Pongamol Prevents Neurotoxicity via the Activation of MAPKs/Nrf2 Signaling Pathway in H2O2-Induced Neuronal PC12 Cells and Prolongs the Lifespan of Caenorhabditis elegans

Wu, Shaojun; Miao, Jie; Zhu, Susu; Wu, Xinyuan; Shi, Jindan; Zhou, Jichao; Xing, Yi; Hu, Kun; Ren, Jie; Yang, Hao

doi:10.1007/s12035-024-04110-x

Cited by 1 publication

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“…Subsequently, the total hippocampus protein (20 mg/sample) was extracted from the tissues with 202 μL of RIPA lysis buffer/protease inhibitor/phosphatase inhibitor = 200:1:1, and ultrasound was performed on the ice for 6 times for 5 s each time and then centrifuged. The steps of protein quantification and WB were carried out according to the previous protocol in our laboratory …”

Section: Methodsmentioning

confidence: 99%

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Pongamol Alleviates Neuroinflammation and Promotes Autophagy in Alzheimer’s Disease by Regulating the Akt/mTOR Signaling Pathway

Hu,

Wu,

et al. 2024

J. Agric. Food Chem.

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Alzheimer's disease (AD), one of the neurodegenerative disorders, is highly correlated with the abnormal hyperphosphorylation of Tau and aggregation of β-amyloid (Aβ). Oxidative stress, neuroinflammation, and abnormal autophagy are key drivers of AD and how they contribute to neuropathology remains largely unknown. The flavonoid compound pongamol is reported to possess a variety of pharmacological activities, such as antioxidant, antibacterial, and anti-inflammatory. This study investigated the neuroprotective effect and its mechanisms of pongamol in lipopolysaccharide (LPS)-induced BV2 cells, d-galactose/ sodium nitrite/aluminum chloride (D-gal/NaNO 2 /AlCl 3 )-induced AD mice, and Caenorhabditis elegans models. Our research revealed that pongamol reduced the release of inflammatory factors IL-1β, TNF-α, COX-2, and iNOS in LPS-induced BV2 cells. Pongamol also protected neurons and significantly restored memory function, inhibited Tau phosphorylation, downregulated Aβ aggregation, and increased oxidoreductase activity in the hippocampus of AD mice. In addition, pongamol reversed the nuclear transfer of NF-κB and increased the levels of Beclin 1 and LC3 II/LC3 I. Most importantly, the anti-inflammatory and promoter autophagy effects of pongamol may be related to the regulation of the Akt/mTOR signaling pathway. In summary, these results showed that pongamol has a potential neuroprotective effect, which greatly enriched the research on the pharmacological activity of pongamol for improving AD.

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Section: Methodsmentioning

confidence: 99%

“…The treatment of UM0001 C. elegans and the steps of mRNA extraction and reverse transcription were carried out according to the previous protocol in our laboratory . The primer sequences for each gene are listed in Tables and .…”

Section: Methodsmentioning

confidence: 99%