Quantification of superoxide radical production in 4 vital organs of rats subjected to hemorrhagic shock

Panteli, Eleftheria S; Fligou, Fotini; Papamichail, Chrisaugi; Papapostolou, Ioannis; Zervoudakis, George; Georgiou, Christos D.; Filos, Kriton S.

doi:10.1016/j.ajem.2010.12.031

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…Oxygen-free radicals are involved in HS and subsequent resuscitation [ 21 ]. Wu et al [ 22 ] confirmed that HS and resuscitation induced significant oxidation in rat lungs, while Panteli et al [ 23 ] reported that the oxidative state of the kidneys remained unaffected after HS. Treatment with antioxidant agents after HS reduced organ oxidative stress and led to a better outcome.…”

Section: Discussionmentioning

confidence: 99%

Kaempferol pretreatment modulates systemic inflammation and oxidative stress following hemorrhagic shock in mice

Yang

He²,

Zhou

et al. 2015

Chin Med

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BackgroundKaempferol has been reported as beneficial for both acute and chronic inflammatory diseases. This study aims to investigate whether kaempferol affects systemic inflammation and oxidative stress in the heart, lung, and liver after hemorrhagic shock in mice.MethodsMale C57/BL6 mice underwent hemorrhagic shock (mean arterial pressure of 35 mmHg for 90 min) and were arbitrarily divided into Sham, hemorrhagic shock (HS), and Kae groups (n = 10 in each group). Mice in the Kae groups received a kaempferol (10-mg/kg body weight) injection 12 h prior to (Group Kae PT) or 90 min after (Group Kae T) the initiation of hemorrhagic shock. Plasma proinflammatory cytokines (TNF-α and IL-6), organ myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, and organ malondialdehyde (MDA) concentrations and heme oxygenase-1 (HO-1) expression levels were assessed by enzyme-linked immunosorbent assay (ELISA) or western blot assay.ResultsCompared with the HS group and the Kae T group, pretreatment with kaempferol significantly decreased proinflammatory cytokines TNF-α (P = 0.012 and 0.015, respectively) and IL-6 (P = 0.023 and 0.014, respectively) following hemorrhagic shock. Kae pretreatment reverted MPO, SOD, and MDA to basal levels in the heart, lung, and liver (Ps < 0.05), while the Kae T group showed no significant differences in these biomarkers compared with the HS group (Ps > 0.05). HO-1 expression was significantly increased in the Kae PT group compared with the other groups (P = 0.011 vs. HS group and P = 0.02 vs. Kae T group).ConclusionsPretreatment of hemorrhagic shock mice with kaempferol significantly decreased plasma levels of TNF-α and IL-6; reverted MPO, SOD, and MDA in the heart, lung, and liver; and increased expression of HO-1 in the same organs.

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Section: Discussionmentioning

confidence: 99%

Kaempferol pretreatment modulates systemic inflammation and oxidative stress following hemorrhagic shock in mice

Yang

He²,

Zhou

et al. 2015

Chin Med

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show abstract

“…These results are in accordance with previous studies. [35][36][37] Importantly, we performed a quantitative and specific measurement of O 2 −· production in the tested tissues, 38 since O 2 −· formation is the first step in a sequence of reactions leading to the oxidation of biological structures. In addition, we showed that H/S resulted in an increased concentration of endotoxin in the portal and systemic circulation, reflecting obviously the increased BT taking place after H/S.…”

Section: Discussionmentioning

confidence: 99%

Clonidine pre-treatment prevents hemorrhagic shock-induced endotoxemia and oxidative stress in the gut, liver, and lungs of the rat

et al. 2012

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“…SOD is extensively expressed, and MDA is able to counteract the effects of SOD and is cytotoxic (23). Wu et al (24) demonstrated that HS and resuscitation induce significant oxidation in the lungs of rats, while Panteli et al (25) reported that the oxidation state of the kidneys is uninfluenced after HS. In antioxidant case of HS, the treatment can ameliorate organ oxidative stress and achieve better efficacy.…”

Section: Groupmentioning

confidence: 99%

Effects of Alprostadil combined with Edaravone on inflammation, oxidative stress and Pulmonary function in patients with traumatic hemorrhagic shock

Luo¹,

Pan²,

Wang³

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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Quantification of superoxide radical production in 4 vital organs of rats subjected to hemorrhagic shock

Cited by 5 publications

References 20 publications

Kaempferol pretreatment modulates systemic inflammation and oxidative stress following hemorrhagic shock in mice

Kaempferol pretreatment modulates systemic inflammation and oxidative stress following hemorrhagic shock in mice

Clonidine pre-treatment prevents hemorrhagic shock-induced endotoxemia and oxidative stress in the gut, liver, and lungs of the rat

Effects of Alprostadil combined with Edaravone on inflammation, oxidative stress and Pulmonary function in patients with traumatic hemorrhagic shock

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