Quantification of the allergen soy (Glycine max) in food using digital droplet PCR (ddPCR)

Mayer, Walter; Schuller, M.; Viehauser, M. C.; Hochegger, Rupert

doi:10.1007/s00217-018-3182-5

Cited by 15 publications

(9 citation statements)

References 20 publications

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“…Results showed a clear linear relationship between the amount of DNA and the copy numbers retrieved with the ddPCR assay, allowing predictions in the 450 range from 3 ng DNA (50.51 cp/µl) up to 75 ng DNA (1191.17 cp/µl). Adding too much DNA caused the positive clusters to become indistinguishable from the negative clusters, which was also reported by Mayer et al (2019). The limit of detection for the designed ddPCR assay for Atlantic salmon was set at 0.024 ng (or 0.37 cp/µl), which is comparable with the theoretical LOD 0.32 cp/µl calculated for 15000 droplets (Deprez et al, 2016).…”

Section: Identification and Quantification Of Atlantic Salmon In Reta...supporting

confidence: 70%

“…To identify species in mixed samples, species-specific assays paired with PCR (endpoint PCR, qPCR or ddPCR) can be used or next-generation amplicon sequencing can be applied, but the latter is less appropriate for quantification (Lundberg et al, 2013). At this point, there is little evidence on the correlation of DNA based quantification of a fish species in a mixed food 115 product with the percentage mentioned on a food label (Hansen et al, 2020;Laube et al, 2007;Mayer et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR)

Deconinck

Hostens²,

Taverniers³

et al. 2021

Food and Chemical Toxicology

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Section: Identification and Quantification Of Atlantic Salmon In Reta...supporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR)

Deconinck

Hostens²,

Taverniers³

et al. 2021

Food and Chemical Toxicology

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“…Considering good accuracy for a quantitative detection, recovery in the range of 80%–120% was set to be a key criterion ( Mayer et al, 2019 ). For the formulas based on whole seeds, chestnut paste samples with mung bean mass ratios in the range of 5%–60% had unsatisfied results with the recovery from 15.40% to 71.67%.…”

Section: Resultsmentioning

confidence: 99%

A quantitative detection of mung bean in chestnut paste using duplex digital PCR

Liang

Dong-wei

Dong

et al. 2022

Current Research in Food Science

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Highly manufacturing process of chestnut paste leaves a considerable space for Economically Motivated Adulteration (EMA) with cheaper ingredients such as mung bean. In this paper a novel quantitative detection of mung bean in chestnut paste using duplex digital PCR was reported. Two sets of primers and probes were designed according to mung bean and chestnut specific genomic genes suitable for duplex droplet digital PCR (ddPCR) and duplex chip digital PCR (cdPCR) to set up a mass ratio quantitative detection method for mung bean, a common alternative plant-derived ingredient in chestnut paste products. The manufacturing process of chestnut paste products was considered to establish the linear relationship formula between mass ratio and gene copy number (CN) ratio of the two ingredients. The limits of quantification for gene CN concentrations (LOQ copy ) of mung bean and chestnut were both 6 copies/μL, at the same time a mass ratio of mung bean in chestnut paste range from 5% to 80% was able to be quantified accurately to provide technical support for the identification of fraudulent substitution or adventitious contamination.

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“…To counteract potential fraud, Chen et al [54] developed a dPCR assay for the specific detection of this plant species in starch products. Finally, to meet the need for quantification of allergens, a droplet digital PCR approach was shown to be reliable and sensitive enough to quantify the very common allergen soy in food [55].…”

Section: Plant Species Traceabilitymentioning

confidence: 99%

Digital PCR: What Relevance to Plant Studies?

Morcia

Ghizzoni

Delogu³

et al. 2020

Biology

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Digital PCR (dPCR) is a breakthrough technology that able to provide sensitive and absolute nucleic acid quantification. It is a third-generation technology in the field of nucleic acid amplification. A unique feature of the technique is that of dividing the sample into numerous separate compartments, in each of which an independent amplification reaction takes place. Several instrumental platforms have been developed for this purpose, and different statistical approaches are available for reading the digital output data. The dPCR assays developed so far in the plant science sector were identified in the literature, and the major applications, advantages, disadvantages, and applicative perspectives of the technique are presented and discussed in this review.

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Quantification of the allergen soy (Glycine max) in food using digital droplet PCR (ddPCR)

Cited by 15 publications

References 20 publications

Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR)

Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR)

A quantitative detection of mung bean in chestnut paste using duplex digital PCR

Digital PCR: What Relevance to Plant Studies?

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