Quantification of viable cells of Clavibacter michiganensis subsp. sepedonicus in digester material after heat treatment by TaqMan® BIO-PCR

Kaemmerer, D.

doi:10.1007/bf03356280

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…Interestingly, the same potato heel end extract that is prepared to be used for ring rot test can also be used for detection of the other bacterial pathogens of potato, that is, soft rot pectobacteria and the brown rot pathogen R. solanacearum. Culture-based enrichment of the bacterial population followed by specific PCR or TaqMan, a technique known as (TaqMan) BIO-PCR, was found to be highly sensitive for screening of potato tuber extracts for C. sepedonicus (Kaemmerer, 2009;Schaad et al, 1999).…”

Section: Detectionmentioning

confidence: 99%

“…solanacearum . Culture‐based enrichment of the bacterial population followed by specific PCR or TaqMan, a technique known as (TaqMan) BIO‐PCR, was found to be highly sensitive for screening of potato tuber extracts for C. sepedonicus (Kaemmerer, 2009 ; Schaad et al, 1999 ).…”

Section: Isolation Detection and Identification Of The Pathogenmentioning

confidence: 99%

“…Furthermore, over‐reliance on copper‐based chemicals in agriculture has resulted in environmental and groundwater pollution (Lamichhane et al, 2018 ). Heat treatments of potato to eliminate C. sepedonicus from tubers have been investigated with mixed results (Kaemmerer, 2009 ). Recycling of plant materials by means of composting and subsequent application of the produced humus to the environment has become a common practice, with a risk of spreading C. sepedonicus .…”

Section: Managementmentioning

confidence: 99%

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Bacterial ring rot of potato caused by Clavibacter sepedonicus: A successful example of defeating the enemy under international regulations

Osdaghi

Wolf

Abachi

et al. 2022

Molecular Plant Pathology

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Background Bacterial ring rot of potato (Solanum tuberosum) caused by the gram‐positive coryneform bacterium Clavibacter sepedonicus is an important quarantine disease threatening the potato industry around the globe. Since its original description in 1906 in Germany, management of ring rot has been a major problem due to the seedborne nature (via seed tubers not true seeds) of the pathogen allowing the bacterium to be transmitted long distances via infected tubers. Disease symptoms On growing potato plants: interveinal chlorosis on leaflets leading to necrotic areas and systemic wilt. On infected tubers: vascular tissues become yellowish brown with a cheesy texture due to bacterial colonization and decay. Host range Potato is the main host of the pathogen, but natural infection also occurs on eggplant, tomato, and sugar beet. Taxonomic status of the pathogen Class: Actinobacteria; Order: Actinomycetales; Family: Microbacteriaceae; Genus: Clavibacter; Species: Clavibacter sepedonicus (Spieckermann and Kotthoff 1914) Li et al. 2018. Synonyms (nonpreferred scientific names) Aplanobacter sepedonicus; Bacterium sepedonicum; Corynebacterium sepedonicum; Corynebacterium michiganense pv. sepedonicum; Clavibacter michiganensis subsp. sepedonicus. Microbiological properties Gram‐positive, club‐shaped cells with creamy to yellowish‐cream colonies for which the optimal growth temperature is 20–23°C. Distribution Asia (China, Japan, Kazakhstan, Nepal, North Korea, Pakistan, South Korea, Uzbekistan, the Asian part of Russia), Europe (Belarus, Bulgaria, Czech Republic, Estonia, Finland, Georgia, Germany, Greece, Hungary, Latvia, Lithuania, Norway, Poland, Romania, European part of Russia, Slovakia, Spain, Sweden, Turkey, Ukraine), and North America (Canada, Mexico, USA). Phytosanitary categorization CORBSE: EPPO A2 list no. 51. EU; Annex designation I/A2.

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Section: Detectionmentioning

confidence: 99%

Section: Isolation Detection and Identification Of The Pathogenmentioning

confidence: 99%