Quantification of phenolic compounds in ripe and unripe bitter melons (Momordica charantia) and evaluation of the distribution of phenolic compounds in different parts of the fruit by UPLC–MS/MS

Lopes, Ana Paula; Galuch, Marília B.; Petenuci, Maria Eugênia; Oliveira, Jean Halison de; Canesin, Edmilson Antônio; Schneider, Vanessa Vivian Almeida; Visentainer, Jesuí Vergílio

doi:10.1007/s11696-020-01094-5

Cited by 26 publications

(10 citation statements)

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“…For example, Horax et al (2010) reported that bitter melon of immature stage contained higher polyphenols than mature and ripe bitter melons, and seeds showed higher polyphenols than pericarp. The pulp of the ripe fruit and the whole unripe fruit showed the higher amounts of total phenolic compound compared to ripe peel and seed, and whole unripe fruit contained the most type of phenolic compounds (Lopes et al, 2020). The phenolic compounds contained in bitter melon showed the highest extraction efficiency when extracted using aqueous ethanol as a solvent (Lopes et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Optimization of ultrasound-assisted extraction of phenolic compounds from bitter melon (Momordica charantia) using response surface methodology

Lee

Yoon

2021

CyTA - Journal of Food

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Bitter melon is rich in phenolic compounds and has significant potential for commercial use as a functional food material. This study aims to optimize ultrasound-assisted extraction (UAE) conditions for extracting phenolic compounds from bitter melons using a response surface methodology. A central composite design was used to investigate the effects of three independent variables (ethanol concentration, ultrasonic power, and extraction time) on the responses, extraction yield, total polyphenol content (TPC), and antioxidant activity. The optimal UAE conditions were determined to be 59% and 277 W for 14 min, and the corresponding predicted response values for yield, TPC, and antioxidant activity were 33.42%, 18.73 mg GAE/g, and 66.93%, respectively. UAE showed higher extraction yield, TPC, phenolic content, and antioxidant activity than conventional ethanol extraction. These results suggest that bitter melon phenolic compounds obtained by the UAE could be used as natural antioxidant agent or functional ingredient enhancers in suitable food products.Optimización de la extracción asistida por ultrasonidos de compuestos fenólicos del melón amargo (Momordica charantia) mediante la metodología de superficie de respuesta RESUMENEl melón amargo es rico en compuestos fenólicos y posee un importante potencial para ser usado comercialmente como material alimentario funcional. Con el objetivo de obtener compuestos fenólicos del melón amargo utilizando la metodología de superficie de respuesta, el presente estudio se propuso optimizar las condiciones de extracción asistida por ultrasonidos (UAE). Para ello se utilizó un diseño central compuesto a fin de investigar los efectos de tres variables independientes (concentración de etanol, potencia ultrasónica y tiempo de extracción) en las respuestas, rendimiento de extracción, contenido total de polifenoles (TPC) y actividad antioxidante. Se determinó que las condiciones óptimas de UAE son 59% y 277 W durante 14 minutos, mientras que los correspondientes valores de respuesta previstos para el rendimiento, el TCP y la actividad antioxidante son 33.42%, 18.73 mg GAE/g y 66.93%, respectivamente. Asimismo, se comprobó que la UAE posee mayor rendimiento de extracción, TPC, contenido fenólico y actividad antioxidante que la extracción convencional con etanol. Estos resultados dan cuenta de que los compuestos fenólicos del melón amargo obtenidos mediante UAE pueden utilizarse como agentes antioxidantes naturales o para potenciar ingredientes funcionales en productos alimentarios adecuados.

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Section: Introductionmentioning

confidence: 99%

Optimization of ultrasound-assisted extraction of phenolic compounds from bitter melon (Momordica charantia) using response surface methodology

