Quantification of Non-Transferrin-Bound Iron in the Presence of Unsaturated Transferrin

Gosriwatana, Itsaraet; Loréal, Olivier; Lu, Shuli; Brissot, Pierre; Porter, John B.; Hider, Robert C.

doi:10.1006/abio.1999.4216

Cited by 157 publications

(150 citation statements)

References 23 publications

Supporting

Mentioning

145

Contrasting

Order By: Relevance

“…In the iron overload scenarios like thalassemia; hemochromatosis and others iron gets a chance to exist in form which is not bond to Tf [2,[14][15][16][17][18]. Increased levels of NTBI in the above mentioned situations is easily explainable whereas it is difficult to give a convenient explanation for the raised levels of NTBI where Tf saturation is not high [18][19][20]. There is ample justification that why earlier studies of NTBI were focused in the diseases where the saturation of Tf was expected to high due to iron overload.…”

Section: Sources and Status Of Ntbi In Circulationmentioning

confidence: 99%

“…It has been observed that with the increase in the concentration of NTA there is also a parallel increase in the concentration of NTBI estimated [63]. In the method described by Gosriwatana et al [19,62,64,65] 80 mM NTA was suggested as compared to the other methods where lower concentrations of NTA was used. However Kolb et al [63] suggested that 4 mM NTA is the best suitable concentration for chelation of NTBI.…”

Section: Chelation Of Ntbi With Chelator Followed By Its Separation Amentioning

confidence: 99%

See 1 more Smart Citation

Non Transferrin Bound Iron: Nature, Manifestations and Analytical Approaches for Estimation

Patel

Ramavataram

2012

Ind J Clin Biochem

View full text Add to dashboard Cite

show abstract

Section: Sources and Status Of Ntbi In Circulationmentioning

confidence: 99%

Section: Chelation Of Ntbi With Chelator Followed By Its Separation Amentioning

confidence: 99%

Non Transferrin Bound Iron: Nature, Manifestations and Analytical Approaches for Estimation

Patel

Ramavataram

2012

Ind J Clin Biochem

View full text Add to dashboard Cite

show abstract

“…The problem can be minimized by selectively blocking vacant iron-binding sites on transferrin with cobalt(III) [12]. This cannot be achieved with simple cobalt(II) or cobalt(III) salts, but requires the use of tris -carbonatocobaltate(III) trihydrate, a reagent developed by He and coworkers [50].…”

Section: Use Of Iron(iii) Scavenging Moleculesmentioning

confidence: 99%

“…Analysis was extended to haemochromatosis patients and again NTBI was detected [10,11]. Surprisingly, NTBI was also detected in some patients with partially saturated transferrin [5,11], a finding recently confirmed using a HPLC-based assay [12][13][14]. NTBI is also present in patients receiving chemotherapy, a phenomena resulting from the temporary 'shutdown' of the bone marrow and the associated reduction of the demand of transferrin-bound iron [15][16][17][18].…”

Section: Introductionmentioning

confidence: 96%

Nature of nontransferrin‐bound iron

Hider

2002

Eur J Clin Investigation

128

View full text Add to dashboard Cite

Nontransferrin-bound iron is now recognized to exist by many workers. It is present in the serum of patients suffering from a wide range of disease states and may be induced by certain therapeutic treatments. The chemical nature of this iron pool is unknown but almost certainly it is a multicomponent pool including a considerable proportion of protein-bound iron.Methods are required to separate and quantify these different components. The biological properties of the individual isoforms need to be established; it is possible that some forms are relatively nontoxic, while others are highly toxic. This paper reviews what is known about the nature of nontransferrin-bound iron and describes the methods currently available to quantify this important serum component.

show abstract

“…These forms, referred collectively as non-TBI or Non-transferrin bound iron (NTBI), appear in plasma when Tf binding capacity for iron is exceeded due to massive iron overload, such as occurs in thalassemia major [3] and other congenital anemias [4], which require repeated blood transfusions, or bone marrow failure where iron utilization is inefficient [5]. NTBI may also occur in primary iron overload such as thalassemia intermedia or hemochromatosis even in the setting of < 100% Tf saturation [6][7][8], due apparently to formation of insoluble polynuclear ferrihydrate species [9] and complexes with modified albumin [10] that are inaccessible to Tf.…”

Section: Introductionmentioning

confidence: 99%

Non‐transferrin bound iron in Thalassemia: Differential detection of redox active forms in children and older patients

Breuer¹,

Ghoti

Shattat³

et al. 2011

American J Hematol

View full text Add to dashboard Cite

Non-transferrin bound iron (NTBI) is commonly detected in patients with systemic iron overload whose serum iron-binding capacity has been surpassed. It has been perceived as an indicator of iron overload, impending organ damage and a chelation target in poly-transfused thalassemia patients. However, NTBI is a heterogeneous entity comprising various iron complexes, including a significant redox-active and readily chelatable fraction, which we have designated as ''labile plasma iron'' (LPI). We found that LPI levels can be affected by plasma components such as citrate, uric acid, and albumin. However, the inclusion of a mild metal mobilizing agent in the LPI assay (designated here as ''eLPI''), at concentrations that do not affect transferrin-bound iron, largely overcomes such effects and provides a measure of the full NTBI content. We analyzed three distinct groups of poly-transfused, iron overloaded thalassemia patients: non-chelated children (3-13 yrs, Gaza, Palestine), chelated adolescents-young adults (13-28 yrs, Israel), and chelated adults (27-61 yrs, Israel) for LPI and eLPI. The eLPI levels in all three groups were roughly commensurate (r 2 5 0.61-0.75) with deferrioxamine-detectable NTBI, i.e., DCI. In older chelated patients, eLPI levels approximated those of LPI, but in poly-transfused unchelated children eLPI was notably higher than LPI, a difference attributed to plasma properties affected by labile iron due to lack of chelation, possibly reflecting age-dependent attrition of plasma components. We propose that the two formats of NTBI measurement presented here are complementary and used together could provide more comprehensive information on the forms of NTBI in patients and their response to chelation. Am. J. Hematol. 87:55-61, 2012. V

show abstract

Quantification of Non-Transferrin-Bound Iron in the Presence of Unsaturated Transferrin

Cited by 157 publications

References 23 publications

Non Transferrin Bound Iron: Nature, Manifestations and Analytical Approaches for Estimation

Non Transferrin Bound Iron: Nature, Manifestations and Analytical Approaches for Estimation

Nature of nontransferrin‐bound iron

Non‐transferrin bound iron in Thalassemia: Differential detection of redox active forms in children and older patients

Contact Info

Product

Resources

About