Quantification of the Mycotoxin Deoxynivalenol (DON) in Sorghum Using GC-MS and a Stable Isotope Dilution Assay (SIDA)

McMaster, Nicole; Acharya, Bhupendra; Harich, Kim; Grothe, Jan Hendrik; Mehl, H. L.; Schmale, David G.

doi:10.1007/s12161-019-01588-3

Cited by 18 publications

(7 citation statements)

References 27 publications

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“…The FDK was calculated by counting shriveled or scabby seeds in 100‐seed random subsamples in five replicates from each location and entry. A representative subsample was ground to meal, and DON was quantified using gas chromatography–mass spectrometry (GC–MS) in the Schmale Laboratory at Virginia Tech using standard protocols (Khatibi et al., 2014; McMaster et al., 2019; Tacke & Casper, 1996).…”

Section: Methodsmentioning

confidence: 99%

Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’

Carpenter

Wright

Malla³

et al. 2020

Crop Science

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Use of genetic resistance is one of the most important strategies to manage the devastating disease Fusarium head blight (FHB) in wheat. Numerous quantitative trait loci (QTL) having varying effects on reducing FHB and the mycotoxin deoxynivalenol (DON) accumulation have been reported from Asian, European, or distant sources such as wild relatives of wheat (Triticum aestivum L.). However, coming from nonadapted backgrounds, the incorporation of such QTL into regional breeding programs has often resulted in the simultaneous transfer of other undesirable traits. Therefore, it is important to identify, characterize, and deploy sources of genetic resistance that do not suffer from poor adaptability and/or linkage drag. In the present work, QTL associated with FHB resistance in a high‐yielding, moderately resistant soft red winter wheat cultivar ‘Jamestown’ were mapped and validated. The QTL mapping was done using a recombinant inbred line (RIL) population of Pioneer ‘25R47’ × Jamestown having 186 individuals. Phenotyping over 2 yr at three locations, and genotyping using the 90K single nucleotide polymorphism (SNP) platform identified two new QTL, named QFHB.vt‐1B.1 and QFHB.vt‐1B.2, on the chromosome 1B long arm. The QTL contributed to FHB incidence, FHB severity, Fusarium‐damaged kernels, and DON content. Independent mapping of these QTL using two additional RIL populations of FG95195 × Jamestown (170 RILs) and Jamestown × LA97113UC‐124 (77 RILs) validated their stability and effectiveness in different genetic backgrounds. Kompetitive allele specific polymerase chain reaction (KASP) assays were developed using linked SNPs for marker‐assisted selection of the QTL. These QTL are being used in breeding programs to develop FHB‐resistant, high‐yielding varieties.

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Section: Methodsmentioning

confidence: 99%

Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’

Carpenter

Wright

Malla³

et al. 2020

Crop Science

Self Cite

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“…Various adsorbent materials have been commercially available, including charcoal, silica, modified silica, Florisil (magnesium silicate), silica-based octadecyl silane (C18), polymers, ion-exchange resins, Celite, aluminum oxide, charcoal–alumina, NH 2 , and the selection of the proper sorbent is mainly governed by several parameters, including the nature of analytes, food matrix, extraction solvent and co-extractive interference components that could be existing in the sample extract [ 166 , 203 , 204 ]. C18, Celite, Florisil, aluminum oxide, and charcoal–alumina are commonly employed for the extraction of trichothecenes from various food materials [ 19 , 39 , 205 ]. Zhao et al [ 19 ] established a simple and reliable methodology for the analysis of type B trichothecenes and DON-3G in animal feed by LC-MS/MS.…”

Section: Sample Preparation Methods For Analysismentioning

confidence: 99%

Type B Trichothecenes in Cereal Grains and Their Products: Recent Advances on Occurrence, Toxicology, Analysis and Post-Harvest Decontamination Strategies

Gab-Allah

Choi

Kim

2023

Toxins

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Type B trichothecenes (deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol) and deoxynivalenol-3-glucoside (DON-3G) are secondary toxic metabolites produced mainly by mycotoxigenic Fusarium fungi and have been recognized as natural contaminants in cereals and cereal-based foods. The latest studies have proven the various negative effects of type B trichothecenes on human health. Due to the widespread occurrence of Fusarium species, contamination by these mycotoxins has become an important aspect for public health and agro-food systems worldwide. Hence, their monitoring and surveillance in various foods have received a significant deal of attention in recent years. In this review, an up-to-date overview of the occurrence profile of major type B trichothecenes and DON-3G in cereal grains and their toxicological implications are outlined. Furthermore, current trends in analytical methodologies for their determination are overviewed. This review also covers the factors affecting the production of these mycotoxins, as well as the management strategies currently employed to mitigate their contamination in foods. Information presented in this review provides good insight into the progress that has been achieved in the last years for monitoring type B trichothecenes and DON-3G, and also would help the researchers in their further investigations on metabolic pathway analysis and toxicological studies of these Fusarium mycotoxins.

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“…There have been cases where mycotoxin analyses by GC-MS were not reproducible due to inconsistent recovery. According to McMaster et al, even though the standard GC-MS operating procedure from the United States Wheat and Barley Scab Initiative (USWBSI) was adopted, the detection of DON in sorghum varied during the recovery process [ 91 ]. This issue was solved by diluting the sample with an internal standard, isotope d1-DON, during the sample preparation step [ 91 ].…”

Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Ong

Pike

Tan

2021

Foods

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The presence of mycotoxins in foodstuffs and feedstuffs is a serious concern for human health. The detection of mycotoxins is therefore necessary as a preventive action to avoid the harmful contamination of foodstuffs and animal feed. In comparison with the considerable expense of treating contaminated foodstuffs, early detection is a cost-effective way to ensure food safety. The high affinity of bio-recognition molecules to mycotoxins has led to the development of affinity columns for sample pre-treatment and the development of biosensors for the quantitative analysis of mycotoxins. Aptamers are a very attractive class of biological receptors that are currently in great demand for the development of new biosensors. In this review, the improvement in the materials and methodology, and the working principles and performance of both conventional and recently developed methods are discussed. The key features and applications of the fundamental recognition elements, such as antibodies and aptamers are addressed. Recent advances in aptasensors that are based on different electrochemical (EC) transducers are reviewed in detail, especially from the perspective of the diagnostic mechanism; in addition, a brief introduction of some commercially available mycotoxin detection kits is provided.

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Quantification of the Mycotoxin Deoxynivalenol (DON) in Sorghum Using GC-MS and a Stable Isotope Dilution Assay (SIDA)

Cited by 18 publications

References 27 publications

Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’

Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’

Type B Trichothecenes in Cereal Grains and Their Products: Recent Advances on Occurrence, Toxicology, Analysis and Post-Harvest Decontamination Strategies

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

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