An influence of different sucrose concentrations in the culture media on the photosynthetic parameters, photosynthetic apparatus related genes expression, oxidative processes and acclimation of grape plants cultured in vitro was examined in this article. An increase of the sucrose concentration in the culture media resulted in a reduced expression of several photosynthetic genes. The most effective functioning of the photosynthetic apparatus was discovered by a decreased amount of surcose in culture media. An increase of the sucrose concentration in the culture media disrupts pigments synthesis, particularly carotenoids, which can be a cause of the secondary oxidative stress formation and grape plants growth reduction during acclimation.
при клональном микроразмножении растений in vitro не происходит изменений на генетическом уровне даже через 8 лет культивирования, что подтвер ждает надежность разработанной технологии размножения и оздоровления аборигенных сортов винограда in vitro
The results of studies on the development of culture medium for initiation grape meristems into in vitro culture in order to increase their regenerative capacity during recovery from chronic diseases are presented. In the improved medium, in comparison with analogs, the total content of macro salts was reduced and their ratio was optimized, taking into account their consumption and the characteristics of their absorption from the nutrient solution by the grape plant. In the course of testing the new culture medium, its effectiveness has been established for grape varieties from various ecological and geographical groups. A higher and more stable percentage of survival and regeneration of extremely small meristems have been established against the background of various modifications of the recipe for the traditionally used culture medium of Murashige and Skoog.
An increase in sucrose in the medium increased the content of pigments, gene expression of the photosynthetic apparatus, growth processes, and H2O2, but decreased the quantum yield of photosynthesis. With a change in the sucrose content in the medium, the expression of ABA1 increased most intensively. 30 g/l of sucrose in the medium inhibited the expression of genes involved in the formation of ABA.
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