Quantification of DNA in forensic samples

Nicklas, Janice A.; Buel, Eric

doi:10.1007/s00216-003-1924-z

Cited by 75 publications

(40 citation statements)

References 34 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diverse results in extraction of DNA among the individual strains and species of moulds were pointed out by Guo et al (2005). In our study, four methods were used to evaluate DNA concentration: spectrophotometric detection, fluorometric detection using PicoGreen compound (Ahn et al 1996;Haque et al 2003;Nicklas & Buel 2003), determination of the maximum amplifiable dilution of DNA (Olexová et al 2004), and semi-quantitative determination of the amount of DNA by staining with ethidium bromide and evaluation on agarose gel.…”

Section: Resultsmentioning

confidence: 90%

Comparison of methods for isolating fungal DNA

Moťková¹,

Vytřasová²

2011

Czech J. Food Sci.

View full text Add to dashboard Cite

Moťková P., Vytřasová J. (2011): Comparison of methods for isolating fungal DNA. Czech J. Food Sci., 29 (Special Issue): S76-S85.In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification before the proper DNA detection itself. For this purpose, classic methods of DNA extraction were compared and optimised while isolations using commercial kits were also done. The methods were evaluated from several perspectives, with focus especially laid on the isolated DNA not contain PCR inhibitors which would prevent DNA amplification, thus inhibiting the detection itself. For optimising the individual methods, collection strains of the genus Aspergillus were used. After the evaluation, two most suitable methods were selected and chosen for isolating potentially aflatoxigenic moulds taken from food samples. These methods were the commercially supplied kit for isolating DNA from plant leaves from Sigma and a classic method according Cenis in combination with the cell wall disruption by means of liquid nitrogen.

show abstract

Section: Resultsmentioning

confidence: 90%

Comparison of methods for isolating fungal DNA

Moťková¹,

Vytřasová²

2011

Czech J. Food Sci.

View full text Add to dashboard Cite

show abstract

“…In order to evaluate the extraction yields of methods, measure the quantity of DNA isolated from a known amount of source material. Pure DNA should have a ratio of approximately 1.8, rapid and efficient methods for the extraction of DNA specifically from bacterial cells in beef DNA with A260/280 nm ratio between 1.8 and 2.0 is considered pure [27], in recently study, the genomic DNA was high purity within a ratio of 1.5-2.…”

Section: Quantitymentioning

confidence: 96%

Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria

Abdelhai¹

2016

BIO

View full text Add to dashboard Cite

Abstract:Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the timeconsuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.

show abstract

“…A value of 260/280 OD ratio above 1.8 was generally accepted as pure DNA 26 . Ratios lowerthan 1.8 indicates the presence of protein contamination that was absorbed strongly at or near 280 nm 27,28 . Ratioshigher than 2.0 indicate the presence of RNA contamination 29 .…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Comparison of DNA Extraction Using Fresh and Stored Bloods in Molecular Hematology Diagnostic Study

Hassan

Mohamad

Hassan

et al. 2018

Bangladesh J Med Sci

View full text Add to dashboard Cite

Abstract:Objective: Blood is the main source of DNA in molecular biology. It provides a high DNA quality and quantity. In this study, we compare the quality and quantity of DNA isolated from stored blood that has been kept at -40°C for one-year to that of fresh blood. Materials and Methods: Twelve fresh and stored blood samples were randomly selected for this study. Nucleo Spin ® Blood L kit was used to isolate the DNA from the samples. The integrity and intensity of DNA were examined through 1.6% agarose gel precast with SYBR ® safe DNA stain. The DNA samples were further examined through PCR amplification and Sanger sequencing. Results: There was no significant difference in quality and quantity of isolated DNA from fresh blood and stored blood samples. The high intensity of an intact DNA band as well as the success in PCR amplification and sequencing are indicators of high DNA quality. Conclusion: Proper storage of patients'left-over whole blood sample at -40°C offers an acceptable alternative for DNA resources in molecular study.

show abstract

Quantification of DNA in forensic samples

Cited by 75 publications

References 34 publications

Comparison of methods for isolating fungal DNA

Comparison of methods for isolating fungal DNA

Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria

A Comprehensive Comparison of DNA Extraction Using Fresh and Stored Bloods in Molecular Hematology Diagnostic Study

Contact Info

Product

Resources

About