Quantification of intercellular adhesion forces measured by fluid force microscopy

Cohen, Noa; Sarkar, Saheli; Hondroulis, Evangelia; Sabhachandani, Pooja; Konry, Tania

doi:10.1016/j.talanta.2017.06.038

Cited by 17 publications

(23 citation statements)

References 34 publications

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“…By applying an underpressure relative to the environment, it is possible to draw a cell towards the opening of the cantilever and retain it there. The FluidFM has been used to study the elasticity and adhesion of several cell types [15][16][17][18]. Studies addressing specifically the adhesion of bacterial cells include the work of Potthoff et al [19], Sprecher et al [20] and Mittelviefhaus et al [21].…”

Section: Introductionmentioning

confidence: 99%

FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy

2020

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The FluidFM enables the immobilization of single cells on a hollow cantilever using relative underpressure. In this study, we systematically optimize versatile measurement parameters (setpoint, z-speed, z-length, pause time, and relative underpressure) to improve the quality of force-distance curves recorded with a FluidFM. Using single bacterial cells (here the gram negative seawater bacterium Paracoccus seriniphilus and the gram positive bacterium Lactococcus lactis), we show that Single Cell Force Spectroscopy experiments with the FluidFM lead to comparable results to a conventional Single Cell Force Spectroscopy approach using polydopamine for chemical fixation of a bacterial cell on a tipless cantilever. Even for the bacterium Lactococcus lactis, which is difficult to immobilze chemically (like seen in an earlier study), immobilization and the measurement of force-distance curves are possible by using the FluidFM technology.

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Section: Introductionmentioning

confidence: 99%

FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy

2020

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“…[30] Cohen et al used fibronectin and collagen coating to measure the adhesion force of mammalian cells. [18] Note that sometimes the adsorption of the targeted cells may not be sufficient. This can be reflected in the force curve while performing the detachment experiment.…”

Section: Substrate Preparationmentioning

confidence: 99%

“…For example, while measuring the intercellular adhesion forces of mammalian cells, a cantilever with a large opening (generally 8 µm) was used. [ 18 ] To reversibly attach Cryptosporidium oocysts for measuring deformability, a cantilever with an aperture diameter of either 2 or 4 µm was used. [ 19 ] For tipless cantilevers, the opening is a hollow circular aperture.…”

Section: Fluidfm Set‐up and Cantilever Preparationmentioning

confidence: 99%

Fundamentals and Applications of FluidFM Technology in Single‐Cell Studies

Saha

Duanis‐Assaf

Reches

2020

Adv Materials Inter

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various microfluidic devices have been routinely employed. However, in these approaches, there is either a high chance of damaging the cells while detaching the adherent cells from the surfaces, or isolation of individual cells from the adherent cell culture is not at all possible. Table 1 lists the drawbacks and challenges associated with these cell sorting techniques. Recently, atomic force microscope (AFM) has emerged as a significant tool to study microorganisms and mammalian cells in real time. This is due to its compatibility with the physiological conditions of the cells. [3] AFM allows the bioimaging of cells with various degrees of resolution, from whole cells [4] down to individual molecules. [5] Using force-curve based imaging, Alsteens et al. revealed single bacteriophages extruding from the surface of bacteria, [6] whereas our group was able to image nanostructures termed microvilli on the surface of T cells. [7] With the introduction of high-speed AFMs, Yamashita et al. were able to follow the diffusion of single molecules on the surface of living bacterial cells in real time. [8] Valuable biophysical and structural information can be achieved down to a single-cell level by means of high-resolution imaging and force spectroscopy. [9] In force spectroscopy experiments using AFM, a cell is directly attached to the cantilever apex with a biocompatible glue (e.g., polydopamine (PDA)). However, since the cell is irreversibly bound to the AFM probe, each functionalized cantilever can be utilized with only one cell for manipulation. It is, therefore, challenging to perform serial and rapid measurement of a reasonable number of cells; this makes it a time-consuming and elaborate process to address a statistical distribution. For injecting biomolecules such as dyes, enzymes or nanoparticles into single living cells, various approaches such as nano-fountain probes, [10] nanoneedles, [11] and carbon nanotubes [12] have been used. However, these approaches lack precise handling for local dispensing of fluid and are limited to the handling of very small volumes (on the order of attoliters (aLs) up to 50 femtoliters (fLs)). Moreover, using these approaches, a relatively long period of time is required to perform intracellular injection. With the advancement of technologies, it has become relatively easier to address these problems in a more controlled manner. Conventional AFM has been integrated with microfluidics to overcome these limitations. [13] This bioanalytical tool is known as a fluidic force microscope or FluidFM, which was This review describes the potential of FluidFM technology and its implementation in studying the interface between a single cell (prokaryote or eukaryote) and a surface or a surrounding area. A combination of microfluidics with conventional atomic force microscope (AFM) makes this platform efficient to address challenges associated with various biomolecular systems and biophysical activities down to single-cell levels. Upon regulating the pressure through a microchanneled cantilever v...

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“…Cell adhesion forces were measured using short (contact duration: 1–50 s) and long-term (contact duration: 30–60 min) adhesion protocols. At short contact duration, the results reported similar adhesion forces in homotypic and heterotypic conditions, while they differ at longer contact period [ 37 ].…”

Section: The Fluidfm Technologymentioning

confidence: 99%

AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology

2018

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Atomic force microscopy (AFM) is a widely used imaging technique in material sciences. After becoming a standard surface-imaging tool, AFM has been proven to be useful in addressing several biological issues such as the characterization of cell organelles, quantification of DNA-protein interactions, cell adhesion forces, and electromechanical properties of living cells. AFM technique has undergone many successful improvements since its invention, including fluidic force microscopy (FluidFM), which combines conventional AFM with microchanneled cantilevers for local liquid dispensing. This technology permitted to overcome challenges linked to single-cell analyses. Indeed, FluidFM allows isolation and injection of single cells, force-controlled patch clamping of beating cardiac cells, serial weighting of micro-objects, and single-cell extraction for molecular analyses. This work aims to review the recent studies of AFM implementation in molecular and cellular biology.

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Quantification of intercellular adhesion forces measured by fluid force microscopy

Cited by 17 publications

References 34 publications

FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy

FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy

Fundamentals and Applications of FluidFM Technology in Single‐Cell Studies

AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology

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