Quantification of Bleomycin-Induced Murine Lung Damage In Vivo With Micro-Computed Tomography

Cavanaugh, Dawn; Travis, Elizabeth L.; Price, Roger E.; Gladish, Gregory W.; White, R. Allen; Wang, Min; Cody, Dianna D.

doi:10.1016/j.acra.2006.08.011

Cited by 25 publications

(22 citation statements)

References 13 publications

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“…Several studies have reported the use of plethysmography following bleomycin treatment [40,43,44,45,46,47,48,49,50]. Of the reported studies, the majority utilized invasive plethysmography on anaesthetized mice supported by mechanical ventilation at a rate of 150 breaths/min, a tidal volume of 7.5-8 ml/kg and a positive end-expiratory pressure of approximately 3-4 cm H 2 O [40,43,44,50].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

et al. 2011

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Background: Assessment of deterioration of lung function in animal models of respiratory disease traditionally relies upon quantitating biochemical markers. Plethysmography is a technique for measuring lung function that includes invasive and non-invasive methodologies. Objectives: This study used whole-body barometric plethysmography to characterize change(s) in respiratory physiology of C57BL/6 mice following bleomycin administration. Methods: Cohorts of animals were culled at 3, 7, 14 and 28 days to semi-quantitatively score the lung for fibrosis, and quantitate levels of hydroxyproline in the lung. We have described in detail the response of C57BL/6 mice to bleomycin. Results: Bleomycin-treated mice had reduced minute volume (p < 0.05) and an increased total breathing cycle time (p < 0.0001), which consisted of a shortened inspiration time (p < 0.01) and an extended expiration time (p < 0.0001). Conclusions: We have demonstrated that plethysmography can be a primary indicator of the development of respiratory disease in the mouse and would thus be suitable in assessing potential therapies since any truly effective treatment should elicit restoration of respiratory parameters in addition to improving traditional biochemical and histological indices of lung function.

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Section: Discussionmentioning

confidence: 99%

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

et al. 2011

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“…In vivo data obtained from MDCT and magnetic resonance imaging (MRI) have been compared with postoperative or postmortem micro-CT images of excised lung specimens and to morphometry (179,195,196). In one example, excised lungs were air-inflated at a constant transpulmonary pressure while suspended in liquid nitrogen fumes (71).…”

Section: Micro-ctmentioning

confidence: 99%

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Hsia¹,

Hyde²,

Ochs³

et al. 2010

Am J Respir Crit Care Med

779

688

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“…Sectioning, staining with H&E, and video imaging to identify fibrosis in the lung were performed, as previously described (48 -51). The fibrosis score from this technique correlates with the score from noninvasive microcomputed tomography scans (52). Results were obtained from two individuals blinded to the identity of the sections.…”

Section: Fibrosis Scoring In Micementioning

confidence: 99%

Reduction of Bleomycin-Induced Pulmonary Fibrosis by Serum Amyloid P

Pilling

Roife

Wang

et al. 2007

The Journal of Immunology

Self Cite

220

246

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Fibrotic diseases such as scleroderma, severe chronic asthma, pulmonary fibrosis, and cardiac fibrosis kill tens of thousands of people each year in the U.S. alone. Growing evidence suggests that in fibrotic lesions, a subset of blood monocytes enters the tissue and differentiates into fibroblast-like cells called fibrocytes, causing tissue dysfunction. We previously found that a plasma protein called serum amyloid P (SAP) inhibits fibrocyte differentiation in vitro. Bleomycin treatment is a standard model for pulmonary fibrosis, and causes an increase in collagen, fibrocytes, and leukocytes in the lungs, and a decrease in peripheral blood hemoglobin oxygen saturation. We find that injections of rat SAP in rats reduce all of the above bleomycin-induced changes, suggesting that the SAP injections reduced the bleomycin-induced pulmonary fibrosis. We repeated these studies in mice, and find that injections of murine SAP decrease bleomycin-induced pulmonary fibrosis. To confirm the efficacy of SAP treatment, we used a delayed treatment protocol using SAP from day 7 to 13 only, and then measured fibrosis at day 21. Delayed SAP injections also reduce the bleomycin-induced decrease in peripheral blood hemoglobin oxygen saturation, and an increase in lung collagen, leukocyte infiltration, and fibrosis. Our data suggest the possibility that SAP may be useful as a therapy for pulmonary fibrosis in humans.

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Quantification of Bleomycin-Induced Murine Lung Damage In Vivo With Micro-Computed Tomography

Cited by 25 publications

References 13 publications

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

Assessment of Respiratory Physiology of C57BL/6 Mice following Bleomycin Administration Using Barometric Plethysmography

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Reduction of Bleomycin-Induced Pulmonary Fibrosis by Serum Amyloid P

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