Quantification of vitamin D3 and 25-hydroxyvitamin D3 in food – The impact of eluent additives and labelled internal standards on matrix effects in LC-MS/MS analysis

Švarc, Petra Ložnjak; Barnkob, Line Lundbæk; Jakobsen, Jette

doi:10.1016/j.foodchem.2021.129588

Cited by 13 publications

(7 citation statements)

References 31 publications

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“…To certify the value of AFB 1 in the CRMs, the matrix effect (ME) of the measurement method should be overcome appropriately. Therefore, an isotope-labeled internal standard method has been adopted to overcome the ME of the LC-MS/MS technique. − According to previous reports, matrix effect factors, ME α and ME β , are used to estimate the ME of AFB 1 and isotope-labeled AFB 1 , respectively. Then, the correction factor (θ) of the IDMS method, which is equal to the ratio of ME α and ME β , is the parameter to evaluate the ME of measurement results. , In the study, the calculated ME α and ME β values were 2.43 and 2.42, respectively.…”

Section: Resultsmentioning

confidence: 99%

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Zhao,

Li,

et al. 2024

ACS Food Sci. Technol.

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Aflatoxin B 1 in a peanut oil matrix certified reference material (CRM, GBW10176) has been issued for accurate determination of aflatoxin B 1 in real samples. This matrix CRM was prepared from commercial peanut oils naturally contaminated by aflatoxin B 1 , with the aid of a liquid−liquid extraction process. Specially, the certified value of CRM was determined using two individual approaches, liquid chromatography−isotope dilution tandem mass spectrometry and standard addition liquid chromatography−fluorescence detection techniques. More importantly, assessments of homogeneity and short-and long-term stabilities confirmed that the GBW10176 CRM was sufficiently homogeneous between packages or within package, and it could be stable up to 7 days stored below 50 °C or 18 months at 4 °C. The metrological traceability of GBW10176 was ensured by the application of pure aflatoxin B 1 (GBW10172). Its uncertainty was evaluated with the contributions from the characterization, homogeneity, and stability. Resultantly, the certified value of the aflatoxin B 1 CRM (GBW10176) was 23.4 μg/kg with an uncertainty of 3.1 μg/kg (k = 2).

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Section: Resultsmentioning

confidence: 99%

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Zhao,

Li,

et al. 2024

ACS Food Sci. Technol.

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“…This finding indicates that appropriate additives can reduce the need for numerous ILIS, presenting a substantial advantage in method development. Other studies have incorporated additives into the mobile phase, which imposes certain limitations regarding concentration and compatibility with mass spectrometry and the chromatography column. ,− The approach described herein not only simplifies and reduces costs (the additive is directly added to the sample rather than the mobile phase) but also offers greater flexibility with reversed-phase columns and mass spectrometry. This flexibility stems from their inert nature, high solubility in the mobile phase, and ease of rapid removal to waste.…”

Section: Resultsmentioning

confidence: 99%

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Orlando,

Augusti,

Nori de Macedo

et al. 2023

ACS EST Water

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This manuscript describes a novel and simple strategy for optimizing the determination of contaminants in groundwater using online solid-phase extraction (SPE) coupled with ultrahigh-performance liquid chromatography (UHPLC) and tandem mass spectrometry (MS/MS). The methodology optimization process considered several factors: analyte sorption in the syringe filter material, SPE conditions for effective cleanup, maximum injection volume, and spiking samples with additives to mitigate matrix effects. Matrix effects posed the greatest challenge, which was addressed by adding a few microliters of additives (citric acid and hydroxylamine) directly into the groundwater samples. This simple yet advantageous approach was also demonstrated with UHPLC coupled to high-resolution mass spectrometry without employing SPE. Under the optimized conditions, ten contaminants were evaluated, yielding excellent results at detected levels ranging from parts per billion (ppb) to parts per trillion (ppt), even without using isotopically labeled internal standards. These findings highlight the importance of investigating possible experimental issues that have a critical influence on the performance of analytical protocols. Finally, this work provides valuable insights for other researchers working on LC-MS/MS methods for determining organic contaminants in groundwater.

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“…Vitamin D 3 analysis was performed as previously described [ 18 , 21 ]. In short, 0.2 g of previously homogenized pork cracklings, or 0.3 g of raw pork rind, was weighed together with 0.2 g of sodium ascorbate.…”

Section: Methodsmentioning

confidence: 99%

“…The vitamin D 3 was further extracted with 20% ethyl acetate in n -heptane by liquid-liquid extraction, cleaned by solid-phase extraction (HybridSPE-phospholipid) and derivatized with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) for 5 min in the darkness. The vitamin D 3 was analyzed by LC-MS/MS [ 21 ]. With the exception of methanol used for LC-MS/MS, all chemicals used in the method were of analytical quality.…”

Section: Methodsmentioning

confidence: 99%

Bio-Fortified Pork Cracklings with UVB LED Tailored Content of Vitamin D3

Švarc

Rahimi

Tønnesen

et al. 2022

Foods

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Since few foods are naturally rich in vitamin D, novel food products with a high content of vitamin D are needed to decrease the prevalence of vitamin D deficiency. Pork cracklings are Danish snacks with high contents of protein and fat. They are consumed mostly during wintertime when sun exposure cannot fulfil human needs for vitamin D3. Pork cracklings were produced in an industrially friendly manner from UVB LED illuminated pork rind, using a combination of sous vide (85 °C, 60 min) and roasting in the oven (200 °C, 20 min). Thermal processing resulted in a significant loss of vitamin D3 (>90%). Thus, the process was optimized by the UVB exposure of pork cracklings, i.e., after thermal processing. The produced pork cracklings had a vitamin D3 level of ~10 µg/100 g, with a possibility of tailoring its final content. Furthermore, the fat content at 15–20% was a reduction of 50% compared to marketed products in 2021. No significant difference was found in the content of vitamin D3 during 31 days of storage in the air. A consumer preference test (n = 53) indicated that >80% of participants liked the product and saw its potential as a new food source of vitamin D3.

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Quantification of vitamin D3 and 25-hydroxyvitamin D3 in food – The impact of eluent additives and labelled internal standards on matrix effects in LC-MS/MS analysis

Cited by 13 publications

References 31 publications

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Bio-Fortified Pork Cracklings with UVB LED Tailored Content of Vitamin D3

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Quantification of vitamin D3 and 25-hydroxyvitamin D3 in food – The impact of eluent additives and labelled internal standards on matrix effects in LC-MS/MS analysis

Cited by 13 publications

References 31 publications

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B1 in Peanut Oils

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B1 in Peanut Oils

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Bio-Fortified Pork Cracklings with UVB LED Tailored Content of Vitamin D3

Contact Info

Product

Resources

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Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils