Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes

Kere, Michel; Liu, Pan-Chen; Chen, Yuh-Kun; Chao, Pei-Chi; Tsai, Li-Kuang; Yeh, Ting‐Yu; Siriboon, Chawalit; Intawicha, Payungsuk; Lo, Neng-Wen; Chiang, Hung‐Ting; Fan, Yang-Kwang; Ju, Jyh-Cherng

doi:10.3390/ani10040664

Cited by 3 publications

(3 citation statements)

References 46 publications

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Section: Cell Metabolism Correlates With Mitochondrial Morphodynamicsmentioning

confidence: 99%

“…Besides their spatial and morphological heterogeneity in mature tissues, mitochondria are subjected to changes during tissue differentiation and maturation (Porter et al, 2011;Kere et al, 2020), as reviewed in Ding et al (2021), Garbern & Lee (2021) for pluripotent stem cell-derived cardiomyocytes. Although cardiomyocyte mitochondria are fragmented and clustered around the nucleus at early embryonic stage, they then become long and rod-shaped after 13.5 weeks.…”

Section: Cell Metabolism Regulates Mitochondrial Morphodynamicsmentioning

confidence: 99%

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Mitochondria: At the crossroads between mechanobiology and cell metabolism

Villard

Manneville

2023

Biology of the Cell

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Metabolism and mechanics are two key facets of structural and functional processes in cells, such as growth, proliferation, homeostasis and regeneration. Their reciprocal regulation has been increasingly acknowledged in recent years: external physical and mechanical cues entail metabolic changes, which in return regulate cell mechanosensing and mechanotransduction. Since mitochondria are pivotal regulators of metabolism, we review here the reciprocal links between mitochondrial morphodynamics, mechanics and metabolism. Mitochondria are highly dynamic organelles which sense and integrate mechanical, physical and metabolic cues to adapt their morphology, the organization of their network and their metabolic functions. While some of the links between mitochondrial morphodynamics, mechanics and metabolism are already well established, others are still poorly documented and open new fields of research. First, cell metabolism is known to correlate with mitochondrial morphodynamics. For instance, mitochondrial fission, fusion and cristae remodeling allow the cell to fine‐tune its energy production through the contribution of mitochondrial oxidative phosphorylation and cytosolic glycolysis. Second, mechanical cues and alterations in mitochondrial mechanical properties reshape and reorganize the mitochondrial network. Mitochondrial membrane tension emerges as a decisive physical property which regulates mitochondrial morphodynamics. However, the converse link hypothesizing a contribution of morphodynamics to mitochondria mechanics and/or mechanosensitivity has not yet been demonstrated. Third, we highlight that mitochondrial mechanics and metabolism are reciprocally regulated, although little is known about the mechanical adaptation of mitochondria in response to metabolic cues. Deciphering the links between mitochondrial morphodynamics, mechanics and metabolism still presents significant technical and conceptual challenges but is crucial both for a better understanding of mechanobiology and for potential novel therapeutic approaches in diseases such as cancer.

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Section: Cell Metabolism Correlates With Mitochondrial Morphodynamicsmentioning

confidence: 99%

Section: Cell Metabolism Regulates Mitochondrial Morphodynamicsmentioning

confidence: 99%

Mitochondria: At the crossroads between mechanobiology and cell metabolism

Villard

Manneville

2023

Biology of the Cell

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“…In pig oocytes, spherical mitochondria with few cristae are the main pattern of mitochondria observed before maturation, which presumably has only a low metabolic activity of energy [ 39 ]. Hooded mitochondria were rarely seen, in contrast to the more frequently existing shell-like and compartmentalized mitochondria in post-pubertal porcine oocytes [ 40 , 41 ]. A previous study reported that EMF might induce morphological changes in mitochondrial cristae in human pancreatic cancer cells [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of electromagnetic waves on oocyte maturation and embryonic development in pigs

Chen

Tsai

Yeh

et al. 2021

Journal of Reproduction and Development

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Our living environment has been full of electromagnetic radiation (EMR) due to the prevailing electronic devices and equipment. Intermediate frequency electromagnetic field (IF-EMF) or waves constitute a significant part of EMR; therefore, an increasing number of household electrical appliances have become a source of IF-EMF, and concerns about IF-EMF on health are gaining more attention. However, little information is available about its impact on female reproductive traits, such as germ cell viability and early embryonic development, particularly at the cellular and molecular levels. In this study, we used porcine oocytes as a model system to explore the effect of IF-EMF at various intensities on the in vitro maturation (IVM) of oocytes and their subsequent embryonic development. Our results showed that no difference in oocyte maturation rates was detected among groups, but the cleavage and blastocyst rates of parthenotes derived from EMF-treated oocytes decreased with the weaker IF-EMF intensity (25 and 50 Gauss) groups compared to the control group (P < 0.05). For cytoplasmic maturation, the weaker IF-EMF intensity groups also showed a peripheral pattern of mitochondrial distribution resembling that of immature oocytes and increased autophagy activity. No obvious differences in cytoskeletal distribution and total cell numbers of blastocysts were investigated in the four IF-EMF treatments compared to those in the control group. Although the underlying mechanism associated with EMF effects on oocytes and embryos is still elusive, we have demonstrated that low intensity IF-EMF exerts harmful effects on porcine oocytes during the maturation stage, carrying over such effects to their subsequent embryonic development.

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The mechanisms that control the preantral to early antral follicle transition and the strategies to have efficient culture systems to promote their growthin vitro

Nascimento

Barbalho

Barrozo

et al. 2023

Zygote

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Summary Preantral to early antral follicles transition is a complex process regulated by endocrine and paracrine factors, as well as by a precise interaction among oocyte, granulosa cells and theca cells. Understanding the mechanisms that regulate this step of folliculogenesis is important to improve in vitro culture systems, and opens new perspectives to use oocytes from preantral follicles for assisted reproductive technologies. Therefore, this review aims to discuss the endocrine and paracrine mechanisms that control granulosa cell proliferation and differentiation, formation of the antral cavity, estradiol production, atresia, and follicular fluid production during the transition from preantral to early antral follicles. The strategies that promote in vitro growth of preantral follicles are also discussed.

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Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes

Cited by 3 publications

References 46 publications

Mitochondria: At the crossroads between mechanobiology and cell metabolism

Mitochondria: At the crossroads between mechanobiology and cell metabolism

Effects of electromagnetic waves on oocyte maturation and embryonic development in pigs

The mechanisms that control the preantral to early antral follicle transition and the strategies to have efficient culture systems to promote their growthin vitro

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