Quantification of plasma lipoproteins by proton nuclear magnetic resonance spectroscopy

Abstract

A new analytical procedure for quantifying plasma lipoproteins by proton nuclear magnetic resonance (NMR) spectroscopy has been developed that potentially offers significant advantages over existing clinical methods used for assessing risk of coronary heart disease. Analysis of a single spectrum of a nonfasting plasma sample, acquired simply and rapidly at moderate magnetic field strength (250 MHz), yields a complete profile of lipoprotein concentrations: chylomicrons and very-low-, low-, and high-density lipoproteins. The method is based on curve-fitting (spectral deconvolution) of the plasma methyl lipid resonance envelope, the amplitude and shape of which depend directly on the amplitudes of the superimposed methyl resonances of the lipoprotein components. A linear least-squares curve-fitting algorithm was developed to efficiently extract the signal amplitudes (concentrations) of the lipoproteins from the plasma spectrum. These signal amplitudes correlate well with lipoprotein concentrations determined by triglyceride and cholesterol measurements.

A comparative evaluation of the metabolic profiles of normal and inflammatory knee‐joint synovial fluids by high resolution proton NMR spectroscopy

“…3). Indeed, LDL-and, to a much lesser extent, HDL-associated lipids make substantially greater contributions to the line-shape and intensity of the composite SF lipoprotein fatty acid acyl chain terminal-CH, and bulk (-CH,-), resonances at higher temperatures than they do at lower ones, a phenomenon attributable to the core cholesterol esters of these lipoproteins undergoing an order-disorder phase transition throughout this temperature range [8]. The corresponding signals of the triacylglycerol-rich chylomicron and VLDL particles do not undergo this phase transition, consistent with their core lipids being in a liquid-like state at temperatures 2 20°C.…”

Semi‐physiological model of postprandial triglyceride response in lean, obese and very obese individuals after a high‐fat meal

“…Lipoprotein subclasses were determined by nuclear magnetic resonance signals, broadcast by lipoprotein subclass particles of different sizes. 5 The measured amplitudes of these signals indicated the subclass concentrations. The VLDL subclasses and chylomicrons are reported as milligrams per decilitre of TGs.…”

Lipid assessment, metabolic syndrome and coronary heart disease risk

“…The inter-assay coefficients of variation of the apolipoprotein A-I and B measurements were 5% and 3% respectively. Proton NMR spectroscopy measurements were performed to measure VLDL, IDL, LDL and HDL particle concentration using a 400 megahertz proton NMR analyser at Liposcience, Inc. (Raleigh, NC, USA) as previously described [22]. Lipoprotein information was obtained with the NMR LipoProfile-2 analysis procedure.…”