Quantification of moxifloxacin in urine using surface-enhanced Raman spectroscopy (SERS) and multivariate curve resolution on a nanostructured gold surface

Abstract

A simple procedure is proposed for the determination of the antibiotic moxifloxacin in urine using nanostructured gold as surface-enhanced Raman scattering signal enhancer. The standard addition method in conjunction to multivariate curve resolution-alternating least squares was applied to eliminate the matrix effect and to isolate the spectral contribution of the analyte. Even in the presence of unexpected interferences in the urinary media, it was possible to extract and quantify the analyte response, reaching, in this way, the so-called second-order advantage from first-order data. Moreover, although a saturation phenomenon of the metallic surface was observed, the results of the proposed methodology presented important advantages such as high sensitivity and simpler experimental procedures. The moxifloxacin was determined at levels of 0.70 and 1.50 μg mL(-1) in urine diluted to 1.0% (corresponding to 70.0 and 150 μg mL(-1) in the original samples) with relative errors of 4.23 and 8.70%, respectively. The limit of detection (0.085 μg mL(-1)) and limit of quantification (0.26 μg mL(-1)) values indicated that the quantification can be accomplished in urine up to 24 h after the administration of a single 400-mg dose.

Application of SERS spectroscopy for detection of trace components in urinary deposits

“…Consequently, ways to overcome these issues should be sought. Urine and its constituents have been analyzed by SERS spectroscopy on more than one occasion [32,33]. The information about the components of interest was retrieved by applying mathematical procedure -multivariate curve resolution-alternating least squares (MCR-ALS) algorithm.…”

Quantification of Pharmaceutical Compounds Based on Powder X-Ray Diffraction with Chemometrics

“…Marqués 24) studied the acid-base behavior of adenine, adenosine 3,5-cyclic monophosphate and poly(adenylic) acid at 37°C by a spectrophotometric titration in acetonitrile-water mixtures. Nigam et al 25) used MCR-ALS and ultraviolet 26) adopted standard addition method in conjunction with MCR-ALS in order to eliminate the matrix effect and to isolate the spectral contribution of analyte. They used nanostructured gold as a surface-enhanced Raman scattering signal enhancer for kinetic analysis.…”

On-line microextraction of moxifloxacin using Fe3O4 nanoparticle-packed in-tube SPME

“…5b shows typical chromatogram obtained after in-tube SPME of spiked urine sample (Urine 1), which appearance of new sharp peak of the drug is obvious in spiked urine sample. Mamian-Lopez and Poppi reported that moxifloxacin is in the range of 700 to 1500 µg mL -1 in urine 22 . By our method these concentration can be easily detected.…”