Quantification of Cell Death Using an Impedance-Based Microfluidic Device

Mansoorifar, Amin; Koklu, Anil; Beşkök, Ali

doi:10.1021/acs.analchem.8b05890

Cited by 31 publications

(22 citation statements)

References 35 publications

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“…Mansoorifar et al. constructed a series of cell capture and characterization devices utilizing a SU‐8 epoxy microwell array (30 μm microwell diameter) with their well‐developed gold electrode impedance platform [80,81]. The group used their dielectric spectroscopy method to optimize frequency ranges to achieve the most effective DEP forces.…”

Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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The 20th century has seen tremendous innovation of dielectrophoresis (DEP) technologies, with applications being developed in areas ranging from industrial processing to micro‐ and nanoscale biotechnology. From 2010 to present day, there have been 981 publications about DEP. Of over 2600 DEP patents held by the United States Patent and Trademark Office, 106 were filed in 2019 alone. This review focuses on DEP‐based technologies and application developments between 2010 and 2020, with an aim to highlight the progress and to identify potential areas for future research. A major trend over the last 10 years has been the use of DEP techniques for biological and clinical applications. It has been used in various forms on a diverse array of biologically derived molecules and particles to manipulate and study them including proteins, exosomes, bacteria, yeast, stem cells, cancer cells, and blood cells. DEP has also been used to manipulate nano‐ and micron‐sized particles in order to fabricate different structures. The next 10 years are likely to see the increase in DEP‐related patent applications begin to result in a greater level of technology commercialization. Also during this time, innovations in DEP technology will likely be leveraged to continue the existing trend to further biological and medical‐focused applications as well as applications in microfabrication. As a tool leveraged by engineering and imaginative scientific design, DEP offers unique capabilities to manipulate small particles in precise ways that can help solve problems and enable scientific inquiry that cannot be addressed using conventional methods.

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Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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“…[17] Within biology, EIS is gaining popularity as a label-free real-time sensing technique, particularly for monitoring of epithelial barrier tissues. [18] Cell growth, [19] cell death, [20] and the changing properties of layers of cells as they differentiate, for instance, toward an osteogenic lineage, [21,22] have also been monitored using EIS.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Biomimetic Deposition of Calcium Phosphate in Real Time Using Complex Capacitance

Guttenplan

Bormans

Birgani

et al. 2021

Physica Status Solidi (a)

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Deposition of minerals, particularly calcium phosphates such as hydroxyapatite, is an important process in the formation of hard tissues such as bone. Herein, a new, affordable, straightforward and nondestructive method based on complex capacitance spectroscopy, an application of electrochemical impedance spectroscopy, is described which allows repeated and real‐time measurements of the same sample throughout the calcium phosphate deposition process. In contrast with end‐point assays which require large numbers of samples to obtain useful time‐course data, this method allows the kinetics of deposition to be measured using a single sample by measuring the impedance of a pair of interdigitated electrodes at a range of frequencies as the layer of mineral is deposited. Changes in the complex capacitance curve over time with deposition can be compared with images of the coating deposited on model substrates, and show different behavior depending on the composition of the coating and the conditions of deposition.

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“…Figure 2 shows an example of a relatively rapid decline in cell health, as inferred from an increase in transmembrane current magnitude during a stimulation pulse sequence. Membrane resistance was calculated from changes in transmembrane current under the assumption of Ohmic resistance, which has previously been shown to be a good approximator of cell injury [3,[25][26][27]. In particular, a decrease in membrane resistance has been correlated with conformational maladies in the membrane (e.g.…”

Section: Stimulation and Recordingmentioning

confidence: 99%

Thermal damage threshold of neurons during infrared stimulation

Brown

Needham

Begeng

et al. 2020

Biomed. Opt. Express

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In infrared neural stimulation (INS), laser-evoked thermal transients are used to generate small depolarising currents in neurons. The laser exposure poses a moderate risk of thermal damage to the target neuron. Indeed, exogenous methods of neural stimulation often place the target neurons under stressful non-physiological conditions, which can hinder ordinary neuronal function and hasten cell death. Therefore, quantifying the exposure-dependent probability of neuronal damage is essential for identifying safe operating limits of INS and other interventions for therapeutic and prosthetic use. Using patch-clamp recordings in isolated spiral ganglion neurons, we describe a method for determining the dose-dependent damage probabilities of individual neurons in response to both acute and cumulative infrared exposure parameters based on changes in injection current. The results identify a local thermal damage threshold at approximately 60°C, which is in keeping with previous literature and supports the claim that damage during INS is a purely thermal phenomenon. In principle this method can be applied to any potentially injurious stimuli, allowing for the calculation of a wide range of dose-dependent neural damage probabilities. Unlike histological analyses, the technique is well-suited to quantifying gradual neuronal damage, and critical threshold behaviour is not required.

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Quantification of Cell Death Using an Impedance-Based Microfluidic Device

Cited by 31 publications

References 35 publications

Dielectrophoresis: Developments and applications from 2010 to 2020

Dielectrophoresis: Developments and applications from 2010 to 2020

Measurement of Biomimetic Deposition of Calcium Phosphate in Real Time Using Complex Capacitance

Thermal damage threshold of neurons during infrared stimulation

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