Quantification and Optimization of Ethanolic Extract Containing the Bioactive Flavonoids from Millettia pulchra Radix

Abstract

Millettia pulchra is traditionally used for treating diseases, including joint pain, fever, anemia, and allergies. It is also a potential resource of natural flavonoid derivatives, which represents major constituents of this plant. This study aimed to isolate the major compounds from M. pulchra radix, develop and validate the HPLC-PDA method to determine their contents, and optimize its extraction. Four major flavonoid derivatives (karanjin, lanceolatin B, 2”,2”-dimethylpyrano-[5″,6″:7,8]-flavone, and pongamol) were isolated using silica gel column chromatography, crystallization techniques in large amounts with high purities (>95%). A simple, accurate high-performance liquid chromatography–photodiode array (HPLC–PDA) detection method has been developed and validated with significantly statistical impacts according to International Conference on Harmonization (ICH) guidelines. The Response Surface Methodology (RSM), Artificial Neural Network (ANN) models were employed to predictive performance and optimization of the extraction process. The optimized conditions for the extraction of major flavonoids were: extraction time (twice), solvent/material ratio (9.5), and ethanol concentration (72.5%). Our research suggests an effective method, which will be helpful for quality control in the pharmaceutical development of this species.

Pongamol Alleviates Neuroinflammation and Promotes Autophagy in Alzheimer’s Disease by Regulating the Akt/mTOR Signaling Pathway

“…10−13 Pongamol, a typical natural flavonoid, is isolated from leguminous plants, such as Pongamia pinnata, licorice, and other herbs. 14 Previous studies have shown that it has a wide range of biological activities, including antioxidant, antidiabetic, antiulcer, and anticancer effects. 15 in a rat model.…”

“…The therapeutic benefits of natural products are gaining increasing recognition due to their therapeutic advantages of multiple targets, multiple links, minimal toxicity, and few side effects. − Pongamol, a typical natural flavonoid, is isolated from leguminous plants, such as Pongamia pinnata, licorice, and other herbs . Previous studies have shown that it has a wide range of biological activities, including antioxidant, antidiabetic, antiulcer, and anticancer effects .…”