Quantification of Spironolactone by first and second order UV Derivative Spectrophotometry in bulk and tablet dosage form

Prajapati, Parixit; Chandarana, Chandni; Basuri, Tarashankar

doi:10.5958/2394-2797.2016.00024.1

Cited by 6 publications

(2 citation statements)

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“…The UV-spectrophotometric methods of determination of spironolactone only in methanolic (90,91) solutions were already developed. The method that utilises the derivative spectroscopy in methanolic solutions also exists (92).…”

Section: Methodsmentioning

confidence: 99%

UV spectra of aqueous or ethanolic solutions of twenty-seven active pharmaceutical ingredients

Nikolaychuk

2022

Scripta Scientifica Pharmaceutica

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INTRODUCTION: UV spectrophotometry is the simplest method of quantitative analysis of active pharmaceutical ingredients. It is widely used in developing active pharmaceutical ingredients, quantifying impurities, dissolution testing, and quantifying nucleic acids and proteins in pharmaceutical development. However, there is no convenient library of UV spectra of pharmaceutical ingredients in pure distilled water, and it is sometimes hard to find the necessary information in the literature.AIM: This study aims to create a dataset of UV spectra of 27 common pharmaceutical ingredients in aqueous or (for those insoluble in water) ethanolic solutions and to review the analytical methods of determination of these ingredients based on these spectral data that were already developed. MATERIALS AND METHODS:The aqueous (or ethanolic for those insoluble in water) solutions of 27 active pharmaceutical ingredients were prepared, and their UV spectra were recorded. RESULTS AND CONCLUSION:UV spectra of aqueous solutions of amlodipine besylate, articaine hydrochloride, bendazole hydrochloride, betaxolol hydrochloride, carbamazepine, citicoline sodium, chloropyramine, clopidogrel bisulfate, dexketoprofen trometamol, drotaverine hydrochloride, 2-ethyl-6-methyl-3-hydroxypyridine succinate, ketorolac trometamol, loperamide hydrochloride, menadione sodium bisulfite, metamizole sodium, metoclopramide hydrochloride, metoprolol tartrate, moxifloxacin hydrochloride, nicotinic acid (niacin), norepinephrine hydrochloride (noradrenaline hydrochloride), paracetamol (acetaminophen), and valacyclovir, and ethanolic solutions of carvedilol, haloperidol, indapamide, ketoprofen, and spironolactone are presented. The wavelengths of absorbance maxima for each compound are given. The methods of UV-spectrophotometric determination of these active pharmaceutical ingredients available in literature are reviewed.

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Section: Methodsmentioning

confidence: 99%

UV spectra of aqueous or ethanolic solutions of twenty-seven active pharmaceutical ingredients

Nikolaychuk

2022

Scripta Scientifica Pharmaceutica

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“…Several analytical methods for determination of both drugs were revealed through the literature survey. FR has been determined by various methods such as spectrophotometry (Gölcü, ; Semaan & Cavalheiro, ; Semaan, de Sousa, & Cavalheiro, ; Tescarollo Dias, Martins, & de Oliveira Neto, ), thin‐layer chromatography (TLC) (Mikkelsen & Andreasen, ), spectrofluorimetry (El‐Wasseef, El‐Sherbiny, Abu‐El‐Enein, & El‐Ashry, ; Peralta, Fernández, & Masi, ) and high‐performance liquid chromatography (HPLC) (Andreasen, Christensen, Jakobsen, & Mogensen, ; Guzmán, Agüı́, Pedrero, Yáñez‐Sedeño, & Pingarrón, ; Huclová et al, ; Semaan, dos Santos Neto, Lanças, & Gomes Cavalheiro, ), while, SP was determined by spectrophotometry (ERK, ; Prajapati, Chandarana, & Modi, ), chemometry/UV (Dinç & Üstündağ, ), TLC (Hegazy, Metwaly, Abdelkawy, & Abdelwahab, ) and HPLC (Hegazy et al, ). A binary mixture of both drugs was determined by spectrophotometric (Millership, Parker, & Donnelly, ) and HPLC methods (Ram, Dave, & Joshi, ).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma

Naguib

Abdelaleem

Emam

et al. 2018

Biomedical Chromatography

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Two selective and accurate chromatographic methods are presented for simultaneous quantitation of spironolactone (SP) and furosemide (FR) and canrenone (CN), the main degradation product and the main active metabolite of SP. Method A was HPTLC, where separation was completed on silica gel HPTLC F plates using ethyl acetate-triethylamine-acetic acid (9:0.7:0.5, by volume) as a developing system and UV detection at 254 nm. Method B was a green isocratic RP-HPLC utilizing a C (4.6 × 100 mm) column, the mobile phase consisting of ethanol-deionized water (45: 55, v/v) and UV estimation at 254 nm. Adjustment of flow rate at 1 mL/min and pH at 3.5 with glacial acetic acid was done. Regarding the greenness profile, the proposed RP-HPLC method is greener than the reported one. ICH guidelines were followed to validate the developed methods. Successful applications of the developed methods were revealed by simultaneous determination of FR, SP and CN in pure forms and plasma samples in the ranges of 0.2-2, 0.05-2.6 and 0.05-2 μg/band for method A and 5-60, 2-60 and 2-60 μg/mL for method B for FR, SP and CN, respectively.

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Estimation of Two Diuretics Using Fluorescent Nitrogen Doped Carbon Quantum Dots: Application to Spiked Human Plasma and Tablets

et al. 2023

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Highly fluorescent nitrogen doped carbon quantum dots (N-CQDs) were prepared by a single-step method based on microwave heating of cane sugar and urea. The produced N-CQDs were applied as nano-sensors for the spectrofluorimetric determination of eplerenone and spironolactone. A strong emission band at 376 nm was obtained after excitation at 216 nm due to the produced N-CQDs. The native fluorescence of N-CQDs was obviously quenched upon adding increased concentrations of each drug. A strong correlation was found between the fluorescence quenching of N-CQDs and the concentration of each drug. The method was found to be linear over the range of 0.5 to 5.0 μg/mL for eplerenone and 0.5 to 6.0 μg/mL for spironolactone with LOQ of 0.383 μg/mL and 0.262 μg/mL. The developed method was further extended for determination of both drugs in their pharmaceutical tablets and spiked human plasma. The results obtained were statistically compared with those of reported methods. The mechanism of fluorescence quenching of N-CQDs by the two drugs was discussed.

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Quantification of Spironolactone by first and second order UV Derivative Spectrophotometry in bulk and tablet dosage form

Cited by 6 publications

References 0 publications

UV spectra of aqueous or ethanolic solutions of twenty-seven active pharmaceutical ingredients

UV spectra of aqueous or ethanolic solutions of twenty-seven active pharmaceutical ingredients

Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma

Estimation of Two Diuretics Using Fluorescent Nitrogen Doped Carbon Quantum Dots: Application to Spiked Human Plasma and Tablets

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