Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR)

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

“…In this regard, the DNA based technologies have proven to be very effective as food fraud detection tools [ 13 , 14 ] and the methodology of DNA barcoding based on the cytochrome oxidase I sequences is the most used one [ 12 , 33 , 34 , 35 , 79 ]. In the last decade new techniques such as Real Time-PCR, Single Nucleotide Polymorphisms (SNPs), Forensically Informative Nucleotide Sequencing (FINS), Loop-mediated isothermal amplification (LAMP), Droplet Digital PCR (ddPCR), High Resolution Melting Analysis (HRMA), Next Generation Sequencing (NGS) metabarcoding, have been proposed by researchers with the aim of optimizing time, costs and effectiveness of species authentication in multi-species fish products [ 80 , 81 , 82 , 83 ]. Although these techniques have been also combined with COI barcoding by providing promising approaches for high throughput species discrimination in processed seafood [ 84 , 85 , 86 ], traditional mitochondrial DNA-based methods and the PCR-RFLP in particular is still employed due to the advantages offered such as relatively cheapness, lack of technical over-complication, suitability for routine analyses [ 85 ].…”

Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM PRNP-Knockout Goat

“…Scollo et al used ddPCR to quantify olive oil DNA and to evaluate various oil DNA extractions and amplification methodologies [ 18 ]. Deconinck et al developed one species-specific ddPCR assay for semi-quantitative evaluation of the Atlantic salmon content in processed food products [ 19 ]. Köppel et al proved that compared to qPCR, the ddPCR approach has higher efficiency when quantifying samples containing trace GM contents [ 20 ].…”

“…Köppel et al proved that compared to qPCR, the ddPCR approach has higher efficiency when quantifying samples containing trace GM contents [ 20 ]. To date, only a few ddPCR assays for GM animal content in risk assessment have been reported [ 19 , 21 ].…”

A Droplet Digital PCR Based Approach for Identification and Quantification of Porcine and Chicken Derivatives in Beef

“…This technique avoids calculating copy number of DNA from intermediary cycle threshold (Ct) value that can be affected by amplification efficiency and inhibitors, thereby increasing the overall accuracy for quantifying target DNA especially at low concentrations and/or in a high background of non-target DNA [ 20 ]. Numerous studies have evidenced the applicability of ddPCR for detection and quantification of meat adulteration [ 21 , 22 , 23 , 24 , 25 ] including determination of the presence of chicken in sheep and goat meat products [ 21 ], Atlantic salmon in processed and mixed seafood products [ 22 ] and porcine-derived materials in halal food products [ 23 ].…”