Quantification, Morphology and Ultrastructure of Preantral Follicles of Buffalo (<i>Bubalus bubalis</i>) Foetuses

Santos, S. S. D.; Ferreira, María Auxiliadora Pantoja; Lima, Mys; Sampaio, Rafael Vilar; Cordeiro,; Silva, Tvg; Costa, N. N.; Miranda,; Ohashi, O. M.

doi:10.1111/j.1439-0531.2010.01616.x

Cited by 10 publications

(14 citation statements)

References 25 publications

(37 reference statements)

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“…Although we did not directly compare cattle and buffalo ovaries, the number of follicles isolated in this study at 3 to 7 months of fetal age was similar to that previously reported in cattle [19], but higher than in buffalo fetuses of the same age [34]. Conversely, the results obtained in regard to in situ follicular quantification in this study were similar not only to those previously reported for Bos indicus [23], but also to buffalo [22] fetuses. Moreover, in agreement with previous studies, there was large variation in follicle population among animals of the same age and among various fetal ages [3,19,20].…”

Section: Discussionsupporting

confidence: 91%

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Characterization of folliculogenesis and the occurrence of apoptosis in the development of the bovine fetal ovary

Santos¹,

Ferreira²,

Pinto³

et al. 2013

Theriogenology

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The aim of this research was to perform in situ quantification, morphometry evaluation, and apoptosis analysis of ovarian follicular wall cells in mechanically isolated follicles obtained from ovaries of bovine fetuses (Bos taurus indicus) between 3 and 9 months of age. Apoptosis was evaluated using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. The number of isolated follicles increased from 3 months onward (102.5 ± 141.1, mean ± SEM), peaked at 6 months (12855.0 ± 9030.1), and then decreased by 7 months (3208.7 ± 3249.5), consistent with atresia occurring at these stages. Follicular density was greatest at 4 months, consistent with a sudden boost in follicular activity independent of a corresponding increase in ovarian size. Antral follicles were first observed at 5 months. As fetal age increased, there was a tendency for the percentage of primordial and primary follicles to decrease, and the percentage of secondary follicles to increase. However, the high variability (P < 0.05) for all follicle populations up to 5 months of age precluded further interpretation of these results. Oocyte diameter increased from the primordial (23.6 ± 4.4 μm) to the secondary follicular stages (38.0 ± 14.9 μm). Apoptosis was observed in ovaries from all fetal ages analyzed. We concluded that preantral follicles could be isolated from bovine fetuses by 3 months of age, with apoptosis affecting ovarian follicular dynamics throughout fetal life.

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Section: Discussionsupporting

confidence: 91%

“…In the adult bovine ovary, TUNEL and caspase assays have also been used to demonstrate the role of apoptosis in follicles undergoing atresia [18]. Although the ovarian follicular population has been described [19][20][21][22][23], the incidence of apoptosis in the bovine fetal ovary has apparently not been reported.…”

Section: Introductionmentioning

confidence: 99%

Characterization of folliculogenesis and the occurrence of apoptosis in the development of the bovine fetal ovary

Santos¹,

Ferreira²,

Pinto³

et al. 2013

Theriogenology

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“…The existence of cellular junctions between granulosa cells and oocyte enclosed into early PFs promotes the transfer of nutrients and metabolites to the oocyte and introduces low molecular weight signalling molecules that are essential for normal oocyte development (Santos et al. ().…”

Section: Discussionmentioning

confidence: 99%

Morphological Characterization of the Ovarian Preantral Follicle Population of Collared Peccaries (Tayassu tajacuLinnaeus, 1758)

Lima

Santos

Luz

et al. 2012

Anat. Histol. Embryol.

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The aim of this research was to characterize the preantral ovarian follicular population in collared peccaries (Tayassu tajacu) using light and electron microscopy. Ovaries from six mature females were collected and further fixed for histological and ultrastructural analysis. A total of 33273.45 AE 5789.99 preantral follicles (PFs) were estimated for the population in each ovary. Most preantral follicles were primordial (91.56%), followed by primary (6.29%) and secondary (2.15%) ones. Most PFs were morphologically normal (94.4%), and only a few were atretic (5.6%). At histology assessment, amounts of lipid droplets were observed into the oocyte cytoplasm, which was confirmed through ultrastructural analysis. This work characterizes for the first time the ovarian population of preantral follicles, total and per category, in collared peccaries (Tayassu tajacu). The general follicles featured at primordial, primary and secondary categories are very similar to those described for other species.

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“…All reagents for the electron microscopy were purchased from Electron Microscopy Sciences (Hatfield, PA, USA) and samples were processed as described in previous studies [20][21][22][23][24][25][26][27][28][29] with some modifications. Primordial, primary, secondary, antral follicles, and in vitro matured oocytes were evaluated by TEM.…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes

Kere

Liu

Chen

et al. 2020

Animals

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This study aimed to investigate ultrastructural changes of growing porcine oocytes and in vitro maturated oocytes. Light microscopy was used to characterize and localize the primordial, primary, secondary, and tertiary follicles. During oocyte growth and maturation, the morphology of mitochondria was roundish or ovoid in shape depending on the differentiation state, whereas their mean diameters oscillated between 0.5 and 0.7 µm, respectively, from primary and secondary follicles. Hooded mitochondria were found in the growing oocytes of the tertiary follicles. In addition to the pleomorphism of mitochondria, changes in the appearance of lipid droplets were also observed, along with the alignment of a single layer of cortical granules beneath the oolemma. In conclusion, our study is apparently the first report to portray morphological alterations of mitochondria that possess the hooded structure during the growth phase of porcine oocytes. The spatiotemporal and intrinsic changes during oogenesis/folliculogenesis are phenomena at the ultrastructural or subcellular level of porcine oocytes, highlighting an in-depth understanding of oocyte biology and impetus for future studies on practical mitochondrion replacement therapies for oocytes.

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Quantification, Morphology and Ultrastructure of Preantral Follicles of Buffalo (Bubalus bubalis) Foetuses

Cited by 10 publications

References 25 publications

Characterization of folliculogenesis and the occurrence of apoptosis in the development of the bovine fetal ovary

Characterization of folliculogenesis and the occurrence of apoptosis in the development of the bovine fetal ovary

Morphological Characterization of the Ovarian Preantral Follicle Population of Collared Peccaries (Tayassu tajacuLinnaeus, 1758)

Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes

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