Quantification of the interaction between biomaterial surfaces and bacteria by 3-D modeling

Siegismund, Daniel; Undisz, Andreas; Germerodt, Sebastian; Schuster, Stefan; Rettenmayr, Markus

doi:10.1016/j.actbio.2013.09.016

Cited by 45 publications

(22 citation statements)

References 53 publications

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“…Bacterial adhesion has been related to diverse surface parameters in the literature . Among those parameters, average surface roughness has been the most studied with regard to the antiadhesive behavior of numerous substrata . As mentioned above, R a values under 0.2 µm allow for the minimization of bacterial adhesion .…”

Section: Resultsmentioning

confidence: 99%

Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials

Altmann

Karygianni

Al‐Ahmad

et al. 2017

Adv Funct Materials

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The development of long-lasting zirconia-based ceramics for implants, which are not prone to hydrothermal aging, is not satisfactorily solved. Therefore, this study is conceived as an overall evaluation screening of novel ceriastabilized zirconia-alumina-aluminate composite ceramics (ZA 8 Sr 8 -Ce11) with different surface topographies for use in clinical applications. Ceriastabilized zirconia is chosen as the matrix for the composite material, due to its lower susceptibility to aging than yttria-stabilized zirconia (3Y-TZP). This assessment is carried out on three preclinical investigation levels, indicating an overall biocompatibility of ceria-stabilized zirconia-based ceramics, both in vitro and in vivo. Long-term attachment and mineralized extracellular matrix (ECM) deposition of primary osteoblasts are the most distinct on porous ZA 8 Sr 8 -Ce11 p surfaces, while ECM attachment on 3Y-TZP and ZA 8 Sr 8 -Ce11 with compact surface texture is poor. In this regard, the animal study confirms the porous ZA 8 Sr 8 -Ce11 p to be the most favorable material, showing the highest bone-to-implant contact values and implant stability post implantation in comparison with control groups. Moreover, the microbiological evaluation reveals no favoritism of biofilm formation on the porous ZA 8 Sr 8 -Ce11 p when compared to a smooth control surface. Hence, together with the in vitro in vivo assessment analogy, the promising clinical potential of this novel ZA 8 Sr 8 -Ce11 as an implant material is demonstrated.

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Section: Resultsmentioning

confidence: 99%

Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials

Altmann

Karygianni

Al‐Ahmad

et al. 2017

Adv Funct Materials

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“…8 C ). Siegismund et al [53] showed based on mathematical modeling that in addition to amplitude roughness parameters, such as Rq, also the surface peak density as spacing surface parameter distinctively influences the interaction energies between microbial cell and materials surface. With increasing peak density, the interaction energy increases.…”

Section: Discussionmentioning

confidence: 99%

Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

et al. 2014

Self Cite

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Biomaterials-associated infections are primarily initiated by the adhesion of microorganisms on the biomaterial surfaces and subsequent biofilm formation. Understanding the fundamental microbial adhesion mechanisms and biofilm development is crucial for developing strategies to prevent such infections. Suitable in vitro systems for biofilm cultivation and bacterial adhesion at controllable, constant and reproducible conditions are indispensable. This study aimed (i) to modify the previously described constant-depth film fermenter for the reproducible cultivation of biofilms at non-depth-restricted, constant and low shear conditions and (ii) to use this system to elucidate bacterial adhesion kinetics on different biomaterials, focusing on biomaterials surface nanoroughness and hydrophobicity. Chemostat-grown Escherichia coli were used for biofilm cultivation on titanium oxide and investigating bacterial adhesion over time on titanium oxide, poly(styrene), poly(tetrafluoroethylene) and glass. Using chemostat-grown microbial cells (single-species continuous culture) minimized variations between the biofilms cultivated during different experimental runs. Bacterial adhesion on biomaterials comprised an initial lag-phase I followed by a fast adhesion phase II and a phase of saturation III. With increasing biomaterials surface nanoroughness and increasing hydrophobicity, adhesion rates increased during phases I and II. The influence of materials surface hydrophobicity seemed to exceed that of nanoroughness during the lag-phase I, whereas it was vice versa during adhesion phase II. This study introduces the non-constant-depth film fermenter in combination with a chemostat culture to allow for a controlled approach to reproducibly cultivate biofilms and to investigate bacterial adhesion kinetics at constant and low shear conditions. The findings will support developing and adequate testing of biomaterials surface modifications eventually preventing biomaterial-associated infections.

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“…On the one hand, the oral cavity is an aggressive environment. Material surface in the mouth are covered by the salivary pellicle (up to 1,000 nm thick [39]), which can also alter the nanotopography of restorative material [40], thereby greatly influence surface roughness. Mechanical stimulus, temperature, and pH conditions can also favor additional bacterial adhesion to the prostheses and alter material surface characteristics.…”

Section: Discussionmentioning

confidence: 99%

Comparison of peri-implant submucosal microbiota in arches with zirconia or titanium implant-supported fixed complete dental prostheses (IFCDP): study protocol for a randomized controlled trial

Jia

Yang

Liu

et al. 2020

Preprint

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• Background: Implant-supported prostheses are applied in edentulous patients with high success rate. However, the incidence rate of biological complications namely peri-implant mucositis and peri-implantitis increases year by year after the placement of prostheses. Pathogenic bacteria accumulated adjacent to prostheses is proved to be the main reason of biological complication. Titanium, one of the classical materials for implant-supported prostheses, performs well in the aspect of biocompatibility and ease maintenance, but is still susceptible for biofilm formation. Zirconia, emerging as an appealing substitute, not only has comparable properties, but presents different surface properties influencing oral bacteria adhesion. However, scarce scientific research proves its direct effect on oral bacteria flora. Study exploring the different effects of material properties on biofilm formation and composition might provide a clue on this topic.• Methods: The proposed study is designed as a 5-year randomized controlled trial. We plan to enroll 36 edentulous (maxilla and/or mandible) patients seeking of full-arch fixed implant-supported prostheses. The participants will be randomly divided into two groups. Group 1: participants will be restored with zirconia frameworks with ceramic veneering; Group 2: participants will be restored with titanium frameworks with acrylic resin veneering. Ten reexaminations will be completed at the end of this 5-year trial. Mucosal conditions around the implants will be recorded every six months after restoration. Peri-implant submucosal plaque will be collected at each reexamination, and bacteria flora analysis will be performed with 16S rRNA gene sequencing technology, to compare the differences in microbial diversity between two groups. X-ray examination will be applied every 12 months as a key index to evaluate the marginal bone level around implants.• Discussion: The current study aims to explore the oral microbiology of patients restored with zirconia ceramic frameworks and those restored with titanium frameworks. By evaluating and comparing the features of the microbiota and the mucosal condition around two different materials, whether zirconia to be a recommendable material for fixed implant-supported prostheses could be figured out. This study aims to provide a tangible decision-making suggestion for full arch implant-supported prosthesis in edentulous patients. • Trial registration: International Clinical Trials Registry Platform (ICTRP), ID: ChiCTR2000029470. Registered on 2 February 2020.

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Quantification of the interaction between biomaterial surfaces and bacteria by 3-D modeling

Cited by 45 publications

References 53 publications

Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials

Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials

Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

Comparison of peri-implant submucosal microbiota in arches with zirconia or titanium implant-supported fixed complete dental prostheses (IFCDP): study protocol for a randomized controlled trial

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