Quantification of Glutathione in Plasma Samples by HPLC Using 4-Fluoro-7-nitrobenzofurazan as a Fluorescent Labeling Reagent

Wang, Xifeng; Chi, Defeng; Song, Dajun; Su, Guosheng; Lin, Li; Shao, Lingyun

doi:10.1093/chromsci/bmr039

Cited by 36 publications

(16 citation statements)

References 20 publications

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“…In vivo, the liver is the major source of GSH in the circulation (55,88). GSH levels in human plasma of healthy adults were estimated to be 2-5 lM and the GSSG concentration was found to be much lower (0.14 lM) (40,66,101). Intriguingly, plasma GSH levels declined in the elderly and in diabetic patients with a corresponding rise in GSSG levels (85), indicating a shift to a more oxidizing state in aging and diabetes.…”

Section: Cellular Distribution Of the Gsh/gssg Couplementioning

confidence: 99%

Metabolic Responses to Reductive Stress

Xiao

Loscalzo

2020

Antioxidants & Redox Signaling

267

187

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Significance: Reducing equivalents (NAD(P)H and glutathione [GSH]) are essential for maintaining cellular redox homeostasis and for modulating cellular metabolism. Reductive stress induced by excessive levels of reduced NAD + (NADH), reduced NADP + (NADPH), and GSH is as harmful as oxidative stress and is implicated in many pathological processes. Recent Advances: Reductive stress broadens our view of the importance of cellular redox homeostasis and the influences of an imbalanced redox niche on biological functions, including cell metabolism. Critical Issues: The distribution of cellular NAD(H), NADP(H), and GSH/GSH disulfide is highly compartmentalized. Understanding how cells coordinate different pools of redox couples under unstressed and stressed conditions is critical for a comprehensive view of redox homeostasis and stress. It is also critical to explore the underlying mechanisms of reductive stress and its biological consequences, including effects on energy metabolism. Future Directions: Future studies are needed to investigate how reductive stress affects cell metabolism and how cells adapt their metabolism to reductive stress. Whether or not NADH shuttles and mitochondrial nicotinamide nucleotide transhydrogenase enzyme can regulate hypoxia-induced reductive stress is also a worthy pursuit. Developing strategies (e.g., antireductant approaches) to counteract reductive stress and its related adverse biological consequences also requires extensive future efforts. Antioxid. Redox Signal. 32, 1330-1347.

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Section: Cellular Distribution Of the Gsh/gssg Couplementioning

confidence: 99%

Metabolic Responses to Reductive Stress

Xiao

Loscalzo

2020

Antioxidants & Redox Signaling

267

187

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“…Various methods could be used for glutathione determinations in physiological fluids, including high pressure liquid chromatography (HPLC) with different kind of detectors: electrochemical [44][45][46], UV [47][48][49], fluorometric [50,51], mass spectrometer [52,53], gas chromatography with mass spectrometry [54,55]. HPLC methods are the ones most frequently applied because of their specificity and sensitivity.…”

Section: Methodsmentioning

confidence: 99%

The Influence of Crenotheraphy with SHS Water on the Concentration of Glutathione in Blood of People with Rheumatoid Arthritis

Błońska-Sikora¹,

Orlewska²,

Klusek³

2018

Preprint

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The tripeptide-glutathione (GSH, γ-L-glutamyl-L-cysteinyl-glycine) is one of the most important low molecular antioxidant in human body. Enhancing GSH and associated enzymes represents an aim in the search for cytoprotective strategies against cancer, neurologic degeneration, pulmonary and inflammatory conditions, as well as rheumatoid arthritis (RA).The objective of the study was to agree whether crenotherapy (drinking therapy) with sulfide/hydrogen sulfide (SHS) waters from “Zuzanna” spring located in the area of Busko-Zdrój in Poland leads to increasing of reduced glutathione (GSH) content in human blood. SHS water in distinct from mineral water is characterised by specific pharmacokinetic, invariable content and natural microbiological purity. SHS waters contain at least 1 g of total sulfur per kilogram of water and a treatment effect also depends on other bioelements. The method employing capillary electrophoresis with UV detector for the analysis of glutathione in human blood was developed. The group of 106 volunteers consisted of both women and men, in different age range. The therapy with SHS waters lasted 2 weeks. We recently demonstrated that the administration of hydrogen sulfide (H2S) in SHS waters increases GSH concentration in blood, and therefore crenotherapy could be used in therapeutics.

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“…Milder reaction conditions (10 min at 30°C) were attained by Wang et al, via using 4-fluoro-7nitrobenzofurazan (NBD-F). Despite the favorable reaction conditions, the product was unstable as evidenced by the decrease in peak area with increased column temperature or storage at −20°C for more than 72 h (Wang et al, 2012).…”

Section: 25%mentioning

confidence: 99%

“…A low concentration in the nm level was reached by Michaelsen et al (2009) through using ophthaldialdehyde (OPA) as a derivatizing agent and the thiol-reducing agent: NAC ethyl ester (NACET). Although NACET is a strong and specific disulfide-reducing agent, OPA is nonspecific for thiol groups, and GSH-OPA is unstable, which affects the method accuracy and precision (Wang et al, 2012). The most sensitive RPLC/FL method was presented by Guo et al (2009) by using 1,3,5,7-tetramethyl-8-phenyl-(4iodoacetamido)difluoroboradiaza-s-indacene (TMPAB-I).…”

Section: 25%mentioning

confidence: 99%

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Hamad

Elshahawy

Negm

et al. 2020

Reviews in Analytical Chemistry

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AbstractGlutathione is a natural tripeptide that plays a major role in different physiological processes in the human body. Determination of glutathione in different body fluids and tissues is essential for early diagnosis and follow-up of various diseases. The assay of glutathione is problematic because of the high polarity, the limited stability, and the aliphatic structure, which lacks the appropriate chromophore for UV detection. A number of methods have been reported for determination of glutathione using different techniques. High-performance liquid chromatography was employed in both reverse phase and hydrophilic interaction modes. Electrochemical methods exploited the redox activity of glutathione to allow for quantification by different electrodes after chemical modification, including glassy carbon, carbon paste, and nanocomposite electrodes. Capillary zone electrophoresis was used with less need for derivatization which makes it simpler, faster, and more economic. A number of nanosensors and probes have been developed to assay glutathione in biological fluids using semiconductor nanoparticles, quantum dots, genetically engineered green fluorescent probes, and new derivatives of known dye classes. This work is an updated review of the methods of analysis of glutathione and glutathione disulfide in pharmaceuticals and biological fluids with more emphasis on the technical problems and the assay artifacts.

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Quantification of Glutathione in Plasma Samples by HPLC Using 4-Fluoro-7-nitrobenzofurazan as a Fluorescent Labeling Reagent

Cited by 36 publications

References 20 publications

Metabolic Responses to Reductive Stress

Metabolic Responses to Reductive Stress

The Influence of Crenotheraphy with SHS Water on the Concentration of Glutathione in Blood of People with Rheumatoid Arthritis

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

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