Quantification of cell-free DNA for evaluating genotoxic damage from occupational exposure to car paints

Villalba-Campos, Mónica; Ramírez-Clavijo, Sandra; Sánchez-Corredor, Magda Carolina; Rondón-Lagos, Milena; Ibáñez-Pinilla, Milcíades; Palma, Ruth Marién; Varona-Uribe, Marcela; Chuaire-Noack, Lilian

doi:10.1186/s12995-016-0123-8

Cited by 17 publications

(17 citation statements)

References 26 publications

(33 reference statements)

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“…The MN formation did not correlate with the ccf‐DNA amount in the blood, which might indicate different mechanisms are responsible for both parameters. A similar finding was found in a car‐paint exposed population in which ccf‐DNA and DNA damage were measured; suggesting that lack of correlation between DNA damage and ccf‐DNA can be explained by the adaptive response of ccf‐DNA and the survival mechanisms of the damaged cells [Villalba‐Campos et al, ].…”

Section: Discussionsupporting

confidence: 67%

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Çayır

Çoşkun

Çobanoğlu

2017

Environ and Mol Mutagen

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Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis-block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf-DNA) in the blood of pesticide-exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf-DNA levels using a Qubit fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide-exposed individuals were significantly higher than non-exposed individuals. Apart from MNL, BNMN and CBPI, a season-related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf-DNA level in the pesticide-exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf-DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf-DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf-DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161-169, 2018. © 2017 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 67%

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Çayır

Çoşkun

Çobanoğlu

2017

Environ and Mol Mutagen

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“…Significantly elevated total cfDNA levels have been demonstrated in greenhouse workers exposed to pesticides for 5–15 years vs. controls [ 72 ], while increased cfDNA levels coupled with increased DNA damage were demonstrated in workers exposed to toxic paints in automobile paint shops [ 73 ]. Conversely, another study demonstrated a much lower level of cfDNA in nuclear-site workers exposed either to gamma-neutron or chronic tritium β-radiation [ 74 ].…”

Section: Measurement Of Total Cfdna Levels In Different Contextsmentioning

confidence: 99%

New Perspectives on the Importance of Cell-Free DNA Biology

et al. 2022

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Body fluids are constantly replenished with a population of genetically diverse cell-free DNA (cfDNA) fragments, representing a vast reservoir of information reflecting real-time changes in the host and metagenome. As many body fluids can be collected non-invasively in a one-off and serial fashion, this reservoir can be tapped to develop assays for the diagnosis, prognosis, and monitoring of wide-ranging pathologies, such as solid tumors, fetal genetic abnormalities, rejected organ transplants, infections, and potentially many others. The translation of cfDNA research into useful clinical tests is gaining momentum, with recent progress being driven by rapidly evolving preanalytical and analytical procedures, integrated bioinformatics, and machine learning algorithms. Yet, despite these spectacular advances, cfDNA remains a very challenging analyte due to its immense heterogeneity and fluctuation in vivo. It is increasingly recognized that high-fidelity reconstruction of the information stored in cfDNA, and in turn the development of tests that are fit for clinical roll-out, requires a much deeper understanding of both the physico-chemical features of cfDNA and the biological, physiological, lifestyle, and environmental factors that modulate it. This is a daunting task, but with significant upsides. In this review we showed how expanded knowledge on cfDNA biology and faithful reverse-engineering of cfDNA samples promises to (i) augment the sensitivity and specificity of existing cfDNA assays; (ii) expand the repertoire of disease-specific cfDNA markers, thereby leading to the development of increasingly powerful assays; (iii) reshape personal molecular medicine; and (iv) have an unprecedented impact on genetics research.

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“…Furthermore, their workday may extend beyond the eight hours a day established by Colombian law, conditions that also increase the risks of genotoxic exposure. Although some studies have been conducted worldwide on this topic, including Colombia 20,23 , the relationship between the CYP2E1 polymorphisms and the presence of micronuclei in car paint shop workers have not been examined, which could be used as biomarkers of genotoxic damage and predictors of cancer risk. Consequently, it is necessary to characterize the health and work conditions of individuals occupationally exposed to organic solvents used in car paint workshops in Bogota, Colombia, to alert to the possible onset and extent of early genetic damage, as well as to identify the risk of long-term health problems and to take preventive actions aimed at promoting health.…”

Section: Open Access Introductionmentioning

confidence: 99%

“…Además, su jornada laboral puede extenderse más allá de las ocho horas diarias que establece la ley colombiana, lo que incrementa los riesgos asociados a la exposición genotóxica. A pesar de que se han desarrollado algunos estudios en diversos países del mundo sobre este tópico, incluyendo Colombia 20 , 23 , la relación entre los polimorfismos CYP2E1 y la presencia de micronúcleos en trabajadores de pintura de carros no ha sido examinada, lo que puede ser usado como un biomarcador de daño genotóxico y predictor de riesgo de cáncer. Por consiguiente, es necesario caracterizar la salud y condiciones de trabajo de individuos expuestos ocupacionalmente a solventes orgánicos usados en talleres de pintura automotriz en Bogotá, Colombia, para alertar sobre los posibles inicios de daño genético temprano, así como la identificación de los riesgos de generar problemas a largo plazo en la salud y tomar acciones preventivas que apuntan a la promoción de la salud.…”

Section: Introductionunclassified

Biomarkers of susceptibility and effect in car painters exposed to organic solvents

et al. 2020

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Introduction: Car painters are routinely exposed to organic solvents classified as carcinogenic and mutagenic substances. Objective: To characterize the population susceptibility and evaluate the genotoxic effects of exposure to organic solvents. Methods: A cross-sectional study comparing a group of car painters exposed to organic solvents with a non-exposed group. CYP2E1 polymorphisms and the presence of micronuclei in lymphocytes were determined. Results: One hundred twenty-two workers participated in the study: 62 who worked in car paint shops and were exposed to solvents, and 60 who were not exposed. There were statistically significant differences between the two groups regarding micronucleated cells and nucleoplasmic bridges frequencies (p=0.042 and p=0.046, respectively; exact likelihood ratio). Significant differences were found at the interaction between the CYP2E1 genotype c1c1 and occupational exposure to solvents, with higher frequencies of micronuclei (p= 0.013) and micronucleated cells (p= 0.015). However, when the frequencies of micronuclei, micronucleated cells and nucleoplasmic bridges in the exposure group were compared between the c1c1 and c2c2/c1c2 allele groups of the CYP2E1 polymorphism, statistically significant differences were found. Conclusions: This study confirms that when workers with CYP2E1 polymorphisms, specifically the c1c1 genotype, are exposed to organic solvents, they are more likely to have somatic cell mutations, a condition associated with increased susceptibility to diseases such as cancer

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Quantification of cell-free DNA for evaluating genotoxic damage from occupational exposure to car paints

Cited by 17 publications

References 26 publications

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

New Perspectives on the Importance of Cell-Free DNA Biology

Biomarkers of susceptibility and effect in car painters exposed to organic solvents

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