An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Hsia, Connie C. W.; Hyde, Dallas M.; Ochs, Matthias; Weibel, Ewald R.

doi:10.1164/rccm.200809-1522st

Cited by 774 publications

(704 citation statements)

References 187 publications

Supporting

Mentioning

688

Contrasting

Unclassified

Order By: Relevance

“…We were able to show that applying the optical disector [35,155] to tomographic data yields results equal to results obtained from classic histological sections. On classic histological sections, the alveolar number has been assessed in relation to the total lung volume using a computer assisted stereological toolbox (newCAST, Visiopharm A/S, Performing this stereological evaluation on tomographic data offers an increased insight into the structure of the lung.…”

Section: Stepanizermentioning

confidence: 63%

“…Traditionally, quantitative information and physical properties of the lung structure are obtained using stereology [34]. Stereology refers to the mathematical methods for analyzing these properties of an irregular three-dimensional structure using two-dimensional planar sections obtained by physical or optical imaging techniques [35].…”

Section: The Need For Tomographymentioning

confidence: 99%

“…The method has been published in the Journal of Applied Physiology and is based on a Finite Element (FE) analysis of the tomographic datasets recorded at TOMCAT. The datasets have been segmented using an iteratively determined threshold. This threshold was chosen to match computed morphological parameters of the resulting three-dimensional reconstruction with the stereologically determined real morphological parameters of samStereology refers to the mathematical methods for defining physical properties of irregular three-dimensional structures using two-dimensional sections obtained by physical or optical imaging techniques [35].…”

Section: Chapter 5 -Multimodal Imagingmentioning

confidence: 99%

“…The STEPanizer is a simple, web-based tool for manual stereological assessment of digital images developed by Dr. Stefan Tschanz at the Institute of Anatomy, University of Bern. The beta-version of the STEPanizer with enabled disector-which according to Hsia et al [35] enables accurate measuring of alveolar number and volume-was used to count the appearance or disappearance of septal bridges in the tomographic datasets.…”

Section: Stepanizermentioning

confidence: 99%

“…Since tomographic images are inherently a stack of slices with a known distance to each other, adjacent slices can be used for disector-type analysis [35].…”

Section: Stepanizermentioning

confidence: 99%

See 4 more Smart Citations

High resolution tomographic imaging of the alveolar region of the mammalian lung

Haberthür¹

2010

SciVee

View full text Add to dashboard Cite

Section: Stepanizermentioning

confidence: 63%

Section: The Need For Tomographymentioning

confidence: 99%

Section: Chapter 5 -Multimodal Imagingmentioning

confidence: 99%

Section: Stepanizermentioning

confidence: 99%

“…Since tomographic images are inherently a stack of slices with a known distance to each other, adjacent slices can be used for disector-type analysis [35].…”

Section: Stepanizermentioning

confidence: 99%

See 3 more Smart Citations

High resolution tomographic imaging of the alveolar region of the mammalian lung

Haberthür¹

2010

SciVee

View full text Add to dashboard Cite

Anatomy, Physiology, and Pathology in Imaging

2019

Hendee's Physics of Medical Imaging, Fifth Edition

View full text Add to dashboard Cite

Is Length an Appropriate Estimator to Characterize Pulmonary Alveolar Capillaries? A Critical Evaluation in the Human Lung

Mühlfeld

Weibel

Hahn

et al. 2010

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

Stereological estimations of total capillary length have been used to characterize changes in the alveolar capillary network (ACN) during developmental processes or pathophysiological conditions. Here, we analyzed whether length estimations are appropriate to describe the 3D nature of the ACN. Semi-thin sections of five human lungs, previously investigated by Gehr et al. (Respir Physiol 1978; 32:121-140), were used to estimate alveolar capillary length using a ''design-based'' or a ''model-based'' stereological approach. The design-based approach involves counting of capillary profiles related to a defined area of the reference space. The model-based approach bases on the assumption that capillaries are round tubes and length was calculated from capillary volume and surface area. The modelbased approach provided a mean of 6,950 km (SD: 3,108 km) for total capillary length, the design-based approach resulted in a mean of 2,746 km (SD: 722 km). Because of the geometry of the ACN both approaches carry an unpredictable bias. The bias incurred by the design-based approach is proportional to the ratio between radius and length of the capillary segments in the ACN, the number of branching points and the winding of the capillaries. The model-based approach is biased because of the real noncylindrical shape of capillaries and the network structure. In conclusion, the estimation of the total length of capillaries in the ACN cannot be recommended as the geometry of the ACN does not fulfill the requirements for stereological length estimation. Until new methods are being developed, the unbiased estimates of capillary volume, and surface area should be preferred. Anat Rec, 293:1270Rec, 293: -1275Rec, 293: , 2010. V V C 2010 Wiley-Liss, Inc.Key words: stereology; capillary length; human lung; angiogenesisStereology is a powerful, efficient, and reliable set of methods for analyzing the morphology of the lung quantitatively (Ochs, 2006;Weibel et al., 2007; Hsia et al., in press). First-order stereology can provide hard biological data about the number, length, surface area, or volume of the object of interest. In this article, we critically evaluate length as a possible parameter to characterize pulmonary capillaries.Grant sponsor: Excellence Cluster Cardiopulmonary System (ECCPS).

show abstract

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Cited by 774 publications

References 187 publications

High resolution tomographic imaging of the alveolar region of the mammalian lung

High resolution tomographic imaging of the alveolar region of the mammalian lung

Anatomy, Physiology, and Pathology in Imaging

Is Length an Appropriate Estimator to Characterize Pulmonary Alveolar Capillaries? A Critical Evaluation in the Human Lung

Contact Info

Product

Resources

About