Quantification of renal low-molecular-weight protein handling in the intact rat

Evaluation of a 99mTc-Labeled AnnexinA5 Variant for Non-invasive SPECT Imaging of Cell Death in Liver, Spleen and Prostate

“…This is in line with the biodistribution of other annexin variants (13)(14)(15) as well as with the distribution of other low molecular weight proteins, such as lysozyme, aprotinin and cytochrome c (16). Uptake into the renal cortex is thus considered to be apoptosis-independent and is likely to be mediated via initial glomerular filtration and subsequent re-uptake through high capacity / low specificity transport, for instance via Megalin receptors present in the luminal membrane of proximal tubular epithelial cells (17).…”

Pharmacokinetics and whole body distribution of elastase derived angiostatin(k1-3) in rats

“…15 Pharmacokinetic analysis of angiostatin (k1- 3) In vivo experiments. Animals were anaesthetised with Hypnorm/ Valium.…”

Renal-Selective Delivery and Angiotensin-Converting Enzyme Inhibition by Subcutaneously Administered Captopril-Lysozyme

“…As described previously, lysozyme was labeled with radioactive iodine via TC, a label that is retained intracellularly after lysosomal degradation of the protein (Haas et al, 1993). Briefly, the synthesis of TC was performed by reductive amination of cellobiose with tyramine (Pittman et al, 1983).…”

“…Gamma camera imaging was conducted under isoflurane anesthesia (2% isoflurane in 2:1 O 2 /N 2 O, 1 l/min) on a low-energy collimator at 0, 3, 6, 12, and 24 h following injections, according to a previously described protocol by Haas et al (1993). Body temperature was monitored and maintained at 37°C with a heat pad and a lamp.…”