Quantification of pentane in exhaled breath, a potential biomarker of bowel disease, using selected ion flow tube mass spectrometry

Dryahina, Kseniya; Španěl, Patrik; Pospíšilová, Veronika; Sovová, Kristýna; Hrdlička, Luděk; Machková, Naděžda; Lukáš, Milan; Smith, David

doi:10.1002/rcm.6660

Cited by 65 publications

(66 citation statements)

References 54 publications

(133 reference statements)

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“…In eNose devices, the physical quantity of each individual sensor, such as electrical resistance or oscillation frequency, can be influenced by the entire spectrum of VOCs present in a gaseous mixture [3]. Confounding VOCs, originating from non-relevant or non-avoidable environmental, host-specific or sampling-specific sources, may predominantly interact with these nanosensors, instead of those VOCs of potential interest [29,30]. Contrariwise, whereas a single VOC alteration based on a given confounder may cause significant changes in GC-MS results, this could have negligible effect on the array of cross-reactive sensors in an eNose [15].…”

Section: Discussionmentioning

confidence: 99%

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Berkhout

Benninga

Stein

et al. 2016

Sensors

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Prior to implementation of volatile organic compound (VOC) analysis in clinical practice, substantial challenges, including methodological, biological and analytical difficulties are faced. The aim of this study was to evaluate the influence of several sampling conditions and environmental factors on fecal VOC profiles, analyzed by an electronic nose (eNose). Effects of fecal sample mass, water content, duration of storage at room temperature, fecal sample temperature, number of freeze–thaw cycles and effect of sampling method (rectal swabs vs. fecal samples) on VOC profiles were assessed by analysis of totally 725 fecal samples by means of an eNose (Cyranose320®). Furthermore, fecal VOC profiles of totally 1285 fecal samples from 71 infants born at three different hospitals were compared to assess the influence of center of origin on VOC outcome. We observed that all analyzed variables significantly influenced fecal VOC composition. It was feasible to capture a VOC profile using rectal swabs, although this differed significantly from fecal VOC profiles of similar subjects. In addition, 1285 fecal VOC-profiles could significantly be discriminated based on center of birth. In conclusion, standardization of methodology is necessary before fecal VOC analysis can live up to its potential as diagnostic tool in clinical practice.

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Section: Discussionmentioning

confidence: 99%

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Berkhout

Benninga

Stein

et al. 2016

Sensors

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show abstract

“…However, the use of m/z values for biomarker detection is unreliable, especially at higher m/z , as ions could represent any or many of a number of compounds. Recently, Dryahina and co-workers [145] reported pentane as potential biomarker of bowel disease, by analysing the breath of patients with Crohn’s disease and ulcerative colitis and of healthy volunteers, in a pilot study using SIFT-MS.…”

Section: Disease Diagnosismentioning

confidence: 99%

Breath Analysis in Disease Diagnosis: Methodological Considerations and Applications

2014

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Breath analysis is a promising field with great potential for non-invasive diagnosis of a number of disease states. Analysis of the concentrations of volatile organic compounds (VOCs) in breath with an acceptable accuracy are assessed by means of using analytical techniques with high sensitivity, accuracy, precision, low response time, and low detection limit, which are desirable characteristics for the detection of VOCs in human breath. “Breath fingerprinting”, indicative of a specific clinical status, relies on the use of multivariate statistics methods with powerful in-built algorithms. The need for standardisation of sample collection and analysis is the main issue concerning breath analysis, blocking the introduction of breath tests into clinical practice. This review describes recent scientific developments in basic research and clinical applications, namely issues concerning sampling and biochemistry, highlighting the diagnostic potential of breath analysis for disease diagnosis. Several considerations that need to be taken into account in breath analysis are documented here, including the growing need for metabolomics to deal with breath profiles.

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“…Differences in 7 VOCs were found in the headspace of vapor from urine samples in patients with gastro-esophageal cancer [160,161]. Pentane has been examined as a potential biomarker of bowel disease [162]. A careful analysis of changes in breath metabolites associated with advanced chronic kidney disease (CKD) has been undertaken and outlined in a review paper [163].…”

Section: Medicalmentioning

confidence: 99%

Direct Analysis Mass Spectrometry

McEwan

2015

Ion/Molecule Attachment Reactions: Mass Spectrometry

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Quantification of pentane in exhaled breath, a potential biomarker of bowel disease, using selected ion flow tube mass spectrometry

Abstract: SIFT-MS can be used to quantify pentane in human breath in real time avoiding sample storage. This method of analysis can ultimately form the basis of non-invasive screening of inflammatory processes, including inflammatory bowel disease.

Cited by 65 publications

References 54 publications

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Breath Analysis in Disease Diagnosis: Methodological Considerations and Applications

Direct Analysis Mass Spectrometry

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