Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

Abstract

Abstract. Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography ͑OCT͒ is an ideal diagnostic tool due to the high resolution this technology achieves in discriminating the different features of atherosclerotic lesions based on structural imaging. We explore the capability of OCT for functional imaging of normal and atherosclerotic aortic tissues based on time-and depth-resolved quantification of the permeability of biomolecules through these tissues. The permeability coefficient of 20% aqueous solution of glucose was found to be ͑6.80± 0.18͒ ϫ 10 −6 cm/ s in normal aortas and ͑2.69± 0.42͒ ϫ 10 −5 cm/ s in aortas with atherosclerotic disease. The results suggest that this new OCT functional imaging method-the assessment of the permeability coefficients of various physiologically neutral biomolecules in vascular tissues-could assist in early diagnosing and detecting the different components of atherosclerotic lesions.

Application of hyperosmotic agent to determine gastric cancer with optical coherence tomography ex vivo in mice

“…And the capability of OCT for noninvasive and nondestructive monitoring and quantifying of permeability coefficients of different analytes and drugs in various biological tissues was studied. 15,16 To explore the property of permeability coefficients of hyperosmotic agents in tumor tissues and improve the application of OCT in the diction of tumor tissues, we monitor and quantify the diffusion of 20% aqueous solution of glucose in tumor tissues. The results offer an effective method for clinical diagnosis and detection of tumor tissues.…”

Synergistic effect of hyperosmotic agents and sonophoresis on breast tissue optical properties and permeability studied with spectral domain optical coherence tomography

“…Based on the differences in permeability and optical property, OCT technology can be used to distinguish cancerous tissues from normal tissues. This method has been demonstrated by previous studies, [23][24][25] and has also been applied for breast cancer detection. 26,27 Glucose (G) is one of the most commonly used OCAs which have been widely used to investigating the permeability of biological tissues.…”

“…The difference in optical property between normal breast tissue and breast cancer tissue may be induced by the morphological and structural differences between the two types of tissues, such as larger nuclei, the higher nuclearto-cytoplasmic ratio in tumor cells, and the higher regional tumor cell density of the tumor tissues. 23 The attenuation coefficients of each group at different time were calculated from the data of the best exponential fit curve corresponding to the averaged intensity profiles. The selected region is from the depth of 110 μm (L 1 ) to the depth of 280 μm (L 2 ), where the OCT signal distribution is relatively smooth.…”

Quantitative assessment of hyaline cartilage elasticity during optical clearing using optical coherence elastography

“…Glucose is commonly used as a clearing agent due to its natural biocompatibility [3][4][5][6][7][8][9][10]. Currently, OCT structural images are widely utilized to monitor the process of optical clearing of samples via OCT signal slope and/or speckle variance analysis.…”