Quantification of 6-deoxy-6-demethyl-4-dedimethylaminotetracycline (COL-3) in human plasma using liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Chen, Yu-Luan; Hanson, Glenn D.; Weng, Naidong; Powala, Christopher; Zerler, Brad

doi:10.1016/s1570-0232(03)00419-7

Cited by 4 publications

(9 citation statements)

References 6 publications

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“…Huang et al used LC-UV to detect the down-field matrix peaks that caused ion suppression for the analyte of interest and modified the chromatographic condition [113]. Another approach for assessing consistent matrix effect among the individual matrix lots is to measure the consistency for the results obtained from sample from individual matrix lot (typically n > 6) [114][115][116][117][118][119][120][121][122][123]. One could argue that as long as the results (both back-calculated concentration and MS response) are consistent among the tested lots, matrix effects would not likely contribute negatively to the quantitation.…”

Section: Matrix Effects and Recoverymentioning

confidence: 99%

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

Zhou¹,

Song²,

Tang³

et al. 2005

CPA

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Swift growth in the use of LC-MS/MS for the analysis of drugs in biological matrices has been compelled by the need for timely and high-quality data at many stages in drug discovery and development process: from high throughput screening of drug candidates and rapid data generation for pre-clinical studies to almost 'real-time' analysis of clinical samples. Prompt and rational method development, validation, and transfer play a pivotal role in achieving the goals of "faster, better, and cheaper" for pharmacokinetic studies since this could easily account for more than 50% of the time and labor resources for a moderate-sized project. Strategy for rational method development, validation and transfer has been largely kept as institutional knowledge but rarely appeared in literature. In this review article, strategies for developing and validating robust high throughput LC-MS/MS methods will be critically reviewed and discussed. Automated sample preparation, fast chromatography, minimization of matrix effects, and strategy of narrowing the gap between validation and incurred sample analysis are just a few topics covered in this review. Other interesting approaches for improving method efficiency and ruggedness such as direct injection SPE and liquid/liquid extracts as well as multiplexing of LC columns will also be discussed. Potential pitfalls during method development and validation are pointed out. At the end, the question "how fast is fast enough and how fast is too fast?" will be answered after considering all aspects of the method development and validation.

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Section: Matrix Effects and Recoverymentioning

confidence: 99%

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

Zhou¹,

Song²,

Tang³

et al. 2005

CPA

View full text Add to dashboard Cite

show abstract

“…Utilizing the Waters Alliance HPLC, it was necessary to incorporate an extended needle wash and a gradient run (22 min) was necessary to eliminate carryover. The cycle time is 1.25 times shorter than the initial HPLC/MS method but 4 times longer than the LC/MS/MS method [5,6]. Chromatograms representing blank plasma (Fig.…”

Section: Analytical Conditionsmentioning

confidence: 99%

“…The calculated signal to noise ratio of the COL-3 peak is approximately 12:1 at 75 ng/ml, the limit of quantitation. The sensitivity of this method is 2.5 times less than both the HPLC/MS and HPLC/MS/MS methods but utilizes the more readily available HPLC/UV technique [5,6]. The signal to noise ratio for chrysin is approximately 180:1.…”

Section: Analytical Conditionsmentioning

confidence: 99%

“…Two published methods quantitated COL-3 in the concentration range of 30-10,000 ng/ml [5,6]. The first method utilized a HPLC/MS and two standard curves [5], while the second method utilized a HPLC/MS/MS and one standard curve [6].…”

Section: Introductionmentioning

confidence: 99%

“…The first method utilized a HPLC/MS and two standard curves [5], while the second method utilized a HPLC/MS/MS and one standard curve [6]. The concentration range of both assays was sufficient to characterize the pharmacokinetics of COL-3 for up to 1 week and weekly pre-treatment trough levels after administration of doses of 36-98 mg/m 2 in a phase I clinical trial [7,8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A sensitive method for determination of COL-3, a chemically modified tetracycline, in human plasma using high-performance liquid chromatography and ultraviolet detection

Rudek¹,

Hartke²,

Zabelina³

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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Current literature in mass spectrometry

2004

J. Mass Spectrom.

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Quantification of 6-deoxy-6-demethyl-4-dedimethylaminotetracycline (COL-3) in human plasma using liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Cited by 4 publications

References 6 publications

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

A sensitive method for determination of COL-3, a chemically modified tetracycline, in human plasma using high-performance liquid chromatography and ultraviolet detection

Current literature in mass spectrometry

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