Quantification of Crystal Growth of Calcium Oxalate in Gel and its Modification by Urinary Constituents in a New Flow Model of Crystallization

Achilles, W.; Freitag, Rainer; Kiss, Béla; Riedmiller, H.

doi:10.1097/00005392-199510000-00091

Cited by 5 publications

(6 citation statements)

References 8 publications

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“…Urinary stones grow in a gel like medium; therefore, they have radially striated growth [16] . The crystal growth by gel method provides simulation of synovial cartilage and other biological fluids [17] . Gel growth (in vitro) of a few urinary stone constituents and the inhibitory role played by some extracts or juices of natural products in crystal growth were studied earlier [18] .…”

Section: Single Diffusion Gel Growth Techniquementioning

confidence: 99%

Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn

Chauhan¹

2009

American Journal of Infectious Diseases

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Problem statement: The formation of a urinary stone, known as nephrolithiasis, urolithiasis, renal calculi or kidney stone is a serious, debilitating problem in all societies throughout the world. Struvite or Ammonium Magnesium Phosphate Hexahydrate (AMPH) is one of the components of urinary stone (calculi). Struvite stones are commonly found in women. Struvites form in humans as a result of urinary tract infection with ureolithic urea splitting micro organisms. These stones can grow rapidly forming "staghorn-calculi", which is more painful urological disorder. Therefore, it is of prime importance to study the growth and inhibition of Struvite crystals. Approach: This in vitro study had been carried out in the presence of herbal extract of Boerhaavia diffusa Linn. by using single diffusion gel growth technique. Sodium metasilicate solution of specific gravity 1.05 and an aqueous solution of ammonium dihydrogen phosphate of 0.5 M concentration were mixed so that the pH value 7.0 could be set. After the gelation, equal amount of supernatant solution of 1.0 M magnesium acetate prepared with 0.5 and 1% concentrations of the herbal extract of B. diffusa Linn. were gently poured on the set gels in the respective test tubes in the aseptic medium. Results: The growth of crystals without and with herbal extracts was monitored at regular time intervals. As the concentration of B. diffusa Linn. increased, the inhibition of crystals also increased in the gel media as well as the dissolution of crystals at the gel-liquid interface increases. The de-fragmentation of some grown crystals was also noticed. Conclusion: The herbal extract of B. diffusa Linn. inhibited the growth of struvite crystals in vitro. This study incorporated multidisciplinary interests and may be used for formulating the strategy for prevention or dissolution of urinary stones.

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Section: Single Diffusion Gel Growth Techniquementioning

confidence: 99%

Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn

Chauhan¹

2009

American Journal of Infectious Diseases

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“…Depending on the special conditions applied, fixed crystal nucleation, growth and agglomeration, as well as effects upon these parts of crystallisation, can be directly observed by light microscopy. Crystal growth of CaOx could be quantified in this system by following scattered light as a function of time [4]. Phenomena of epitactic growth could be investigated by overgrowth of one crystal phase with another [5].…”

Section: Discussion On the Physiological Relevance Of In Vitro Crystamentioning

confidence: 99%

“…Nucleation rates, surprisingly, were significantly higher in normals than in stone patients. Achilles et al [4], applying the GCM described above, demonstrated a higher growth rate for CaOx in undiluted urine of male stone formers than for matched controls. Though this difference was clearly significant (P < 0.01), the overlap of single values from both groups led to the conclusion that factors additional to those affecting crystal growth are likely to be responsible, at least in part, for the genesis of CaOx urolithiasis.…”

Section: Relative Importance Of Parameters Of Crystallisation In Stonmentioning

confidence: 92%

“…This can be caused by interaction of the urinary components with the effector substance to be tested, by interaction between the constituents themselves (e.g., complex formation) and by binding phenomena at the crystal surface under scrutiny. Artificial urines composed of the most prominent lowmolecular-weight constituents of native urine have therefore been used as a reasonable compromise for testing and comparing the influence of various effectors on crystal formation [1,4,51]. However, it has long been the aim of investigators in the field of urolithiasis to adapt in vitro crystallisation systems to measurements with natural urine, predominantly for clinical and diagnostic use.…”

Section: Problems Connected With Test Solutions 247mentioning

confidence: 99%

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In vitro crystallisation systems for the study of urinary stone formation

Achilles¹

1997

World J Urol

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Various methods and techniques are aimed at modelling crystallisation processes of urinary stone formation in vitro. There are considerable differences between them in technical and physico-chemical principles, quantification of crystal nucleation, growth and agglomeration and the parameters measured. In this paper, some important in vitro systems are described as examples. They are compared with regard to some of their features and capabilities. Emphasis has been placed on evaluation of the physiological relevance of the methods. For that reason, the different in vitro models have been related to current views on intrarenal in vivo, mechanisms underlying stone formation and other independent experimental results. Crystallisation procedures carried out in aqueous solutions are likely to mimic crystalluria, corresponding to a free-particle mechanism. However, a specifically tailored flow technique of crystallisation in gels seems to be a reasonable model of stone formation, in accordance with the generally accepted fixed-particle theory.

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“…The case of insufficient intake of water or due to the decreased rate of excretion, the crystallogenic substances in the urine can be concentrated, leading to crystal formation. A change in the pH value in the urine can lead to the crystal growth (5,6). Humans are exposed to cadmium is if no use to the human body and is toxic even at low levels.…”

Section: Introduction:-mentioning

confidence: 99%

Growth, Structural, Morphological and Optical Characterizations of Pure and Cadmium Doped Calcium Oxalate Monohydrate Crystals.

Selvaraju¹,

Sulochana²

2017

IJAR

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Quantification of Crystal Growth of Calcium Oxalate in Gel and its Modification by Urinary Constituents in a New Flow Model of Crystallization

Abstract: Drinking therapy and lowering of urinary oxalate excretion can be concluded to be the most promising means of preventing CaOx urolithiasis. The new crystallization model demonstrated here should be suitable to new applications in the investigation of urinary stone formation.

Cited by 5 publications

References 8 publications

Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn

Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn

In vitro crystallisation systems for the study of urinary stone formation

Growth, Structural, Morphological and Optical Characterizations of Pure and Cadmium Doped Calcium Oxalate Monohydrate Crystals.

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