Quantification of glucose diffusion in arterial tissues by using optical coherence tomography

Larin, Kirill V.; Ghosn, Mohamad G.; Ivers, Steven; Téllez, Armando; Granada, Juan F.

doi:10.1002/lapl.200610111

Cited by 68 publications

(46 citation statements)

References 54 publications

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“…Recently we demonstrated the capability of OCT for noninvasive and nondestructive monitoring and quantifying of the permeability coefficient of different analytes and drugs in various biological tissues. [6][7][8][9] In this letter, we report results on quantification of diffusion of biomolecules in normal and diseased areas of pig aorta. The diagnosis from information provided by OCT could help in timely and effective treatment.…”

Section: Introductionmentioning

confidence: 99%

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

et al. 2008

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Abstract. Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography ͑OCT͒ is an ideal diagnostic tool due to the high resolution this technology achieves in discriminating the different features of atherosclerotic lesions based on structural imaging. We explore the capability of OCT for functional imaging of normal and atherosclerotic aortic tissues based on time-and depth-resolved quantification of the permeability of biomolecules through these tissues. The permeability coefficient of 20% aqueous solution of glucose was found to be ͑6.80± 0.18͒ ϫ 10 −6 cm/ s in normal aortas and ͑2.69± 0.42͒ ϫ 10 −5 cm/ s in aortas with atherosclerotic disease. The results suggest that this new OCT functional imaging method-the assessment of the permeability coefficients of various physiologically neutral biomolecules in vascular tissues-could assist in early diagnosing and detecting the different components of atherosclerotic lesions.

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Section: Introductionmentioning

confidence: 99%

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

et al. 2008

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“…37,38 The permeability coefficient is obtained by analyzing the changes of OCTSS in a selected region during the diffusion process of hyperosmotic agents. A linear region with minimal fluctuation in the averaged OCT signal profile is selected and its physical thickness (ΔL) is measured (assuming the refractive index of 1.4).…”

Section: Methodsmentioning

confidence: 99%

Synergistic effect of hyperosmotic agents and sonophoresis on breast tissue optical properties and permeability studied with spectral domain optical coherence tomography

Zhu

Wei

et al. 2012

J. Biomed. Opt

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Abstract. Hyperosmotic agents have shown great potential in tissue optical clearing. However, the low efficiency of the permeation in biological tissues seriously restricts its application in reality. The synergy of sonophoresis as a penetration enhancer and hyperosmotic agents, 20% glucose (G) and 20% mannitol (M), in optical clearing has been investigated by analyzing the variation of the attenuation coefficients and the permeability coefficients. In the sonophoresis experiments, ultrasound (US) was applied for 10 min before applying hyperosmotic agents. Along with the administration of hyperosmotic agents, the samples were monitored with optical coherence tomography (OCT) functional imaging for the next 2 h. The attenuation coefficients of each group were obtained from the 2-D OCT images based on Beer's Law. The original attenuation coefficient is 12.38 AE 0.73 cm −1 in normal breast tissue. After 45 min treatment, it changes to be 5.91 AE 0.82 cm −1 and 4.14 AE 0.67 cm −1 for 20% G and 20% G/US, respectively. The attenuation coefficient of breast cancer tissue is 18.17 AE 1.45 cm −1 at the beginning, and it becomes 8.70 AE 0.87 cm −1 for 20% G and 6.80 AE 0.92 cm −1 for 20% G/US after 30 min. Meanwhile, the permeability coefficients of hyperosmotic agents were much enlarged by the treatment of ultrasound in both breast normal tissue and breast cancer tissue. A significant difference in permeability coefficients between health tissue and tumor tissue was also observed in the experiment (p < 0.01).

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“…The permeability coefficient of a hyperosmotic agent in a specific region of tissues ex vivo was calculated by the OCT signal slope (OCTSS) method, which was computed by analyzing the slope changes in the OCT signal caused by the hyperosmotic agent diffusion [45,46,57,62]. Due to the hyperosmotic agent diffusion in tissues, the light scattering coefficient decreased in the specific depth region, which can be clearly reflected from the OCTSS graphs [62].…”

Section: Methodsmentioning

confidence: 99%

Quantification of glycerol diffusion in human normal and cancer breast tissuesin vitrowith optical coherence tomography

et al. 2010

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Optical coherence tomography (OCT) holds great promise as a routine research tool for analysis of identifying the boundaries between normal and diseased breast tissue in vitro and in vivo. However, despite the depth penetration afforded by this imaging modality, light attenuation in tissues imposes limitations. Here we studied the optical clearing effect of glycerol in human cancer and normal breast tissues with OCT for functional imaging to monitor. Depth- and time-resolved profiles for OCT signal enhancement were presented. The results show that the OCT imaging depth and imaging contrast of breast tissues have been improved after application of 60% glycerol in the 2-D OCT images. The OCT slope signals of breast tissues decreased as glycerol diffusion into tissues, therefore, the water and intercellular fluids were drawn out from tissues. Then the reverse process due to water was drawn back into the cells as a result of its affinity for water. The permeability coefficient of 60% glycerol was (3.14±0.07)×10-5 cm/s in breast cancer tissues, and (0.89±0.02)×10-5 cm/s in normal breast tissues, respectively. The permeability coefficient of glycerol in cancer tissues was 3.54-fold than that in normal tissues. These results demonstrate that the optical clearing of normal and cancer breast tissues are improved after application of glycerol.

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Quantification of glucose diffusion in arterial tissues by using optical coherence tomography

Cited by 68 publications

References 54 publications

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

Synergistic effect of hyperosmotic agents and sonophoresis on breast tissue optical properties and permeability studied with spectral domain optical coherence tomography

Quantification of glycerol diffusion in human normal and cancer breast tissuesin vitrowith optical coherence tomography

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