Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a human pathogen responsible for diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). To promote a comprehensive insight into the molecular basis of EHEC O157:H7 physiology and pathogenesis, the combined proteome of EHEC O157:H7 strains, Clade 8 and Clade 6 isolated from cattle in Argentina, and the standard EDL933 (clade 3) strain has been analyzed. From shotgun proteomic analysis a total of 2,644 non-redundant proteins of EHEC O157:H7 were identified, which correspond approximately 47% of the predicted proteome of this pathogen. Normalized spectrum abundance factor analysis was performed to estimate the protein abundance. According this analysis, 50 proteins were detected as the most abundant of EHEC O157:H7 proteome. COG analysis showed that the majority of the most abundant proteins are associated with translation processes. A KEGG enrichment analysis revealed that Glycolysis / Gluconeogenesis was the most significant pathway. On the other hand, the less abundant detected proteins are those related to DNA processes, cell respiration and prophage. Among the proteins that composed the Type III Secretion System, the most abundant protein was EspA. Altogether, the results show a subset of important proteins that contribute to physiology and pathogenicity of EHEC O157:H7.

Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of Enterotoxigenic Escherichia coli BE311 for Fluorescence Labeling and Enterotoxin Analyses

“…A study on enterohemorrhagic Escherichia coli (EHEC) O157:H7 has shown that the identified proteins were grouped into three important functional groups: metabolism, information storage and processing, cellular processes, and signaling [ 49 ]. Correspondingly, the BE311 genes were mainly annotated to cellular components and metabolism pathways ( Figure 2 A).…”

Characteristic Structures of Different Stilbenes Distinguish the Impact on Ochratoxin A Biosynthesis Intermediate Pathway and Metabolites of Aspergillus carbonarius

“…16 Reactive oxygen species (ROS) generation and their scavenging by antioxidant molecules, such as phenolic compounds, maintain a balance in the oxidative state. 17 The latter are well-known antioxidant metabolites, in part because their structures contain hydroxyl or methoxyl groups. 18 When grapes are used to make wine, their skin, seeds, and pomace contain significant levels of stilbenes, a type of phytoalexin (defensive compounds produced by plants).…”

“…One of the most important elements in controlling the development, conidial formation, and secondary metabolite biosynthesis in fungi is the proper redox balance . Reactive oxygen species (ROS) generation and their scavenging by antioxidant molecules, such as phenolic compounds, maintain a balance in the oxidative state . The latter are well-known antioxidant metabolites, in part because their structures contain hydroxyl or methoxyl groups …”

Preservation of protective capacity of hyperimmune anti-Stx2 bovine colostrum against enterohemorrhagic Escherichia coli O157:H7 pathogenicity after pasteurization and spray-drying processes

“…The EHEC O157:H7 expressing Stx2a/c are the most virulent microorganisms isolated from children with HUS (Rivas et al, 2014). Furthermore, most HUS patients are infected with EHEC O157:H7 of the hypervirulent clade 8, which overexpresses several virulence proteins including Stx2a/c (Da Silva et al, 2018).…”