The paper reveals the influence exerted on the development of post vitro grape mother plants and various microzones found on the Ust-Donetsk sand massif. The differences in the microzones were in the fertility level and a number of soil properties. The considerable influence provided by the growing conditions not only on the development of plants but also on the survival rate and life expectancy of mother bushes observed during the use of mother plants for 17 years has been noted. To ensure effective land management, a detailed site study and, depending on the differences identified, the use of precision farming techniques is required before laying out the parent plants. The reason for this is that sandy soils, in general, have a high degree of heterogeneity due to physical and chemical characteristics such as flowability, susceptibility to wind transport, low organic substance content, and cation exchange capacity.