Quantification of pathogenic marine vibrio using membrane filter technique

Hernández‐López, Jorge; Guzmán-Murillo, Ma.Antonia; Vargas‐Albores, Francisco

doi:10.1016/0167-7012(94)00042-6

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…An additional filtration unit was placed in each sampled area for bacterial counting, as a punctual contamination marker. The UFC count was recorded, as by Nobile (1967) and Hernández-López et al (1995), with some modifications. In brief, 22 mm diameter filters with 0.22 mm pores and petri plates with tryptic soy agar (TSA) containing 0.1% of tetrazolium violet were used and incubated for 24-48 h at 35 C. Colony forming units (CFU) were counted and the bacteria that were obtained were analyzed by colonial morphology, Gram staining and biochemical reactions.…”

Section: Bacterial Count In Sampling Areasmentioning

confidence: 99%

Detection of Sars-Cov-2 in the air of two hospitals in Hermosillo, Sonora, México, utilizing a low-cost environmental monitoring system

López¹,

Romo²,

Molina³

et al. 2021

International Journal of Infectious Diseases

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Highlights SARS-CoV-2 virus can be detected in PM air using 0.22 µm pore filters. The virus presence was observed only in the close areas from patients in hospitals. Sampling air during 3 h was sufficient time for virus detect. The system implemented for air sampling can be used for detection of SARS-CoV-2 in high-risk environments.

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Section: Bacterial Count In Sampling Areasmentioning

confidence: 99%

Detection of Sars-Cov-2 in the air of two hospitals in Hermosillo, Sonora, México, utilizing a low-cost environmental monitoring system

López¹,

Romo²,

Molina³

et al. 2021

International Journal of Infectious Diseases

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Section: Introductionmentioning

confidence: 99%

“…Different representatives of the Vibrio genus are found in most, if not all, marine fish hatcheries. Although most of these bacteria are relatively harmless at low levels, when allowed to proliferate within culture systems, they can cause disease and mortality of larval marine fish (Hernandez-Lopez, Guzman-Murillo & Vargas-Albores 1995;Vadstein et al 2004Vadstein et al , 2013. The presence and quantity of Vibrio colonies can serve as an indicator for other opportunistic bacteria, and while low numbers of bacteria are not beneficial by default, a large number of Vibrio colonies, is considered negative for performance of young marine fish larvae (Munro et al 1994;Verner-Jeffreys et al 2006;Reid, Treasurer, Adam & Birkbeck 2009;Attramadal, Tondel, Salvensen, Oie, Vadstein & Olsen 2012).…”

Section: Introductionmentioning

confidence: 99%

Methods of microbial control in marine fish larval rearing: clay-based turbidity and passive larval transfer

2015

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This study focused on methods to reduce bacterial loads in the larval culture tanks of California yellowtail (Seriola lalandi). We conducted two trials to evaluate methods to minimize bacterial loads in the larval rearing water. The first trial examined the use of bentonite clay as a turbidity agent to replace algae in a green water-type environment. This trial consisted of three treatments: (1) clay with continuous feeding (CCO), (2) clay with batch feedings (CBA) and (3) algae paste with batch feedings (ALG). The results showed that both clay treatments had significantly fewer Vibrio colonies in the water column (CBA -180 AE 78; CCO -377 AE 120 CFU mL À1 ) than the ALG treatment (5692 AE 2396 CFU mL À1 ) after 14 days of culture. Survival was significantly higher in the CCO treatment (14.1 AE 2.6%) than either the CBA (2.3 AE 0.5%) or ALG treatments (2.8 AE 1.5%). The second trial attempted to limit bacterial loading in the larval culture tank by passively transferring the larvae into an adjacent, clean tank at 1, 5 and 9 days post hatch during the first 2 weeks of culture. The results from this trial showed that after 12 days of culture, water in the transfertank had fewer Vibrio colonies (1025 AE 541 CFU mL À1 ) than the water in the control tanks (1962 AE 1415 CFU mL À1 ). Also, survival was significantly higher among larvae that were transferred (43.9 AE 13.5%) than in the control tanks (23.1 AE 6.3%).

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“…Considering that vibrios are widely distributed in seawater, and that some species can grow between 40" and 42"C, MI; procedures were performed using TCBS as culture medium (TCBS-MF) for vibrio quantification (Hernandez-Lopez et al 1994). Six samples were analysed by MFTC, MFFC, MF7h and TCBS-MF.…”

Section: Resultsmentioning

confidence: 99%

False-positive coliform readings using membrane filter techniques for seawater

Hernández‐López¹,

Vargas‐Albores²

1994

Lett Appl Microbiol

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Seawater samples that had been analysed by membrane filter techniques produced positive coliform readings that were found to be false. Apparently, the culture media and the incubation temperatures used in these methods permit the growth of vibrios which are abundant in the seawater. The vibrio colonies isolated by membrane filter were oxidase‐positive Gram‐negative short rods that grew on TCBS and required NaCl.

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Quantification of pathogenic marine vibrio using membrane filter technique

Cited by 10 publications

References 13 publications

Detection of Sars-Cov-2 in the air of two hospitals in Hermosillo, Sonora, México, utilizing a low-cost environmental monitoring system

Detection of Sars-Cov-2 in the air of two hospitals in Hermosillo, Sonora, México, utilizing a low-cost environmental monitoring system

Methods of microbial control in marine fish larval rearing: clay-based turbidity and passive larval transfer

False-positive coliform readings using membrane filter techniques for seawater

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