Quantification of Phenolic Component by LC‐HESI‐MS/MS and Evaluation of Antioxidant Activities of <i>Crocus Ancyrensis</i> (Ankara Çiğdemi) Extracts Obtained with Different Solvents

Kayır, Ömer; Doğan, Hacer; Alver, Erol; Bilici, İbrahim

doi:10.1002/cbdv.202201186

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…ODS Hypersil 4.6 * 250 mm column was used. The MS/MS analysis conditions are as follows: capillary temperature: 300 °C, vaporizer temperature: 350 °C, sheat gas pressure: 30 Arb, Aux gas pressure: 13 Arb, positive polarity: 2500 V, negative polarity: 2500 V, discharge current: 4 µA 14 , 15 . Quantification was evaluated according to MS/MS.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical fingerprint and biological activity of raw and heat-treated Ornithogalum umbellatum

Akgeyik,

Yalçın,

Çavuşoğlu

2023

Sci Rep

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The plants that we use as food in our daily diet and as risk preventers against many diseases have many biological and pharmacological activities. The heat treatments applied during the cooking of the plants cause changes in the phytochemical content and bioactivity. In this study, the phytochemical fingerprint and biological activities of raw and heat-treated extracts of Ornithogalum umbellatum L., which is widely consumed in the Black Sea region, were investigated. The bulb and leaf parts of the plant consumed as food were dried in an oven at 35 °C and then ground into powder. For heat treatment, the plant was boiled at 100 °C for 20 min. Differences in phytochemical contents of raw and heat-treated extracts were determined by ICP-MS, LC–MS/MS, and FTIR analysis. Biological activity was investigated with antiradical, antimicrobial, antimutagenic and antiproliferative activity tests. In this way, the effect of heat treatment on both the phytochemical content and biological activity of the O. umbellatum extract was determined. Gallic acid, procateuic acid and caffeic acid were found as the main compounds in the O. umbellatum extract, while the presence of procateuic aldehyde, vanillin and kaempferol in minor proportions was determined. There was a significant decrease in phenolic compound levels after heat treatment and gallic acid content decreased by 92.6%, procateuic acid content by 90% and caffeic acid content by 84.8%. Significant differences were detected in macro and micro element levels after heat treatment in ICP-MS results. While Cd, Ba and Zn levels of the raw extract increased; Na, Mg, K, Fe, U, Co levels decreased significantly. In FTIR spectrum, shifts and disappearances were observed in some of the vibrations and the emergence of new vibrations was also determined after heat treatment. Raw extract exhibited strong scavenging activity against H2O2 and DPPH and had a broad spectrum antimicrobial property. As a result of heat application, regressions were detected in antiradicalic, antibacterial and antifungal activities. Antimutagenic and antiproliferative activities were determined by the Allium test and a significant decrease in both activities and loss of activity against some chromosomal abnormalities were determined after heat treatment. While the antiproliferative activity of the raw extract was 20%, the activity of the heat-treated extract decreased to 7.6%. The raw extract showed the strongest antimutagenic effect with 69.8% against the unequal distribution of chromatin. Similarly, the antimutagenic activity of the extract, which reduced the bridges by 56.1%, decreased to 0.74% after heat treatment and almost lost its antimutagenic activity. The biological activities of raw O. umbellatum are closely related to the major compounds it contains, and the decrease in the levels of these compounds with the effect of heat was reflected in the activity. Studies investigating the phytochemical contents of plants are very important and the studies investigating biological activities related to phytochemical content are more remarkable. In this study, the phytochemical fingerprint of O. umbellatum was determined, its biological activities were related to the compounds it contained, and the biological activity was found to be heat sensitive.

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Section: Methodsmentioning

confidence: 99%

Phytochemical fingerprint and biological activity of raw and heat-treated Ornithogalum umbellatum

Akgeyik,

Yalçın,

Çavuşoğlu

2023

Sci Rep

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“…The column oven was set to 30 °C, the solvent flow rate was 0.7 mL/min and the analysis lasted for 34 min. The following are the criteria for the MS/MS analysis: Temperatures of the capillary are 300 °C, the vaporizer is 350 °C, the sheat gas pressure is 30 Arb, the aux gas pressure is 13 Arb, the positive and negative polarities are 2500 V and 4 µA, respectively 31 , 32 .…”

Section: Methodsmentioning

confidence: 99%

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Üstündağ,

Macar,

Kalefetoğlu Macar

et al. 2023

Sci Rep

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Although the antioxidant properties of Melissa officinalis extract (Mox) are widely known, little work has focused on its protective capacity against heavy metal stress. The primary objective of this study was to determine the potential of Mox to mitigate manganese (II) chloride (MnCI2)-induced cyto-genotoxicity using the Allium and comet assays. Physiological, genotoxic, biochemical and anatomical parameters as well as the phenolic composition of Mox were examined in Allium cepa (L.). Application of 1000 µM MnCl2 reduced the rooting percentage, root elongation, weight gain, mitotic index and levels of chlorophyll a and chlorophyll b pigments compared to the control group. However, it increased micronuclei formation, chromosomal abnormality frequencies, tail DNA percentage, proline amount, lipid peroxidation level and meristematic damage severity. The activities of superoxide dismutase and catalase also increased. Chromosomal aberrations induced by MnCl2 were fragment, sticky chromosome, vagrant chromosome, unequal distribution of chromatin and bridge. Application of 250 mg/L Mox and 500 mg/L Mox along with MnCl2 significantly alleviated adverse effects dose dependently. The antioxidant activity bestowed by the phenolic compounds in Mox assisted the organism to combat MnCl2 toxicity. Consequently, Mox exerted remarkable protection against MnCl2 toxicity and it needs to be investigated further as a potential therapeutic option.

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“…The mobile phase employed for this study was selected after a combination of several trials to determine the optimal ionization and separation of the molecules. The compounds listed in Table 1 were analyzed using LC MS/MS ( Kayir et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

The ameliorative effect of Primula vulgaris on cisplatin-induced nephrotoxicity in rats and quantification of its phenolic components using LC-ESI-MS/MS

Turan

Canbolat

Demir

et al. 2023

Saudi Pharmaceutical Journal

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Quantification of Phenolic Component by LC‐HESI‐MS/MS and Evaluation of Antioxidant Activities of Crocus Ancyrensis (Ankara Çiğdemi) Extracts Obtained with Different Solvents

Cited by 8 publications

References 24 publications

Phytochemical fingerprint and biological activity of raw and heat-treated Ornithogalum umbellatum

Phytochemical fingerprint and biological activity of raw and heat-treated Ornithogalum umbellatum

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

The ameliorative effect of Primula vulgaris on cisplatin-induced nephrotoxicity in rats and quantification of its phenolic components using LC-ESI-MS/MS

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