Quantification of Thrombocyte Growth Factors in Platelet Concentrates Produced by Discontinuous Cell Separation

Abstract

Platelet concentrates (PC) are increasingly used to increase bone regeneration in pre-prosthetic surgery. Although it is generally appreciated that certain growth factors (PDGF, TGF, EGF, and ECGF) are present in thrombocyte preparations, relatively little is known about these components in quantitative terms. The study reported here analysed the amounts of growth factors in PC produced under standard conditions from healthy volunteers. All the blood samples (237 in total) were analysed using Quantikine ELISA kits (R and D). The mean +/- SD platelet count in whole blood from these donors was 262,000+/-58,000/microl, while in PC produced by discontinuous cell separation it was 1.419,000+/-333,000/microl. The mean growth factor concentrations in PC preparations in ng/ml were as follows: PDGF-AB 125+/-55 ng/ml; TGF-beta1 221+/-92 ng/ml; IGF-I 85+/-25 ng/ml; PDGF-BB 14+/-9 ng/ml; TGF-beta2 0.4+/-0.3 ng/ml. These growth factor concentrations typically covered a 3-10 fold range: PDGF-AB 29-277ng/ml; PDGF-BB 2-33ng/ml; TGF-beta1 32-397ng/ml; TGF-beta2 0.1-1.2 ng/ml; IGF-I 40-138 ng/ml. Platelet counts in PC were slightly higher for women (Mann-Whitney Test all p < 0.001) than for men, while the concentrations of growth factors in PC exhibited no gender-related difference of any statistical significance.

Biochemical characteristics of the equine Autologous Conditioned Plasma® (ACP)

“…Bei der Zentrifugation mit 1300 U/min x 6 min lagen die GF-Werte deutlich darunter. Argüelles et al (2006) (Anitua 1999, Weibrich et al 2002.…”

“…Diese Aktivierung geschieht durch spezifische Thrombozytenagonisten (Thrombin, Kollagen, Kalzium, ADP, Thromboxan A2, Noradrenalin, Serotonin), aber auch durch mechanischen "Scherkontakt" (Schrör 2001, Eppley et al, 2004. Verschiedene Studien konnten zeigen, dass die Konzentration an GF in TZK direkt proportional zur TZ-Konzentration ist (Argüelles et al 2006, Weibrich et al 2002. Zwei wichtige GF für die Gewebsregeneration stellen PDGF-BB (plateletderived growth factor BB) und TGF-b 1 (transforming growth factor b 1 ) dar.…”

Is there any association between mycosis fungoides and Th-1, Th-2 cytokines and transforming growth factor-beta 1 gene polymorphisms?

“…These growth factors and cytokines within PRP speed up extracellular matrix (ECM) formation resulting in tissue regeneration. The interaction between these bioactive proteins and receptors has a key role in the process of wound healing 7,8 . PRP is used in the treatment of chronic cutaneous and soft tissue ulcerations and especially in periodontal and oral surgery, maxillofacial surgery, orthopedic and traumatic surgery, cosmetic and plastic surgery, spinal surgery, heart by-pass surgery, and in burns [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] .…”

Componentes ricos em plaquetas na reparação de afecções tendo-ligamentosas e osteo-articulares em animais

“…A degranulação plaquetária propicia a liberação das seguintes substâncias: fator de crescimento de transformação beta (TGF-ß), fibrinogênio, fator de crescimento derivado da plaqueta (PDGF), fator de crescimento epidermal (EGF), fator de crescimento de transformação alfa (TGF-α), fator de crescimento vascular endotelial (VEGF), tromboplastina plaquetária, cálcio, serotonina e enzimas hidrolíticas (HARRISON & CRAMER, 1993 (KUBOTA et al, 2004) e o semelhante à insulina I (IGF-I) (WEIBRIC et al, 2002) também estão presentes nas plaquetas.…”