Quantification of mesna and total mesna in kidney tissue by high-performance liquid chromatography with electrochemical detection

Verschraagen, Miranda; Bosma, Marjolein; Zwiers, T.H.Ursula; Torun, Emine; Vijgh, W.J.F. van der

doi:10.1016/s1570-0232(02)00470-1

Cited by 22 publications

(36 citation statements)

References 16 publications

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“…6,8 Also, the stability of mesna and total mesna in tissue during sample processing, storage, and tray stability has been described. 9 Therefore, only the stability of mesna and BNP7787 in kidney tissue during various storage conditions was investigated here with the dual ECD. The mesna and total mesna concentrations were also determined by HPLC with single ECD as described previously.…”

Section: Stability Of Mesna and Bnp7787 In Tissuementioning

confidence: 99%

“…After 45 min, the kidneys were removed and immediately frozen after washing in cold PBS solution to remove the blood. Tissue homogenate and deproteinized tissue homogenate of the kidneys were prepared as described before 9 and stored in aliquots at À20 and À808C. To determine the stability under these storage conditions, mesna and BNP7787 concentrations were determined in duplicate at day 0 and at different times up to 12 weeks of storage.…”

Section: Stability Of Mesna and Bnp7787 In Tissuementioning

confidence: 99%

“…The same result was found for BNP7787. The total mesna concentration, which was measured after precolumn reduction with sodium borohydride by HPLC with single ECD, 9 was also stable. The total mesna concentration (i.e., 13.4 mmol/g) was comparable to [mesna] þ 2 Â [BNP7787] (i.e., 7.7 mmol/g þ 2 Â 2.9 mmol/g), indicating that no mixed mesna disulfides were present in the kidney of a rat 45 min after an iv bolus injection of BNP7787 at 1000 mg/kg.…”

Section: Stability Of Mesna and Bnp7787 In Tissuementioning

confidence: 99%

“…[5][6][7][8][9] BNP7787 (the symmetrical disulfide of mesna) is measured indirectly as mesna after precolumn reduction with sodium borohydride. With this procedure, mixed disulfides with mesna (e.g., mesnacysteine, mesna-glutathione) will also contribute to the total amount of mesna generated by sodium borohydride.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we described HPLC procedures for the determination of the thiol mesna in plasma, urine, and tissue 8,9 using a sensitive and easy to maintain wall-jet gold electrode. Therefore, our objective was to use this type of electrode in a dual electrochemical detection system for developing and validating a procedure for the simultaneous determination of BNP7787 and mesna in plasma and tissue after separation by micro-HPLC.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of BNP7787 and its metabolite mesna in plasma and tissue by micro-HPLC with a dual electrochemical detector

Verschraagen

Zwiers

Torun

et al. 2003

Journal of Pharmaceutical Sciences

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Section: Stability Of Mesna and Bnp7787 In Tissuementioning

confidence: 99%

Section: Stability Of Mesna and Bnp7787 In Tissuementioning

confidence: 99%

Section: Stability Of Mesna and Bnp7787 In Tissuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous determination of BNP7787 and its metabolite mesna in plasma and tissue by micro-HPLC with a dual electrochemical detector

Verschraagen

Zwiers

Torun

et al. 2003

Journal of Pharmaceutical Sciences

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Boyce

2003

J. Am. Soc. Inf. Sci.

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High performance liquid chromatography analysis of MESNA (2-mercaptoethane sulfonate) in biological samples using ?uorescence detection

Mare

Penugonda

Erçal

2005

Biomed. Chromatogr.

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MESNA is the sodium salt of 2-mercaptoethane sulfonate, a thiol-containing drug. It is an antioxidant used particularly in renal protection. Several studies have proved that MESNA has beneficial effects in ischemic acute renal failure where it scavenges reactive oxygen species (ROS), due to the presence of the thiol group. It also reduces the size of urinary bladder cancer. MESNA was proved to be effective in preventing hemorrhagic cystitis induced by high doses of several chemotherapeutic regimens such as cyclophosphamide and ifosfamide. It has been shown that MESNA functions as an uroprotective substance in drug-induced experimental bladder cancer models. Moreover, recent studies have suggested that it is also effective in reducing intestinal inflammation in colitis. Because of the increased level of interest in using MESNA for treating various disorders, a new and sensitive method was needed to understand the pharmacokinetics of this drug. Accordingly, we developed a new method for determining free MESNA in biological samples by using ThioGlo-3 [3H-Naphto [2,1-b] pyran, 9-acetoxy-2-(4-(2,5-dihydro-2, 5-dioxo-1H-pyrrol-1-yl) phenyl-3-oxo)] as the derivatizing agent. MESNA was detected fluorimetrically by reverse-phase HPLC using acetonitrile:water (75:25) along with acetic acid and phosphoric acid (1 mL/L each) as the mobile phase. The detection limit was 1.64 nm per 20 µL injection volume, with a linearity (r = 0.999) in the calibration curve extending over a range 2.5-2500 nm. The coefficients of variation for within-run and between-run precision were 0.43 and 3.31%, respectively. The relative recoveries in the biological samples were in the range 87 ± 6 to 93 ± 2.4%. The concentrations of MESNA in the biological samples (lungs, liver, kidney and brain) were determined. The highest concentration of MESNA was found in plasma. Of all the tissues, the kidney was found to have the highest concentration while the liver had the lowest concentration. Abbreviations used: MESNA, 2-mercaptoethane sulfonate; ROS, reactive oxygen species; SBB, serine borate buffer.

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Quantification of mesna and total mesna in kidney tissue by high-performance liquid chromatography with electrochemical detection

Cited by 22 publications

References 16 publications

Simultaneous determination of BNP7787 and its metabolite mesna in plasma and tissue by micro-HPLC with a dual electrochemical detector

Simultaneous determination of BNP7787 and its metabolite mesna in plasma and tissue by micro-HPLC with a dual electrochemical detector

In this issue

High performance liquid chromatography analysis of MESNA (2-mercaptoethane sulfonate) in biological samples using ?uorescence detection

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