mass spectrometry-based approach

Altendorfer, Irina; König, Simone; Braukmann, Achim; Saenger, Thorsten; Bleck, Ellen; Vordenbäumen, Stefan; Kubiak, Anna; Schneider, Matthias; Jose, Joachim

doi:10.1016/j.jpba.2014.10.034

Cited by 19 publications

(28 citation statements)

References 18 publications

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“…Traditionally, chromatographic or electrophoretic methods have been used to profile major BM proteins [33,34,35] whereas immuno-based approaches, i.e., ELISA or antibody arrays, were the method of choice for quantitative analysis of individual proteins [36,37,38]. More recently, targeted LC-MS techniques have been developed to separate and quantify specific milk proteins [39]. The low throughput nature of these methodologies is often the limiting factor for application to larger numbers of samples.…”

Section: Discussionmentioning

confidence: 99%

Temporal Changes of Protein Composition in Breast Milk of Chinese Urban Mothers and Impact of Caesarean Section Delivery

et al. 2016

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Human breast milk (BM) protein composition may be impacted by lactation stage or factors related to geographical location. The present study aimed at assessing the temporal changes of BM major proteins over lactation stages and the impact of mode of delivery on immune factors, in a large cohort of urban mothers in China. 450 BM samples, collected in three Chinese cities, covering 8 months of lactation were analyzed for α-lactalbumin, lactoferrin, serum albumin, total caseins, immunoglobulins (IgA, IgM and IgG) and transforming growth factor (TGF) β1 and β2 content by microfluidic chip- or ELISA-based quantitative methods. Concentrations and changes over lactation were aligned with previous reports. α-lactalbumin, lactoferrin, IgA, IgM and TGF-β1 contents followed similar variations characterized by highest concentrations in early lactation that rapidly decreased before remaining stable up to end of lactation. TGF-β2 content displayed same early dynamics before increasing again. Total caseins followed a different pattern, showing initial increase before decreasing back to starting values. Serum albumin and IgG levels appeared stable throughout lactation. In conclusion, BM content in major proteins of urban mothers in China was comparable with previous studies carried out in other parts of the world and C-section delivery had only very limited impact on BM immune factors.

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Section: Discussionmentioning

confidence: 99%

Temporal Changes of Protein Composition in Breast Milk of Chinese Urban Mothers and Impact of Caesarean Section Delivery

et al. 2016

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Section: Methodsmentioning

confidence: 99%

“…Breast milk samples of the first week postpartum were treated and subjected to LC‐MS analysis as described before with the exception that Synapt G2 Si instrument coupled to M‐Class (Waters Corp.) was used. Dig5 aliquots (see), which had been stored at –20°C, were analyzed. For reversed‐phase LC, gradients (30 min, flow rate 0.3 μL/min, 0.2 and 0.5 μL injections) were run from 97% aqueous solvent A (0.1% formic acid) to 50% organic solvent B (0.1% formic acid, acetonitrile) using a 1.8 μm ACQ M‐Class HSS T3 C18 75 μm x 150 mm column with a ACQ M‐Class Sym 100 C18 5 μm, 100 Å, 20 mm trap column.…”

Section: Methodsmentioning

confidence: 99%

“…After an initial screening, the analysis was limited to targeted measurements of non‐, mono‐ and di‐phosphorylated tryptic peptides (Table ). It was performed as described earlier . For the determination of the relative degree of phosphorylation the peak intensities of fragment ions (Table , sum of six scans) were used.…”

Section: Methodsmentioning

confidence: 99%

“…More recently our group has also shown that the phosphorylation of αS1‐casein by casein kinase (CK) 2 completely abolished binding to TLR‐4 and proinflammatory cytokine induction. Conclusively, we were interested in the concentration of αS1‐casein in breast milk . Here, we aimed to elucidate, which amino acid residues are phosphorylated in breast milk.…”

Section: Introductionmentioning

confidence: 99%

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Ser71 of αS1‐Casein is Phosphorylated in Breast Milk—Evidence from Targeted Mass Analysis

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et al. 2017

Molecular Nutrition Food Res

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Recent applications of CE‐ and HPLC‐MS in the analysis of human fluids

et al. 2015

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The present review intends to cover the literature on the use of CE-/LC-MS for the analysis of human fluids, from 2010 until present. It has been planned to provide an overview of the most recent practical applications of these techniques to less extensively used human body fluids, including, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate, tear fluid, breast fluid, amniotic fluid, and cerumen. Potential pitfalls related to fluid collection and sample preparation, with particular attention to sample clean-up procedures, and methods of analysis, from the research laboratory to a clinical setting will also be addressed. While being apparent that proteomics/metabolomics represent the most prominent approaches for global identification/quantification of putative biomarkers for a variety of human diseases, evidence is also provided of the suitability of these sophisticated techniques for the detection of heterogeneous components carried by these fluids.

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Quantification of αS1-casein in breast milk using a targeted mass spectrometry-based approach

Cited by 19 publications

References 18 publications

Temporal Changes of Protein Composition in Breast Milk of Chinese Urban Mothers and Impact of Caesarean Section Delivery

Temporal Changes of Protein Composition in Breast Milk of Chinese Urban Mothers and Impact of Caesarean Section Delivery

Ser71 of αS1‐Casein is Phosphorylated in Breast Milk—Evidence from Targeted Mass Analysis

Recent applications of CE‐ and HPLC‐MS in the analysis of human fluids

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