Quantification of plaque neovascularization using contrast ultrasound: a histologic validation

Moguillansky, Diego; Leng, Xiaoping; Carson, Andrew R.; Lavery, Lawrence A.; Schwartz, Abigail; Chen, Xucai; Villanueva, Flordeliza S.

doi:10.1093/eurheartj/ehq197

Cited by 42 publications

(28 citation statements)

References 41 publications

(31 reference statements)

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“…Highly echogenic microbubbles passing through the vasa vasorum are readily visualized at contrastenhanced US ( 33,34 ). Quantitative and qualitative assessments of US enhancement have been shown to correlate with histologically quantifi ed microvessel density (35)(36)(37). Thus, contrast-enhanced US might be capable of showing therapeutic changes in the vasa vasorum in a manner similar to the way DCE MR imaging shows them .…”

Section: Effect On Adventitial Vasa Vasorummentioning

confidence: 99%

Carotid Artery Atherosclerosis: Effect of Intensive Lipid Therapy on the Vasa Vasorum—Evaluation by Using Dynamic Contrast-enhanced MR Imaging

Dong

Kerwin²,

Chen³

et al. 2011

Radiology

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Purpose:To investigate whether short-term, intensive lipid therapy leads to changes in microvascular characteristics, as measured by using dynamic contrast material-enhanced (DCE) magnetic resonance (MR) imaging. Materials and Methods:Institutional review board approval and informed consent were obtained for this HIPAA-compatible study. Subjects with established coronary artery disease or carotid artery stenosis of 15% or greater determined by using ultrasonography and with levels of apolipoprotein B of 120 mg/dL (1.2 g/L) or greater were enrolled in an ongoing study (clinical trial NCT00715273). All received intensive lipid therapy to achieve targeted high-and low-density lipoprotein cholesterol levels and underwent serial serum monitoring including high-sensitivity C-reactive protein (HsCRP) level measurements. Carotid artery MR imaging examinations including morphologic and DCE MR images were obtained at baseline and 1 year after treatment. In subjects with advanced lesions ( . 2 mm thick), MR image analysis was performed, including measurement of lipid-rich necrotic core size and kinetic modeling of DCE MR images to assess changes in the transfer constant ( K trans ). The differences in K trans between baseline and 1-year follow-up were compared by using the Wilcoxon signed rank test, and associations were assessed by using the Spearman rank correlation coeffi cient. Results:Twenty-eight subjects with interpretable DCE MR imaging results at both baseline and 1-year follow-up were included. After 1 year of treatment, a signifi cant reduction was found in mean K trans (0.085 min 2 1 6 0.037 [standard deviation] to 0.067 min 2 1 6 0.028, P = .02). Reduction in K trans was not signifi cantly correlated with observed reductions in lipid-rich necrotic core size or reductions in HsCRP level. Conclusion:These fi ndings suggest that DCE MR imaging may be a useful imaging method for the assessment of the therapeutic response of the vasa vasorum in patients with atherosclerotic plaque.Clinical trial registration no. NCT00715273.q RSNA, 2011

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Section: Effect On Adventitial Vasa Vasorummentioning

confidence: 99%

Carotid Artery Atherosclerosis: Effect of Intensive Lipid Therapy on the Vasa Vasorum—Evaluation by Using Dynamic Contrast-enhanced MR Imaging

Dong

Kerwin²,

Chen³

et al. 2011

Radiology

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“…At weeks 10 and 16, examination and analyses of CEUS data were undertaken as described elsewhere [9]. Briefly, all rabbits were anesthetized as described above.…”

Section: Ceus Imaging and Analysesmentioning

confidence: 99%

“…Furthermore, some studies have validated that the video intensity of CEUS peaks can be used to quantify the density of adventitial VV using histology [9]. CEUS could be used as a means to assess the influence of risk factors upon VV proliferation.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide-Induced Proliferation of the Vasa Vasorum in a Rabbit Model of Atherosclerosis as Evaluated by Contrast-Enhanced Ultrasound Imaging and Histology

Tian

Han

et al. 2012

Inflammation

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Whether lipopolysaccharide (LPS) can promote vasa vasorum (VV) proliferation for atherosclerosis in vivo is unclear. Eighteen rabbits with atherosclerosis were randomly assigned into one of three groups of six. Group A received biweekly injections of 10 mL saline after 2 weeks of balloon injury. Groups B and C received biweekly intravenous injections of 3.0 μg LPS in 10 mL saline at weeks 10 and 4, respectively, until study termination. LPS significantly increased the levels of triglycerides and C-reactive protein and decreased the level of high-density lipoprotein cholesterol. Group C had significant larger plaques and more macrophages than group A (p = 0.01 and p < 0.001, respectively). Contrast enhancement ultrasound imaging and histological detection demonstrated that plaques in group C had a significantly higher VV density than that in group A (p = 0.009 and p = 0.002, respectively). In summary, VV proliferation for plaque destabilization can be accelerated by LPS-induced systemic inflammation and changes in lipid profiles.

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“…Molecular and experimental imaging. Serial ultrasonography using microbubbles was employed to successfully track changes in plaque neovascularization in experimental atherosclerosis (60). Investigations using positron emission tomography demonstrated anti-inflammatory effects of pioglitazone on atherosclerotic plaques both in animal models (61) and in humans (62).…”

Section: Early Detection and Risk Stratificationmentioning

confidence: 99%

The Year in Atherothrombosis

Sanz

Moreno

Fuster

2012

Journal of the American College of Cardiology

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Quantification of plaque neovascularization using contrast ultrasound: a histologic validation

Abstract: We have histologically validated that CUS quantifies the development of adventitial vasa vasorum associated with atherosclerosis progression. This imaging technique has the potential for characterizing prognostically significant plaque features.

Cited by 42 publications

References 41 publications

Carotid Artery Atherosclerosis: Effect of Intensive Lipid Therapy on the Vasa Vasorum—Evaluation by Using Dynamic Contrast-enhanced MR Imaging

Carotid Artery Atherosclerosis: Effect of Intensive Lipid Therapy on the Vasa Vasorum—Evaluation by Using Dynamic Contrast-enhanced MR Imaging

Lipopolysaccharide-Induced Proliferation of the Vasa Vasorum in a Rabbit Model of Atherosclerosis as Evaluated by Contrast-Enhanced Ultrasound Imaging and Histology

The Year in Atherothrombosis

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