Quantification of withaferin-A and withanolide-A in diploid (n = 12) and tetraploid cytotypes (n = 24) of “Rassbhary”, Physalis angulata L

Abstract

During the present study an analytical method based on reverse-phase high performance liquid chromatography photodiode array detection method was developed for simultaneous determination of withaferin-A and withanolide-A in plant parts of two cytotypes (diploid n=12 & tetraploid n=24) of Physalis angulata. All the plant parts were extracted in different solvent solutions i.e., acidic [HCl] methanol, methanol, n-hexane, chloroform. Both the compounds were comparatively analyzed. The results revealed that tetraploid cytotype (n=24) showed the higher composition of both the reference compounds. The HPLC separation process was performed on Attantis T3 Column (5µm pore size, 4.6mm×250mm) with diode-array detection held at 25°C utilizing, acetonitrile and water as mobile phase at flow rate of 0.8mL/min in isocratic elution. Calibration curves produced satisfactory linear regression (r 2 >0.999) for each examined sample. The method is simple, rapid and provides better resolution can be easily applied to the quantitative analyses of withanolides in plant matrices.

Evolutionary Fixed Potential Agronomic Traits in Polysomic Polyploidy Plants with Special Reference to Potato

“…Tetraploid cytotypes of Physalis angutala Linn. from Rajasthan increased palmitic acid, linoleic acid and linolenic acid [61]. In the last century, many plant breeders have given objects based on the outstanding advantages of polyploids.…”

Development of Chloroplast Microsatellite Markers and Evaluation of Genetic Diversity and Population Structure of Cutleaf Groundcherry (Physalis angulata L.) in China

“…In many countries, such as China, Indonesia, Peru, Mexico, and Brazil, P. angulata is often used to treat a variety of illnesses, including dermatitis, tracheitis, impaludism, rheumatism, hepatitis, and analogous conditions [7][8][9][10]. Recently, increasing attention has been paid to the phytochemical and pharmacological aspects of P. angulata, and a variety of bioactive steroids with antitumor activities, including physagulins (A−Q), physangulidines (A−C), withangulatins (A−I), physalins (B, D, F−H), and withaminimin, have been isolated from the species [4,5,11]. Due to their important medicinal value, P. angulata plants have been widely cultivated in some regions of China for decades.…”

Therapeutic potential of withaferin A against non-small cell lung cancer.

“…One major hindrance to the study of the pharmacokinetics of WFA has been the lack of appropriate analytical methods. In literature, there are a few HPLC and HPLC-ESI-MS/MS methods for the detection of have been published [125][126][127]. It has been reported that these methods could detect and quantify WFA in plasma up to a lower limit of 50 ng/mL.…”