Simultaneous determination of BNP7787 and its metabolite mesna in plasma and tissue by micro-HPLC with a dual electrochemical detector

Verschraagen, Miranda; Zwiers, T.H.Ursula; Torun, Emine; Donker, Martin G.; Reinhoud, N.J.; Vijgh, W.J.F. van der

doi:10.1002/jps.10363

Cited by 12 publications

(11 citation statements)

References 15 publications

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“…Electrochemical HPLC (HPLC-EC) approaches provide a sensitive, derivatization-free approach to detecting thiols and disulfides in biological fluids and have been used in the detection of the BNP7787 metabolite, mesna, but often require mercury electrodes [21][22][23][24][25][26][27][28][29]. Recently, an HPLC-EC method employing an Ag/AgCl reference electrode was used to measure BNP7787 indirectly (as mesna) after a pre-column reduction using sodium borohydride [26].…”

Section: Introductionmentioning

confidence: 99%

Analysis of BNP7787 thiol-disulfide exchange reactions in phosphate buffer and human plasma using microscale electrochemical high performance liquid chromatography

Shanmugarajah

Ding

Huang

et al. 2009

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Analysis of BNP7787 thiol-disulfide exchange reactions in phosphate buffer and human plasma using microscale electrochemical high performance liquid chromatography

Shanmugarajah

Ding

Huang

et al. 2009

Journal of Chromatography B

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“…9,21 The chromatograms of selected concentrations of mesna (0.01-50 :M) diluted in the untreated cell lysate solution at 37 C were used to generate a standard curve. Peak area increased with increasing mesna concentrations.…”

Section: Hplc Analysismentioning

confidence: 99%

Accumulation of BNP7787 in Human Renal Proximal Tubule Cells

Hausheer

Ding

Shanmugarajah

et al. 2011

Journal of Pharmaceutical Sciences

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“…BNP7787 and mesna concentrations were determined selectively in one run using micro-HPLC with a dual electrochemical detector (dual ECD) (Verschraagen et al, 2003b). Briefly, citrate-NaOH buffer (40 ml of a mixture of 1 ml 5.0 M sodium hydroxide and 4 ml 0.5 M tri-sodium citrate buffer, pH 3.0) was added to 200 ml of deproteinised plasma, RBC or tissue sample, and the sample was injected into the micro-HPLC system which consisted of a Phenomenex C18 guard column (10 mm, 4 Â 2.0 mm) and a Phenomenex Prodigy C18 ODS-3 column (5 mm, 100 Â 1.0 mm; Bester, Amstelveen, The Netherlands).…”

Section: Sample Analysismentioning

confidence: 99%

Pharmacokinetic behaviour of the chemoprotectants BNP7787 and mesna after an i.v. bolus injection in rats

et al. 2004

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In preclinical studies, BNP7787 (disodium 2,2 0 -dithio-bis-ethane sulphonate), the disulphide form of mesna, has demonstrated selective protection against cisplatin-induced nephrotoxicity due to conversion into mesna inactivating toxic platinum species. Mesna (sodium 2-mercapto ethane sulphonate), however, can affect the antitumour activity of cisplatin, while BNP7787 does not interfere with the antitumour activity. To understand the difference in interference with cisplatin-induced antitumour activity between BNP7787 and mesna as well to characterise the selective nephroprotection by BNP7787, the pharmacokinetics of BNP7787 and mesna, each given i.v. 1000 mg kg À1 , were determined in plasma, kidney, liver, red blood cells (RBC), skeletal muscle and tumour of Fischer rats bearing subcutaneously implanted WARD colon tumours. The following results were obtained: (1) high concentrations of BNP7787 and mesna were observed in the plasma and kidney after administration of BNP7787 or mesna, except for mesna in plasma after BNP7787 administration; (2) in all other sampled compartments, the AUC values of both compounds were at least 5.5-fold lower than the corresponding values in kidney; (3) the AUC of mesna in plasma after mesna administration was comparable to the AUC of mesna in kidney after a dose of BNP7787 that can completely prevent cisplatin-induced nephrotoxicity in rats; (4) the AUC of mesna in plasma was five-fold higher relative to the AUC of mesna following BNP7787 administration (Po0.01). In conclusion, the five-fold higher AUC of mesna in plasma after mesna administration and the fact that mesna is more reactive with (hydrated) cisplatin than its disulphide form BNP7787 represent a plausible explanation as to why mesna administration can reduce the antitumour activity of cisplatin. After BNP7787 administration, the distribution of BNP7787 and mesna was restricted to the kidney, which confirmed the selective protection of the kidney by BNP7787.

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Simultaneous determination of BNP7787 and its metabolite mesna in plasma and tissue by micro-HPLC with a dual electrochemical detector

Cited by 12 publications

References 15 publications

Analysis of BNP7787 thiol-disulfide exchange reactions in phosphate buffer and human plasma using microscale electrochemical high performance liquid chromatography

Analysis of BNP7787 thiol-disulfide exchange reactions in phosphate buffer and human plasma using microscale electrochemical high performance liquid chromatography

Accumulation of BNP7787 in Human Renal Proximal Tubule Cells

Pharmacokinetic behaviour of the chemoprotectants BNP7787 and mesna after an i.v. bolus injection in rats

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