Quantification of Phenolic Constituents and Antioxidant Activity of Pterodon emarginatus Vogel Seeds

Abstract

Abstract:In the present study the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents and the antioxidant activity of Pterodon emarginatus seeds were investigated in several samples prepared with different extraction procedures: essential oil (EO) using a Clevenger-type apparatus; hexanic (HF), ethyl acetate (EAF), buthanolic (BF) and methanolic (MF) fractions using Soxhlet extraction, and extracts (1 g/extract) obtained from different methods: reflux 80°C/30 min, ultrasound/30 min, static maceration/48 h and heating plate 100°C/45 min. These extracts were prepared using water or ethanol/water at 30:70 v/v, 50:50 v/v or 70:30 v/v. Antioxidant activity [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH)] was tested only in the fractions obtained from Soxhlet extraction. The extract obtained from reflux using ethanol/water (70:30, v/v) showed the highest phenolic constituents level. The EAF, BF and MF showed DPPH scavenging activities with IC 50 =163.22, 18.89 and 10.15 μg/ml, respectively.

Anti-inflammatory, free radical-scavenging, and metal-chelating activities ofMalva parviflora

“…Medicinal plants are considered to be an important source of therapeutic compounds and the therapeutic benefit of many medicinal plants is often attributed to their anti-inflammatory and antioxidant properties (Rice-Evans, 2004;Shale et al, 2005;Zhang et al, 2009). The preservative effect of many plant species and herbs suggests the presence of antioxidative and antimicrobial constituents such as flavonoids, phenolic acids, and phenolic diterpenes (Shahidi & Wanasundara, 1992;Hammer et al, 1999;Dutra et al, 2008). Malva parviflora L. (Malvaceae) is widely distributed throughout Africa.…”

Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Five Selected Herbs

“…In recent years, the measurement and utilization of plant essential oils has become increasingly important in scientific research and industrial applications, including pharmaceutical, nutritional, and cosmetic uses, 1,2) primarily due to the oils' various potent biological activities, including antimicrobial, 3) antioxidant, 4) and anti-inflammatory 5) activities. The infection process frequently induces inflammation, and free radicals are released from the phagocytes during the inflammatory process.…”

Intoxicação espontânea por Pterodon emarginatus (Fabaceae) em bovinos no estado de Goiás

“…Embora numerosos trabalhos ϐitoquímicos tenham identiϐicados várias moléculas nas sementes de P. emarginatus, incluindo diterpenóides, isoϐlavonas e alcalóides (Dutra et al 2008), em apenas uma investigação recente foram detectados os compostos químicos presentes nas folhas da planta. Essa análise identiϐicou nove hidrocarbonetos sesquiterpênicos, sendo os principais o γ-muuroleno e o biciclogermacreno, e os esteróides estigmasterol e β-sitosterol (Santos et al 2010).…”