2009
DOI: 10.1007/s00249-009-0532-z
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Abstract: Membrane proteins exhibit different affinities for different lipid species, and protein–lipid selectivity regulates the membrane composition in close proximity to the protein, playing an important role in the formation of nanoscale membrane heterogeneities. The sensitivity of Förster resonance energy transfer (FRET) for distances of 10 Å up to 100 Å is particularly useful to retrieve information on the relative distribution of proteins and lipids in the range over which protein–lipid selectivity is expected to… Show more

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Cited by 38 publications
(28 citation statements)
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References 69 publications
(28 reference statements)
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“…However, this kind of formalisms has been characterized by either severe simplifying approximations (Gutierrez-Merino, 1981; Brown et al, 2007a,b) or relying to some extent to numerical results (Towles et al, 2007; see Loura et al, 2010a for a detailed discussion), precluding their widespread use.…”
Section: Numerical and Simplified Analytical Treatments Of Fret In Nomentioning
confidence: 99%
“…In this case FRET (or quenching) is measured from a peptide-attached fluorophore to a labeled lipid [162]. Preferential interaction with that particular lipid will be demonstrated by a quenching efficiency higher than that expected for a random distribution of acceptors in the plane of the membrane.…”
Section: Lateral Mobility and Domain Formationmentioning
confidence: 99%
“…Variations in the separation and spatial arrangement of chromophores lead to alterations in FRET, that can be monitored under steady-state and/or time-resolved conditions. On a very basic level, FRET may be employed as a phenomenological indicator of molecular proximity, and a large body of work has been reported describing such qualitative applications [30]. However, to take full advantage of the technique's potential, models that describe the dependence of FRET observables on the structural properties of the system under study are required.…”
Section: The Biological Importance Of Electrostatic Lipid-protein Intmentioning
confidence: 99%
“…www.intechopen.com R 0 is an essential parameter for the quantitative description of FRET kinetics in membrane systems (Loura et al, 2010b(Loura et al, , 2010c as well as in the classic use of FRET as a spectroscopic ruler (Stryer, 1978). In the preceding equation, whereas Q D and J may be obtained from straightforward calculations from spectral data, there is no experimental technique suited to a definite measurement of  2 (though it was shown by Dale and co-workers (1979) that intervals containing its average value < 2 > can be inferred from adequate fluorescence anisotropy measurents).…”
Section: Nbd Probesmentioning
confidence: 99%
“…The results obtained with the non‐amyloidogenic proteins were intriguing, and prompted us to study the structure of these lipid‐protein supramolecular complexes, which are typically formed when using both a low ionic strength buffer and a high protein/lipid ratio in the experiments. Our team has developed state‐of‐the‐art methods of analysis for time‐resolved Förster resonance energy transfer (FRET) measurements, which have been applied to diverse problems in membrane biophysics such as lateral membrane domains, lipid/protein selectivity and peptide‐induced morphological alterations (reviewed in Loura et al. 2010a,b; Loura and Prieto 2009, respectively).…”
mentioning
confidence: 99%