Quantification of nicotine, cotinine, trans-3′-hydroxycotinine and varenicline in human plasma by a sensitive and specific UPLC–tandem mass-spectrometry procedure for a clinical study on smoking cessation

Dobrinas, Maria; Choong, Eva; Noetzli, Muriel; Cornuz, Jacques; Ansermot, Nicolas; Eap, Chin B.

doi:10.1016/j.jchromb.2011.09.046

Cited by 38 publications

(48 citation statements)

References 43 publications

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“…Our proposed study, in comparison with the reported methods is highly efficient, more specific and highly sensitive for quantitative determination of VRC in both human plasma and dosage form compared with published methods (Obach et al, 2006;Dobrinas et al, 2011). Both methods (Obach et al, 2006;Dobrinas et al, 2011) were used as gradient elution.…”

Section: Methods Developmentmentioning

confidence: 93%

“…Both methods (Obach et al, 2006;Dobrinas et al, 2011) were used as gradient elution. In the first method (Obach et al, 2006), the column was washed and reequilibrated after each injection and the method was not fully validated.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Two LC-MS/MS methods have been published for the quantification of varenicline in human plasma, the first one was done by Obach et al, (2006) which has then been applied for the study of varenicline pharmacokinetics Faessel et al, 2007). The second method was developed by Dobrinas et al (2011) for determination of nicotine, cotinine, trans-3'-hydroxycotinine and varenicline, which was performed as a procedure for a clinical study on smoking cessation to confirm abstinence from smoking and detection of overdose.…”

Section: Introductionmentioning

confidence: 99%

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An isocratic liquid chromatography-electrospray ionization tandem mass spectrometric determination of varenicline in human plasma and dosage form

Kassem¹

2013

Afr. J. Pharm. Pharmacol.

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Section: Methods Developmentmentioning

confidence: 93%

Section: Methods Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An isocratic liquid chromatography-electrospray ionization tandem mass spectrometric determination of varenicline in human plasma and dosage form

Kassem¹

2013

Afr. J. Pharm. Pharmacol.

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“…Available clinical-trial data support its use as an effective and generally well-tolerated therapy for smoking cessation in healthy adult smokers [6]. High performance liquid chromatography (HPLC) is the predominant method used for varenicline analysis [7][8][9][10][11][12][13][14]. Other analytical techniques are used sporadically, including mainly spectroscopic techniques [10,15,16].…”

Section: Development and Validation Of The Hplc Methods For Vareniclinmentioning

confidence: 99%

“…Other analytical techniques are used sporadically, including mainly spectroscopic techniques [10,15,16]. Varenicline has been analyzed using UV-VIS spectrophotometry [15], fluorimetry [16], nuclear magnetic resonance spectroscopy (1H NMR and 13C NMR) [10], mass spectrometry (MS) [10,13,14] and Fourier-transform infrared spectroscopy (FTIR) [10]. Most frequently, papers refer to the quantification of varenicline in its pharmaceutical preparations [7][8][9][10][11][12]15,16].…”

Section: Development and Validation Of The Hplc Methods For Vareniclinmentioning

confidence: 99%

Development and validation of the HPLC method for varenicline determination in pharmaceutical preparation

Pietraś

Szymańska

Kondej

et al. 2017

Current Issues in Pharmacy and Medical Sciences

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A simple and accurate reverse phase HPLC method has been developed and validated for quantification of varenicline in bulk drug and pharmaceutical dosage forms. Herein, an isocratic LC analysis was carried out on a Chromolith Performance RP18e column with methanol-buffer solution pH 3.5 (a buffer solution containing sodium benzoate (0.5 mmol/l) adjusted to pH 3.5 with trifluoroacetic acid (20 mmol/l) (55:45, V/V)) at a flow rate of 1.2 ml/min. The detection wavelength was set at 320 nm. The calibration curve was linear (r = 0.9999) in the studied range of concentration (0.2-10 μg/ml). The selectivity and sensitivity of the elaborated method were satisfactory, and the limits of detection and quantification was less than 20% of the specification level. Moreover, the inter-and intra-day precisions was found to be less than 3% (RSD), while the recovery values expressing inter-and intra-day accuracy was varied from 99.73 to 101.23. The varenicline solution was stable over a period of 3 days on storage under refrigeration. The utility of the developed method was examined by analysing the tablets containing VAR. As a result, the method was found to be selective, sensitive, precise and accurate.

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Simultaneous determination of cotinine and trans‐3‐hydroxycotinine in urine by automated solid‐phase extraction using gas chromatography–mass spectrometry

Chiadmi

Schlatter

2014

Biomedical Chromatography

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A gas chromatography–mass spectrometry method was developed and validated for the simultaneous automated solid-phase extraction and quantification of cotinine and trans-3-hydroxycotinine in human urine. Good linearity was observed over the concentration ranges studied (R2 > 0.99). The limit of quantification was 10 ng/mL for both analytes. The limits of detection were 0.06 ng/mL for cotinine (COT) and 0.02 ng/mL for trans-3-hydroxycotinine (OH-COT). Accuracy for COT ranged from 0.98 to 5.28% and the precision ranged from 1.24 to 8.78%. Accuracy for OH-COT ranged from −2.66 to 3.72% and the precision ranged from 3.15 to 7.07%. Mean recoveries for cotinine and trans-3-hydroxycotinine ranged from 77.7 to 89.1%, and from 75.4 to 90.2%, respectively. This analytical method for the simultaneous measurement of cotinine and trans-3-hydroxycotinine in urine will be used to monitor tobacco smoking in pregnant women and will permit the usefulness of trans-3-hydroxycotinine as a specific biomarker of tobacco exposure to be determined. © 2014 The Authors. Biomedical Chromatography published by John Wiley & Sons Ltd.

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Quantification of nicotine, cotinine, trans-3′-hydroxycotinine and varenicline in human plasma by a sensitive and specific UPLC–tandem mass-spectrometry procedure for a clinical study on smoking cessation

Cited by 38 publications

References 43 publications

An isocratic liquid chromatography-electrospray ionization tandem mass spectrometric determination of varenicline in human plasma and dosage form

An isocratic liquid chromatography-electrospray ionization tandem mass spectrometric determination of varenicline in human plasma and dosage form

Development and validation of the HPLC method for varenicline determination in pharmaceutical preparation

Simultaneous determination of cotinine and trans‐3‐hydroxycotinine in urine by automated solid‐phase extraction using gas chromatography–mass spectrometry

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