Quantification of major phenolic and flavonoid markers in forage crop Lolium multiflorum using HPLC-DAD

Kuppusamy, Palaniselvam; Lee, Kyung Dong; Song, Chae Eun; Ilavenil, Soundharrajan; Srigopalram, Srisesharam; Arasu, Mariadhas Valan; Choi, Ki Choon

doi:10.1016/j.bjp.2018.03.006

Cited by 41 publications

(31 citation statements)

References 34 publications

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“…The standards of phenolic compounds, organic acids and individual carbohydrates were determined based on the retention times and quantified based on their calibration curves (all the curves had R 2 higher than 0.98). For samples, the limits of detection (LOD) and limits of quantification (LOQ) were calculated according to Kuppusamy et al (2018) [36][37][38].…”

Section: Hplc Instrumentation and Separation Conditionsmentioning

confidence: 99%

Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws

Ursachi

Gutt

2020

Applied Sciences

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The aim of this study is to find the optimal pretreatment conditions and hydrolysis in order to obtain a high yield of bioethanol from wheat straw. The pretreatments were performed with different concentrations of sulphuric acid 1, 2 and 3% (v/v), and were followed by an enzymatic hydrolysis that was performed by varying the solid-to-liquid ratio (1/20, 1/25 and 1/30 g/mL) and the enzyme dose (30/30 µL/g, 60/60 µL/g and 90/90 µL/g Viscozyme® L/Celluclast® 1.5 L). This mix of enzymes was used for the first time in the hydrolysis process of wheat straws which was previously pretreated with dilute sulfuric acid. Scanning electron microscopy indicated significant differences in the structural composition of the samples because of the pretreatment with H2SO4 at different concentrations, and ATR-FTIR analysis highlighted the changes in the chemical composition in the pretreated wheat straw as compared to the untreated one. HPLC-RID was used to identify and quantify the carbohydrates content resulted from enzymatic hydrolysis to evaluate the potential of using wheat straws as a raw material for production of cellulosic ethanol in Romania. The highest degradation of lignocellulosic material was obtained in the case of pretreatment with 3% H2SO4 (v/v), a solid-to-liquid ratio of 1/30 and an enzyme dose of 90/90 µL/g. Simultaneous saccharification and fermentation were performed using Saccharomyces cerevisiae yeast, and for monitoring the fermentation process a BlueSens equipment was used provided with ethanol, O2 and CO2 cap sensors mounted on the fermentation flasks. The highest concentration of bioethanol was obtained after 48 h of fermentation and it reached 1.20% (v/v).

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Section: Hplc Instrumentation and Separation Conditionsmentioning

confidence: 99%

Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws

Ursachi

Gutt

2020

Applied Sciences

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“…Liquid chromatography (LC) coupled with diode array detection (DAD) or mass spectrometry (MS) has been used to quantify polyphenols. Several studies have developed LC‐DAD methods to quantify a number of polyphenols in plant extracts, since UV detection offers some selectivity based on the retention time and absorbance of a particular analyte in a sample; however, LC‐DAD has low selectivity with complex samples that contain mixtures of co‐eluting analytes. Alternatively, LC‐MS has been widely applied in the analysis of complex polyphenolic mixtures due to its higher sensitivity and selectivity .…”

Section: Introductionmentioning

confidence: 99%

Polyphenol profile comparisons of seed coats of five pulse crops using a semi‐quantitative liquid chromatography‐mass spectrometric method

Elessawy

Bazghaleh

Vandenberg

et al. 2019

Phytochemical Analysis

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Pulse crops are nutritious, but their seed coats are typically discarded, despite their high content of polyphenols. Polyphenol diversity among seed coats of five pulse crops was examined using a 30‐min liquid chromatography‐mass spectrometry (LC‐MS) method. Notable differences within polyphenol classes were observed among crops and genotypes. Polyphenol profiling is the first step in understanding how pulse crop seed coats can be better utilised and also in ultimately determining which polyphenols and biochemical pathways are important for human health benefits.

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“…Flavonoids have been reported to be an important constituent of medicinal plants, flavonoids have been assayed for their antioxidant activity 15,16 . Various techniques have been employed for the quantification of flavonoids which include advanced techniques like HPLC RP-HPLC, microwave-assisted extraction 17,18 . The active principles in the plants may not be major compound(s) in it but the activity of a compound may be masked by some other components in an extract 20,21 .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2020

IJPSR

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Quantification of major phenolic and flavonoid markers in forage crop Lolium multiflorum using HPLC-DAD

Cited by 41 publications

References 34 publications

Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws

Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws

Polyphenol profile comparisons of seed coats of five pulse crops using a semi‐quantitative liquid chromatography‐mass spectrometric method

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