Lee

Yoon

2021

CyTA - Journal of Food

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“…Vahl. (Acanthaceae) are among the tropical plants used for the treatment of various ailments [4][5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…Momordica charantia L. (Cucurbitaceae) is used in the Amazon, parts of Asia and Africa for its numerous benefits in the treatment of skin infections. The fruits and leaves have been observed to contain saponin-like substances and phenolic compounds [5]. The plant has also been shown to contain anti-tumor properties [6].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country against Bacterial Pathogens from Post-Operative Wound Infections

et al. 2021

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Background: Globally, the application of medicinal plants in the management of acute and chronic wounds can be considered a common occurrence in most traditional medicine practices. In view of this, many plants in the tropical and subtropical regions have been screened for their wound-healing activities. Consequently, plants having antimicrobial activity against multidrug-resistant (MD-R) pathogens can be considered great assets. Therefore, this study evaluated ethanolic and aqueous extracts of five medicinal plants (Psidium guajava, Myrianthus arboreus, Alchornea cordifolia, Momordica charantia, and Justicia flava) for their antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds; Methods: This involved the aqueous and ethanolic extraction of the selected medicinal plants. Preliminary phytochemical constituents of the plants were examined. The agar well diffusion method was then used to determine the antibacterial activity of the leaves against reference strains (Escherichia coli ATCC 25922, Salmonella typhi ATCC 19430, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and a Methicillin-Resistant Staphylococcus aureus strain) as well as the MDR clinical isolates (E. coli, P. aeruginosa, S. aureus and CoNS) from the wounds; Results: The preliminary phytochemical analysis of the leaves showed the presence of saponins, phenolics, and reducing sugars in almost all the plants tested. All plant extracts were observed to have some antimicrobial activity against at least one reference strain. For the clinical MDR isolates, A. cordifolia from this study showed highest inhibition to growth of all bacteria used. Activity of J. flava against S. aureus was highest as compared to that of E.coli and P. aeruginosa. Similar observation was made for M. arboreus, P. guajava and M. charantia where the highest activity was observed against S. aureus; Conclusion: This study has mainly shown that P. guajava, M. arboreus, A. cordifolia, M. charantia, and J. flava exhibits antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds. Also, these plants has bioactive phytochemical compounds with potential medicinal values for the treatment of numerous infections. Therefore, these plants may be helpful in the management of acute and chronic wounds, especially in traditional medicine practices.

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“…The membranes of the seeds of ripe fruits are a good source of lycopene [7][8][9]. Leaves and unripe fruits of the plant, rich in vitamins and minerals, are consumed as vegetables Since the taste of ripe fruits is bitter, the unripe form is preferred when consumed as vegetables [10][11]. Unripe fruits, ripe fruits or preparations made from whole plants and teas prepared from leaves are used for treatment or health protection.…”

Section: Introductionmentioning

confidence: 99%

Comparison of antioxidant capacities and antioxidant components of commercial bitter melon (Momordicacharantia L.) products

Akyüz

TÜrkoĞlu²,

Başkan

et al. 2020

Turk J Chem

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In this study, the total phenolic contents and total antioxidant capacities of some commercial bitter melon products (powder, packaged powder, capsule, paste in olive oil), and of unripe and ripe fruits were determined by spectrophotometric and chromatographic methods. The total antioxidant capacities of unripe and ripe bitter melon samples, determined by CUPRAC (Cupric Reducing Antioxidant Capacity Assay) and ABTS (2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid)/HRP (Horseradish peroxidase) methods, were 42.5 and 36.3 µmol TRE (trolox equivalent) g-1 , 8.7 and 7.0 µmol TRE g-1 , respectively. The TAC (total antioxidant capacity) order of studied samples using the same two methods were determined as follows; capsule (CUPRAC value, 140.8; ABTS/HRP value, 143.6 µmol TRE g-1) > packaged powder (129.6; 126.1) 2 > powder (52.3; 64.3) > unripe fruit (42.5; 36.3) > paste in olive oil (17.6; 14.4) > ripe 25 fruit (8.7; 7.0). Their order of phenolic content was found as follows; unripe fruit (193.2 26 µmol GAE (gallic acid equivalent) g-1) > capsule (162.0) > packaged powder (160.6) > 27 powder (83.6) > paste in olive oil (38.3) > ripe fruit (14.6).

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Quantification of phenolic compounds in ripe and unripe bitter melons (Momordica charantia) and evaluation of the distribution of phenolic compounds in different parts of the fruit by UPLC–MS/MS

Cited by 26 publications

References 50 publications

Optimization of ultrasound-assisted extraction of phenolic compounds from bitter melon (Momordica charantia) using response surface methodology

Optimization of ultrasound-assisted extraction of phenolic compounds from bitter melon (Momordica charantia) using response surface methodology

Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country against Bacterial Pathogens from Post-Operative Wound Infections

Comparison of antioxidant capacities and antioxidant components of commercial bitter melon (Momordicacharantia L.) products

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